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AngiotensinII preconditioning promotes angiogenesis in vitro via ERKs phosphorylation.

Guan A, Zou Y, Gong H, Niu Y, Ye Y, Jia J, Li B, Zhang G, Qian S, Ge J - J. Biomed. Biotechnol. (2012)

Bottom Line: Incubation with AngII for 18 hrs significantly impaired the formation of capillary-like tubes comparing to that without AngII.CMVECs with AngII pretreatment for 5 and 10 min formed more capillary-like tubes than those without AngII pretreatment, suggesting that preconditioning with AngII at a lower dose for a short period could prevent the further damage of CMVECs by a higher concentration of AngII.In conclusion, preconditioning with a lower concentration of AngII for a short period prevents the subsequent impairment of CMVECs by a higher dose of AngII, at least in part, through the increase in ERKs phosphorylation.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China.

ABSTRACT
AngiotensinII (AngII) is involved in not only the formation of cardiac hypertrophy but also the development of cardiac remodeling both of which are associated with myocardial angiogenesis. This study was therefore performed to clarify the effects of AngII on the formation of vasculatures by cultured cardiac microvascular endothelial cells (CMVECs) after a long-period stimulation with or without the AngII preconditioning. Incubation with AngII for 18 hrs significantly impaired the formation of capillary-like tubes comparing to that without AngII. CMVECs with AngII pretreatment for 5 and 10 min formed more capillary-like tubes than those without AngII pretreatment, suggesting that preconditioning with AngII at a lower dose for a short period could prevent the further damage of CMVECs by a higher concentration of AngII. Moreover, AngII (10(-7) M) stimulation for 5 and 10 min significantly induced the increase in extracellular signal-regulated protein kinases (ERKs) phosphorylation, and an ERKs inhibitor, PD98059, abrogated the increase in the formation of capillary-like tubes induced by the AngII-pretreatment. In conclusion, preconditioning with a lower concentration of AngII for a short period prevents the subsequent impairment of CMVECs by a higher dose of AngII, at least in part, through the increase in ERKs phosphorylation.

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Time-dependent effects of AngII on ERKs phosphorylation in cultured CMVECs. CMVECs were treated with AngII (10−7 M) for the indicated times and then collected and lysed for western blot analysis. (a) Representative immunoblots showing the expression of phosphor ERKs. GAPDH was used as a loading control. (b) Quantitative analysis for the expression of phosphor ERKs. Data represent mean ± SD obtained from 6 independent experiments (n = 6);   ∗P < 0.05  versus control group (0 min).
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fig2: Time-dependent effects of AngII on ERKs phosphorylation in cultured CMVECs. CMVECs were treated with AngII (10−7 M) for the indicated times and then collected and lysed for western blot analysis. (a) Representative immunoblots showing the expression of phosphor ERKs. GAPDH was used as a loading control. (b) Quantitative analysis for the expression of phosphor ERKs. Data represent mean ± SD obtained from 6 independent experiments (n = 6);   ∗P < 0.05  versus control group (0 min).

Mentions: In order to identify the effect of AngII on ERKs phosphorylation in CMVECs, we observed levels of ERKs phosphorylation using western blotting analysis. The figures showed that ERKs phosphorylation in CMVECs under AngII (10−7 M) stimulation for 5 and 10 min was significantly increased more than those without AngII addition (Figures 2(a) and 2(b)).


AngiotensinII preconditioning promotes angiogenesis in vitro via ERKs phosphorylation.

Guan A, Zou Y, Gong H, Niu Y, Ye Y, Jia J, Li B, Zhang G, Qian S, Ge J - J. Biomed. Biotechnol. (2012)

Time-dependent effects of AngII on ERKs phosphorylation in cultured CMVECs. CMVECs were treated with AngII (10−7 M) for the indicated times and then collected and lysed for western blot analysis. (a) Representative immunoblots showing the expression of phosphor ERKs. GAPDH was used as a loading control. (b) Quantitative analysis for the expression of phosphor ERKs. Data represent mean ± SD obtained from 6 independent experiments (n = 6);   ∗P < 0.05  versus control group (0 min).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303689&req=5

fig2: Time-dependent effects of AngII on ERKs phosphorylation in cultured CMVECs. CMVECs were treated with AngII (10−7 M) for the indicated times and then collected and lysed for western blot analysis. (a) Representative immunoblots showing the expression of phosphor ERKs. GAPDH was used as a loading control. (b) Quantitative analysis for the expression of phosphor ERKs. Data represent mean ± SD obtained from 6 independent experiments (n = 6);   ∗P < 0.05  versus control group (0 min).
Mentions: In order to identify the effect of AngII on ERKs phosphorylation in CMVECs, we observed levels of ERKs phosphorylation using western blotting analysis. The figures showed that ERKs phosphorylation in CMVECs under AngII (10−7 M) stimulation for 5 and 10 min was significantly increased more than those without AngII addition (Figures 2(a) and 2(b)).

Bottom Line: Incubation with AngII for 18 hrs significantly impaired the formation of capillary-like tubes comparing to that without AngII.CMVECs with AngII pretreatment for 5 and 10 min formed more capillary-like tubes than those without AngII pretreatment, suggesting that preconditioning with AngII at a lower dose for a short period could prevent the further damage of CMVECs by a higher concentration of AngII.In conclusion, preconditioning with a lower concentration of AngII for a short period prevents the subsequent impairment of CMVECs by a higher dose of AngII, at least in part, through the increase in ERKs phosphorylation.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China.

ABSTRACT
AngiotensinII (AngII) is involved in not only the formation of cardiac hypertrophy but also the development of cardiac remodeling both of which are associated with myocardial angiogenesis. This study was therefore performed to clarify the effects of AngII on the formation of vasculatures by cultured cardiac microvascular endothelial cells (CMVECs) after a long-period stimulation with or without the AngII preconditioning. Incubation with AngII for 18 hrs significantly impaired the formation of capillary-like tubes comparing to that without AngII. CMVECs with AngII pretreatment for 5 and 10 min formed more capillary-like tubes than those without AngII pretreatment, suggesting that preconditioning with AngII at a lower dose for a short period could prevent the further damage of CMVECs by a higher concentration of AngII. Moreover, AngII (10(-7) M) stimulation for 5 and 10 min significantly induced the increase in extracellular signal-regulated protein kinases (ERKs) phosphorylation, and an ERKs inhibitor, PD98059, abrogated the increase in the formation of capillary-like tubes induced by the AngII-pretreatment. In conclusion, preconditioning with a lower concentration of AngII for a short period prevents the subsequent impairment of CMVECs by a higher dose of AngII, at least in part, through the increase in ERKs phosphorylation.

Show MeSH
Related in: MedlinePlus