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Differential expression of matrix metalloproteases in human fibroblasts with different origins.

Lindner D, Zietsch C, Becher PM, Schulze K, Schultheiss HP, Tschöpe C, Westermann D - Biochem Res Int (2012)

Bottom Line: Furthermore, we examined basal expression levels of collagen and different MMPs in these three types of fibroblasts and compared these concerning their site of origin.Interestingly, we found major differences in basal mRNA expression especially for MMP1 and MMP3.In conclusion, fibroblasts show different properties in proliferation and MMP expression regarding their originated tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology and Pneumology, Charité Universitätsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany.

ABSTRACT
Fibroblasts are widely distributed cells and are responsible for the deposition of extracellular matrix (ECM) components but also secrete ECM-degrading matrix metalloproteases. A finely balanced equilibrium between deposition and degradation of ECM is essential for structural integrity of tissues. In the past, fibroblasts have typically been understood as a uniform cell population with comparable functions regardless of their origin. Here, we determined growth curves of fibroblasts derived from heart, skin, and lung and clearly show the lowest proliferation rate for cardiac fibroblasts. Furthermore, we examined basal expression levels of collagen and different MMPs in these three types of fibroblasts and compared these concerning their site of origin. Interestingly, we found major differences in basal mRNA expression especially for MMP1 and MMP3. Moreover, we treated fibroblasts with TNF-α and observed different alterations under these proinflammatory conditions. In conclusion, fibroblasts show different properties in proliferation and MMP expression regarding their originated tissue.

No MeSH data available.


Related in: MedlinePlus

Alteration of MMP expression levels in cardiac, dermal, and pulmonary fibroblasts after treatment with 10 ng/mL TNF-α for 24 hours. Different alteration of collagenases (a), stromelysins (b), and MMP9 (c) expression levels after TNF-α treatment. MMP9 activity was further determined in the cell culture supernatant of cardiac, dermal, and pulmonary fibroblasts. Representative bands of MMP9 activity in dermal and pulmonary cell culture supernatants with or without TNF-α for 72 hours are shown. No MMP9 activity could be detected in cell culture supernatant of cardiac fibroblasts. All mRNA expression levels are shown as absolute expression using the formula 2−ΔCt.
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fig3: Alteration of MMP expression levels in cardiac, dermal, and pulmonary fibroblasts after treatment with 10 ng/mL TNF-α for 24 hours. Different alteration of collagenases (a), stromelysins (b), and MMP9 (c) expression levels after TNF-α treatment. MMP9 activity was further determined in the cell culture supernatant of cardiac, dermal, and pulmonary fibroblasts. Representative bands of MMP9 activity in dermal and pulmonary cell culture supernatants with or without TNF-α for 72 hours are shown. No MMP9 activity could be detected in cell culture supernatant of cardiac fibroblasts. All mRNA expression levels are shown as absolute expression using the formula 2−ΔCt.

Mentions: In more detail, MMP1 expression was increased 2.0-fold, 100-fold, and 6.0-fold in cardiac, dermal, and pulmonary fibroblasts, respectively (Table 2). In Figure 3(a) it is clearly shown that the TNF-α treatment finally leads to a similar mRNA expression of MMP1 in cardiac and dermal fibroblasts, whereas MMP1 is still significantly lower expressed after TNF-α treatment in pulmonary fibroblasts compared to the cardiac expression levels. Concerning the TNF-α-induced change in expression levels of the collagenase MMP8 similar results could be observed. TNF-α leads to an increase of 5.7-fold and 130-fold in cardiac and dermal fibroblasts, respectively, resulting finally in comparable expression levels of MMP8 in cardiac and dermal fibroblasts which demonstrate that no significant difference could be observed after TNF-α treatment in contrast to the properties prior to stimulation. In pulmonary fibroblasts the expression of MMP8 was increased by a factor of 10, but still significantly lower than in cardiac fibroblasts.


Differential expression of matrix metalloproteases in human fibroblasts with different origins.

Lindner D, Zietsch C, Becher PM, Schulze K, Schultheiss HP, Tschöpe C, Westermann D - Biochem Res Int (2012)

Alteration of MMP expression levels in cardiac, dermal, and pulmonary fibroblasts after treatment with 10 ng/mL TNF-α for 24 hours. Different alteration of collagenases (a), stromelysins (b), and MMP9 (c) expression levels after TNF-α treatment. MMP9 activity was further determined in the cell culture supernatant of cardiac, dermal, and pulmonary fibroblasts. Representative bands of MMP9 activity in dermal and pulmonary cell culture supernatants with or without TNF-α for 72 hours are shown. No MMP9 activity could be detected in cell culture supernatant of cardiac fibroblasts. All mRNA expression levels are shown as absolute expression using the formula 2−ΔCt.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303709&req=5

fig3: Alteration of MMP expression levels in cardiac, dermal, and pulmonary fibroblasts after treatment with 10 ng/mL TNF-α for 24 hours. Different alteration of collagenases (a), stromelysins (b), and MMP9 (c) expression levels after TNF-α treatment. MMP9 activity was further determined in the cell culture supernatant of cardiac, dermal, and pulmonary fibroblasts. Representative bands of MMP9 activity in dermal and pulmonary cell culture supernatants with or without TNF-α for 72 hours are shown. No MMP9 activity could be detected in cell culture supernatant of cardiac fibroblasts. All mRNA expression levels are shown as absolute expression using the formula 2−ΔCt.
Mentions: In more detail, MMP1 expression was increased 2.0-fold, 100-fold, and 6.0-fold in cardiac, dermal, and pulmonary fibroblasts, respectively (Table 2). In Figure 3(a) it is clearly shown that the TNF-α treatment finally leads to a similar mRNA expression of MMP1 in cardiac and dermal fibroblasts, whereas MMP1 is still significantly lower expressed after TNF-α treatment in pulmonary fibroblasts compared to the cardiac expression levels. Concerning the TNF-α-induced change in expression levels of the collagenase MMP8 similar results could be observed. TNF-α leads to an increase of 5.7-fold and 130-fold in cardiac and dermal fibroblasts, respectively, resulting finally in comparable expression levels of MMP8 in cardiac and dermal fibroblasts which demonstrate that no significant difference could be observed after TNF-α treatment in contrast to the properties prior to stimulation. In pulmonary fibroblasts the expression of MMP8 was increased by a factor of 10, but still significantly lower than in cardiac fibroblasts.

Bottom Line: Furthermore, we examined basal expression levels of collagen and different MMPs in these three types of fibroblasts and compared these concerning their site of origin.Interestingly, we found major differences in basal mRNA expression especially for MMP1 and MMP3.In conclusion, fibroblasts show different properties in proliferation and MMP expression regarding their originated tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology and Pneumology, Charité Universitätsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany.

ABSTRACT
Fibroblasts are widely distributed cells and are responsible for the deposition of extracellular matrix (ECM) components but also secrete ECM-degrading matrix metalloproteases. A finely balanced equilibrium between deposition and degradation of ECM is essential for structural integrity of tissues. In the past, fibroblasts have typically been understood as a uniform cell population with comparable functions regardless of their origin. Here, we determined growth curves of fibroblasts derived from heart, skin, and lung and clearly show the lowest proliferation rate for cardiac fibroblasts. Furthermore, we examined basal expression levels of collagen and different MMPs in these three types of fibroblasts and compared these concerning their site of origin. Interestingly, we found major differences in basal mRNA expression especially for MMP1 and MMP3. Moreover, we treated fibroblasts with TNF-α and observed different alterations under these proinflammatory conditions. In conclusion, fibroblasts show different properties in proliferation and MMP expression regarding their originated tissue.

No MeSH data available.


Related in: MedlinePlus