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Evaluation of a Novel Rapid Test System for the Detection of Specific IgE to Hymenoptera Venoms.

Pfender N, Lucassen R, Offermann N, Schulte-Pelkum J, Fooke M, Jakob T - J Allergy (Cairo) (2012)

Bottom Line: Sensitivity and specificity of ALFA and correlation of ALFA and ImmunoCAP results were calculated.Results.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Allergy Research Group, Department of Dermatology, University Medical Center Freiburg, Hauptstraße 7, 79104 Freiburg, Germany.

ABSTRACT
Background. The Allergy Lateral Flow Assay (ALFA) is a novel rapid assay for the detection of sIgE to allergens. The objective of this study is the evaluation of ALFA for the detection of sIgE to bee venom (BV) and wasp venom (WV) in insect venom allergic patients. Methods. Specific IgE to BV and WV was analyzed by ALFA, ALLERG-O-LIQ, and ImmunoCAP in 80 insect venom allergic patients and 60 control sera. Sensitivity and specificity of ALFA and correlation of ALFA and ImmunoCAP results were calculated. Results. The sensitivity/specificity of ALFA to the diagnosis was 100%/83% for BV and 82%/97% for WV. For insect venom allergic patients, the Spearman correlation coefficient for ALFA versus ImmunoCAP was 0.79 for BV and 0.80 for WV. However, significant differences in the negative control groups were observed. Conclusion. ALFA represents a simple, robust, and reliable tool for the rapid detection of sIgE to insect venoms.

No MeSH data available.


Receiver operating characteristic of ALFA and ALLERGO-LIQ for the diagnosis of bee (a) and wasp (b) venom allergy in monosensitized patients (group A) and control groups (C and D). Curve with dots indicates results for ALFA and curve with squares for ALLERGO-LIQ. Diagnosis of insect venom allergy was based on patient's history, skin testing, and detection of sIgE to bee or wasp venom by ImmunoCAP. Sensitivity/specificity for ALFA is 100%/83% (BV) and 82%/97% (WV) at a cut-off value of 10.0 RU and 100%/93% (BV) and 82%/93% (WV) for ALLERG-O-LIQ.
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fig2: Receiver operating characteristic of ALFA and ALLERGO-LIQ for the diagnosis of bee (a) and wasp (b) venom allergy in monosensitized patients (group A) and control groups (C and D). Curve with dots indicates results for ALFA and curve with squares for ALLERGO-LIQ. Diagnosis of insect venom allergy was based on patient's history, skin testing, and detection of sIgE to bee or wasp venom by ImmunoCAP. Sensitivity/specificity for ALFA is 100%/83% (BV) and 82%/97% (WV) at a cut-off value of 10.0 RU and 100%/93% (BV) and 82%/93% (WV) for ALLERG-O-LIQ.

Mentions: In monosensitized patients (group A only), 12/12 BV allergic patients and 23/28 WV allergic patients were positive in ALFA using a cut-off value for ALFA of 10.0 RU, corresponding to a sensitivity of 100% for BV and 82% for WV. Similar results were obtained by ALLERG-O-LIQ. In ALLERG-O-LIQ 12/12 BV allergic patients and 24/28 WV allergic patients were found positive (Figure 2).


Evaluation of a Novel Rapid Test System for the Detection of Specific IgE to Hymenoptera Venoms.

Pfender N, Lucassen R, Offermann N, Schulte-Pelkum J, Fooke M, Jakob T - J Allergy (Cairo) (2012)

Receiver operating characteristic of ALFA and ALLERGO-LIQ for the diagnosis of bee (a) and wasp (b) venom allergy in monosensitized patients (group A) and control groups (C and D). Curve with dots indicates results for ALFA and curve with squares for ALLERGO-LIQ. Diagnosis of insect venom allergy was based on patient's history, skin testing, and detection of sIgE to bee or wasp venom by ImmunoCAP. Sensitivity/specificity for ALFA is 100%/83% (BV) and 82%/97% (WV) at a cut-off value of 10.0 RU and 100%/93% (BV) and 82%/93% (WV) for ALLERG-O-LIQ.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3303699&req=5

fig2: Receiver operating characteristic of ALFA and ALLERGO-LIQ for the diagnosis of bee (a) and wasp (b) venom allergy in monosensitized patients (group A) and control groups (C and D). Curve with dots indicates results for ALFA and curve with squares for ALLERGO-LIQ. Diagnosis of insect venom allergy was based on patient's history, skin testing, and detection of sIgE to bee or wasp venom by ImmunoCAP. Sensitivity/specificity for ALFA is 100%/83% (BV) and 82%/97% (WV) at a cut-off value of 10.0 RU and 100%/93% (BV) and 82%/93% (WV) for ALLERG-O-LIQ.
Mentions: In monosensitized patients (group A only), 12/12 BV allergic patients and 23/28 WV allergic patients were positive in ALFA using a cut-off value for ALFA of 10.0 RU, corresponding to a sensitivity of 100% for BV and 82% for WV. Similar results were obtained by ALLERG-O-LIQ. In ALLERG-O-LIQ 12/12 BV allergic patients and 24/28 WV allergic patients were found positive (Figure 2).

Bottom Line: Sensitivity and specificity of ALFA and correlation of ALFA and ImmunoCAP results were calculated.Results.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Allergy Research Group, Department of Dermatology, University Medical Center Freiburg, Hauptstraße 7, 79104 Freiburg, Germany.

ABSTRACT
Background. The Allergy Lateral Flow Assay (ALFA) is a novel rapid assay for the detection of sIgE to allergens. The objective of this study is the evaluation of ALFA for the detection of sIgE to bee venom (BV) and wasp venom (WV) in insect venom allergic patients. Methods. Specific IgE to BV and WV was analyzed by ALFA, ALLERG-O-LIQ, and ImmunoCAP in 80 insect venom allergic patients and 60 control sera. Sensitivity and specificity of ALFA and correlation of ALFA and ImmunoCAP results were calculated. Results. The sensitivity/specificity of ALFA to the diagnosis was 100%/83% for BV and 82%/97% for WV. For insect venom allergic patients, the Spearman correlation coefficient for ALFA versus ImmunoCAP was 0.79 for BV and 0.80 for WV. However, significant differences in the negative control groups were observed. Conclusion. ALFA represents a simple, robust, and reliable tool for the rapid detection of sIgE to insect venoms.

No MeSH data available.