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Effects of ginsenoside Rb₁ on skin changes.

Kimura Y, Sumiyoshi M, Sakanaka M - J. Biomed. Biotechnol. (2012)

Bottom Line: Ginseng roots (Panax ginseng CA Meyer) have been used traditionally for the treatment, especially prevention, of various diseases in China, Korea, and Japan.The topical use of ginseng roots to treat skin complaints including atopic suppurative dermatitis, wounds, and inflammation is also described in ancient Chinese texts; however, there have been relatively few studies in this area.Furthermore, to clarify the mechanisms behind these pharmacological actions, human primary keratinocytes and the human keratinocyte cell line HaCaT were used in experiments in vitro.

View Article: PubMed Central - PubMed

Affiliation: Division of Biochemical Pharmacology, Department of Basic Medical Research, Ehime University Graduate School of Medicine, Shitsukawa, Toon City, Ehime 791-0295, Japan. yokim@m.ehime-u.ac.jp

ABSTRACT
Ginseng roots (Panax ginseng CA Meyer) have been used traditionally for the treatment, especially prevention, of various diseases in China, Korea, and Japan. Both experimental and clinical studies suggest ginseng roots to have pharmacological effects in patients with life-style-related diseases such as non-insulin-dependent diabetic mellitus, atherosclerosis, hyperlipidemia, and hypertension. The topical use of ginseng roots to treat skin complaints including atopic suppurative dermatitis, wounds, and inflammation is also described in ancient Chinese texts; however, there have been relatively few studies in this area. In the present paper, we describe introduce the biological and pharmacological effects of ginsenoside Rb₁ isolated from Red ginseng roots on skin damage caused by burn-wounds using male Balb/c mice (in vivo) and by ultraviolet B irradiation using male C57BL/6J and albino hairless (HR-1) mice (in vivo). Furthermore, to clarify the mechanisms behind these pharmacological actions, human primary keratinocytes and the human keratinocyte cell line HaCaT were used in experiments in vitro.

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Related in: MedlinePlus

Effects of ginsenoside Rb1 and bFGF on MCP-1 (a) and histamine (b) production in the exudates of burns in male Balb/c mice [27]. The experiments were performed as described in Figure 3, and then MCP-1 and histamine levels in the filter pellets were measured using mouse MCP-1 and histamine ELISA kits, respectively. Values are the mean ± SE for 6 mice. *Significantly different from control, P < 0.05.
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fig7: Effects of ginsenoside Rb1 and bFGF on MCP-1 (a) and histamine (b) production in the exudates of burns in male Balb/c mice [27]. The experiments were performed as described in Figure 3, and then MCP-1 and histamine levels in the filter pellets were measured using mouse MCP-1 and histamine ELISA kits, respectively. Values are the mean ± SE for 6 mice. *Significantly different from control, P < 0.05.

Mentions: These findings suggest the enhancement of wound healing by ginsenoside Rb1 to be due to the promotion of angiogenesis during the repair process as a result of the stimulation of VEGF production caused by the increase in HIF-1α expression in keratinocytes. Furthermore, the MCP-1 level in the exudates of vehicle-treated (control) mice reached a maximum 1 day after the burn treatment and declined rapidly from day 3. Ginsenoside Rb1 (1 ng per wound) and bFGF (2.5 μg per wound) significantly increased the level of MCP-1 on day 1 compared to that in control mice (Figure 7). Histamine levels in the exudates of the burn wound area increased until day 7. Ginsenoside Rb1 (1 ng per wound) significantly increased the histamine level on day 5 compared to that in control mice (Figure 7). Furthermore, ginsenoside Rb1 (100 fg, 10 pg, and 1 ng per wound) and bFGF (2.5 μg per wound) significantly increased histamine production on day 7 (Figure 7). The facilitating effects of ginsenoside Rb1 may be due to the promotion of angiogenesis via the activation of bFGF through the increase in histamine released from mast cells recruited by the stimulation of MCP-1 production.


Effects of ginsenoside Rb₁ on skin changes.

Kimura Y, Sumiyoshi M, Sakanaka M - J. Biomed. Biotechnol. (2012)

Effects of ginsenoside Rb1 and bFGF on MCP-1 (a) and histamine (b) production in the exudates of burns in male Balb/c mice [27]. The experiments were performed as described in Figure 3, and then MCP-1 and histamine levels in the filter pellets were measured using mouse MCP-1 and histamine ELISA kits, respectively. Values are the mean ± SE for 6 mice. *Significantly different from control, P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303758&req=5

fig7: Effects of ginsenoside Rb1 and bFGF on MCP-1 (a) and histamine (b) production in the exudates of burns in male Balb/c mice [27]. The experiments were performed as described in Figure 3, and then MCP-1 and histamine levels in the filter pellets were measured using mouse MCP-1 and histamine ELISA kits, respectively. Values are the mean ± SE for 6 mice. *Significantly different from control, P < 0.05.
Mentions: These findings suggest the enhancement of wound healing by ginsenoside Rb1 to be due to the promotion of angiogenesis during the repair process as a result of the stimulation of VEGF production caused by the increase in HIF-1α expression in keratinocytes. Furthermore, the MCP-1 level in the exudates of vehicle-treated (control) mice reached a maximum 1 day after the burn treatment and declined rapidly from day 3. Ginsenoside Rb1 (1 ng per wound) and bFGF (2.5 μg per wound) significantly increased the level of MCP-1 on day 1 compared to that in control mice (Figure 7). Histamine levels in the exudates of the burn wound area increased until day 7. Ginsenoside Rb1 (1 ng per wound) significantly increased the histamine level on day 5 compared to that in control mice (Figure 7). Furthermore, ginsenoside Rb1 (100 fg, 10 pg, and 1 ng per wound) and bFGF (2.5 μg per wound) significantly increased histamine production on day 7 (Figure 7). The facilitating effects of ginsenoside Rb1 may be due to the promotion of angiogenesis via the activation of bFGF through the increase in histamine released from mast cells recruited by the stimulation of MCP-1 production.

Bottom Line: Ginseng roots (Panax ginseng CA Meyer) have been used traditionally for the treatment, especially prevention, of various diseases in China, Korea, and Japan.The topical use of ginseng roots to treat skin complaints including atopic suppurative dermatitis, wounds, and inflammation is also described in ancient Chinese texts; however, there have been relatively few studies in this area.Furthermore, to clarify the mechanisms behind these pharmacological actions, human primary keratinocytes and the human keratinocyte cell line HaCaT were used in experiments in vitro.

View Article: PubMed Central - PubMed

Affiliation: Division of Biochemical Pharmacology, Department of Basic Medical Research, Ehime University Graduate School of Medicine, Shitsukawa, Toon City, Ehime 791-0295, Japan. yokim@m.ehime-u.ac.jp

ABSTRACT
Ginseng roots (Panax ginseng CA Meyer) have been used traditionally for the treatment, especially prevention, of various diseases in China, Korea, and Japan. Both experimental and clinical studies suggest ginseng roots to have pharmacological effects in patients with life-style-related diseases such as non-insulin-dependent diabetic mellitus, atherosclerosis, hyperlipidemia, and hypertension. The topical use of ginseng roots to treat skin complaints including atopic suppurative dermatitis, wounds, and inflammation is also described in ancient Chinese texts; however, there have been relatively few studies in this area. In the present paper, we describe introduce the biological and pharmacological effects of ginsenoside Rb₁ isolated from Red ginseng roots on skin damage caused by burn-wounds using male Balb/c mice (in vivo) and by ultraviolet B irradiation using male C57BL/6J and albino hairless (HR-1) mice (in vivo). Furthermore, to clarify the mechanisms behind these pharmacological actions, human primary keratinocytes and the human keratinocyte cell line HaCaT were used in experiments in vitro.

Show MeSH
Related in: MedlinePlus