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Effects of ginsenoside Rb₁ on skin changes.

Kimura Y, Sumiyoshi M, Sakanaka M - J. Biomed. Biotechnol. (2012)

Bottom Line: Ginseng roots (Panax ginseng CA Meyer) have been used traditionally for the treatment, especially prevention, of various diseases in China, Korea, and Japan.The topical use of ginseng roots to treat skin complaints including atopic suppurative dermatitis, wounds, and inflammation is also described in ancient Chinese texts; however, there have been relatively few studies in this area.Furthermore, to clarify the mechanisms behind these pharmacological actions, human primary keratinocytes and the human keratinocyte cell line HaCaT were used in experiments in vitro.

View Article: PubMed Central - PubMed

Affiliation: Division of Biochemical Pharmacology, Department of Basic Medical Research, Ehime University Graduate School of Medicine, Shitsukawa, Toon City, Ehime 791-0295, Japan. yokim@m.ehime-u.ac.jp

ABSTRACT
Ginseng roots (Panax ginseng CA Meyer) have been used traditionally for the treatment, especially prevention, of various diseases in China, Korea, and Japan. Both experimental and clinical studies suggest ginseng roots to have pharmacological effects in patients with life-style-related diseases such as non-insulin-dependent diabetic mellitus, atherosclerosis, hyperlipidemia, and hypertension. The topical use of ginseng roots to treat skin complaints including atopic suppurative dermatitis, wounds, and inflammation is also described in ancient Chinese texts; however, there have been relatively few studies in this area. In the present paper, we describe introduce the biological and pharmacological effects of ginsenoside Rb₁ isolated from Red ginseng roots on skin damage caused by burn-wounds using male Balb/c mice (in vivo) and by ultraviolet B irradiation using male C57BL/6J and albino hairless (HR-1) mice (in vivo). Furthermore, to clarify the mechanisms behind these pharmacological actions, human primary keratinocytes and the human keratinocyte cell line HaCaT were used in experiments in vitro.

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Effects of ginsenoside Rb1 on skin thickness in chronic UVB-irradiated male hairless (HRM-1) mice [31]. The initial dose of UVB was set at 36 mJ/cm2, which was subsequently increased to 54 mJ/cm2 at weeks 1–4, 72 mJ/cm2 at weeks 4–7, 108 mJ/cm2 at weeks 7–10, and finally 122 mJ/cm2 at weeks 10–12 in male albino hairless HOS: HR-1 mice. The frequency of UVB irradiation was set at three times per week. Ginsenoside Rb1 (100 fg, 10 pg, and 1 ng/mouse) was applied topically to the dorsal region of each mouse every day for 12 weeks. The dorsal skin of the hairless mice was lifted up by pinching gently under anesthetization with pentobarbital, and skin-fold thickness was measured using a Quick Mini caliper. Skin thickness after UVB irradiation was measured every week. Values are the mean ± SE for 6 mice. *Significantly different from vehicle-treated mice, P < 0.05.
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fig9: Effects of ginsenoside Rb1 on skin thickness in chronic UVB-irradiated male hairless (HRM-1) mice [31]. The initial dose of UVB was set at 36 mJ/cm2, which was subsequently increased to 54 mJ/cm2 at weeks 1–4, 72 mJ/cm2 at weeks 4–7, 108 mJ/cm2 at weeks 7–10, and finally 122 mJ/cm2 at weeks 10–12 in male albino hairless HOS: HR-1 mice. The frequency of UVB irradiation was set at three times per week. Ginsenoside Rb1 (100 fg, 10 pg, and 1 ng/mouse) was applied topically to the dorsal region of each mouse every day for 12 weeks. The dorsal skin of the hairless mice was lifted up by pinching gently under anesthetization with pentobarbital, and skin-fold thickness was measured using a Quick Mini caliper. Skin thickness after UVB irradiation was measured every week. Values are the mean ± SE for 6 mice. *Significantly different from vehicle-treated mice, P < 0.05.

Mentions: The topical application of ginsenoside Rb1 at lower doses, 100 fg, 10 pg, and 1 ng/mouse, significantly inhibited the increase in skin thickness induced by UVB irradiation during weeks 2 to 12 compared to the skin thickness of vehicle-treated UVB-irradiated mice (control) (Figure 9).


Effects of ginsenoside Rb₁ on skin changes.

Kimura Y, Sumiyoshi M, Sakanaka M - J. Biomed. Biotechnol. (2012)

Effects of ginsenoside Rb1 on skin thickness in chronic UVB-irradiated male hairless (HRM-1) mice [31]. The initial dose of UVB was set at 36 mJ/cm2, which was subsequently increased to 54 mJ/cm2 at weeks 1–4, 72 mJ/cm2 at weeks 4–7, 108 mJ/cm2 at weeks 7–10, and finally 122 mJ/cm2 at weeks 10–12 in male albino hairless HOS: HR-1 mice. The frequency of UVB irradiation was set at three times per week. Ginsenoside Rb1 (100 fg, 10 pg, and 1 ng/mouse) was applied topically to the dorsal region of each mouse every day for 12 weeks. The dorsal skin of the hairless mice was lifted up by pinching gently under anesthetization with pentobarbital, and skin-fold thickness was measured using a Quick Mini caliper. Skin thickness after UVB irradiation was measured every week. Values are the mean ± SE for 6 mice. *Significantly different from vehicle-treated mice, P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303758&req=5

fig9: Effects of ginsenoside Rb1 on skin thickness in chronic UVB-irradiated male hairless (HRM-1) mice [31]. The initial dose of UVB was set at 36 mJ/cm2, which was subsequently increased to 54 mJ/cm2 at weeks 1–4, 72 mJ/cm2 at weeks 4–7, 108 mJ/cm2 at weeks 7–10, and finally 122 mJ/cm2 at weeks 10–12 in male albino hairless HOS: HR-1 mice. The frequency of UVB irradiation was set at three times per week. Ginsenoside Rb1 (100 fg, 10 pg, and 1 ng/mouse) was applied topically to the dorsal region of each mouse every day for 12 weeks. The dorsal skin of the hairless mice was lifted up by pinching gently under anesthetization with pentobarbital, and skin-fold thickness was measured using a Quick Mini caliper. Skin thickness after UVB irradiation was measured every week. Values are the mean ± SE for 6 mice. *Significantly different from vehicle-treated mice, P < 0.05.
Mentions: The topical application of ginsenoside Rb1 at lower doses, 100 fg, 10 pg, and 1 ng/mouse, significantly inhibited the increase in skin thickness induced by UVB irradiation during weeks 2 to 12 compared to the skin thickness of vehicle-treated UVB-irradiated mice (control) (Figure 9).

Bottom Line: Ginseng roots (Panax ginseng CA Meyer) have been used traditionally for the treatment, especially prevention, of various diseases in China, Korea, and Japan.The topical use of ginseng roots to treat skin complaints including atopic suppurative dermatitis, wounds, and inflammation is also described in ancient Chinese texts; however, there have been relatively few studies in this area.Furthermore, to clarify the mechanisms behind these pharmacological actions, human primary keratinocytes and the human keratinocyte cell line HaCaT were used in experiments in vitro.

View Article: PubMed Central - PubMed

Affiliation: Division of Biochemical Pharmacology, Department of Basic Medical Research, Ehime University Graduate School of Medicine, Shitsukawa, Toon City, Ehime 791-0295, Japan. yokim@m.ehime-u.ac.jp

ABSTRACT
Ginseng roots (Panax ginseng CA Meyer) have been used traditionally for the treatment, especially prevention, of various diseases in China, Korea, and Japan. Both experimental and clinical studies suggest ginseng roots to have pharmacological effects in patients with life-style-related diseases such as non-insulin-dependent diabetic mellitus, atherosclerosis, hyperlipidemia, and hypertension. The topical use of ginseng roots to treat skin complaints including atopic suppurative dermatitis, wounds, and inflammation is also described in ancient Chinese texts; however, there have been relatively few studies in this area. In the present paper, we describe introduce the biological and pharmacological effects of ginsenoside Rb₁ isolated from Red ginseng roots on skin damage caused by burn-wounds using male Balb/c mice (in vivo) and by ultraviolet B irradiation using male C57BL/6J and albino hairless (HR-1) mice (in vivo). Furthermore, to clarify the mechanisms behind these pharmacological actions, human primary keratinocytes and the human keratinocyte cell line HaCaT were used in experiments in vitro.

Show MeSH
Related in: MedlinePlus