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The anticaries effect of a food extract (shiitake) in a short-term clinical study.

Lingström P, Zaura E, Hassan H, Buijs MJ, Hedelin P, Pratten J, Spratt D, Daglia M, Karbowiak A, Signoretto C, Rosema M, van der Weijden F, Wilson M - J. Biomed. Biotechnol. (2012)

Bottom Line: Frequent rinses with shiitake reduced the metabolic activity of dental plaque.Minor differences in microbial composition between test sessions were found.To conclude, the results indicate that shiitake extract has anticariogenic potential, but not to the same extent as the positive control.

View Article: PubMed Central - PubMed

Affiliation: Department of Cariology, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. peter.lingstrom@odontologi.gu.se

ABSTRACT
The main objective was to investigate whether low-molecular-weight fraction of edible mushroom shiitake extract (Lentinus edodes) possesses caries-preventive properties. The study was designed as a double-blind, three-leg, cross-over, randomized, controlled clinical trial carried out on two series of volunteers at the University of Gothenburg, and the Academic Centre for Dentistry Amsterdam. Volunteers rinsed twice daily with a solution containing low-molecular-weight fraction of edible mushroom, placebo (negative control without active ingredients), or Meridol (positive control, AmF-SnF(2)) for two weeks, with a two-week washout period between each rinsing period. Changes in the acidogenicity of dental plaque before and after a sucrose challenge, shifts in microbial composition, and plaque scores were determined. Frequent rinses with shiitake reduced the metabolic activity of dental plaque. No reduction of plaque scores and no inhibition of the production of organic acids in plaque was found. Minor differences in microbial composition between test sessions were found. To conclude, the results indicate that shiitake extract has anticariogenic potential, but not to the same extent as the positive control.

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Related in: MedlinePlus

The changes in dental plaque pH up to 45 min after a mouth rinse with 10% sucrose for 1 min. The rinse was carried out after two weeks use of a mouth rinse with a shiitake mushroom extract, a placebo, or positive control (AmF-SnF2). Mean values for 30 subjects. The standard deviation for some of the time points is shown.
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Related In: Results  -  Collection


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fig1: The changes in dental plaque pH up to 45 min after a mouth rinse with 10% sucrose for 1 min. The rinse was carried out after two weeks use of a mouth rinse with a shiitake mushroom extract, a placebo, or positive control (AmF-SnF2). Mean values for 30 subjects. The standard deviation for some of the time points is shown.

Mentions: The most pronounced metabolic activity for the sucrose rinse at the end of the three test periods was found after rinsing with the placebo, and the least attenuated pH fall was found for the positive control (AmF-SnF2), while the active compound (shiitake) resulted in an intermediate position (Figure 1). A statistically significant difference when comparing the pH values at the different time points was found at 2 min between shiitake and placebo (P < 0.05). In the case of minimum pH, there was also a numerical difference between the three products, with a difference of 0.2 pH units between shiitake and placebo and the positive control, respectively (ns). Minor differences in plaque acidogenicity were found when evaluating the maximum pH decrease, as well as AUC5.7 and AUC6.2. Only minor numerical differences in plaque acidogenicity were found when comparing the results for the four washout periods (baseline and posttreatment; ns).


The anticaries effect of a food extract (shiitake) in a short-term clinical study.

Lingström P, Zaura E, Hassan H, Buijs MJ, Hedelin P, Pratten J, Spratt D, Daglia M, Karbowiak A, Signoretto C, Rosema M, van der Weijden F, Wilson M - J. Biomed. Biotechnol. (2012)

The changes in dental plaque pH up to 45 min after a mouth rinse with 10% sucrose for 1 min. The rinse was carried out after two weeks use of a mouth rinse with a shiitake mushroom extract, a placebo, or positive control (AmF-SnF2). Mean values for 30 subjects. The standard deviation for some of the time points is shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303676&req=5

fig1: The changes in dental plaque pH up to 45 min after a mouth rinse with 10% sucrose for 1 min. The rinse was carried out after two weeks use of a mouth rinse with a shiitake mushroom extract, a placebo, or positive control (AmF-SnF2). Mean values for 30 subjects. The standard deviation for some of the time points is shown.
Mentions: The most pronounced metabolic activity for the sucrose rinse at the end of the three test periods was found after rinsing with the placebo, and the least attenuated pH fall was found for the positive control (AmF-SnF2), while the active compound (shiitake) resulted in an intermediate position (Figure 1). A statistically significant difference when comparing the pH values at the different time points was found at 2 min between shiitake and placebo (P < 0.05). In the case of minimum pH, there was also a numerical difference between the three products, with a difference of 0.2 pH units between shiitake and placebo and the positive control, respectively (ns). Minor differences in plaque acidogenicity were found when evaluating the maximum pH decrease, as well as AUC5.7 and AUC6.2. Only minor numerical differences in plaque acidogenicity were found when comparing the results for the four washout periods (baseline and posttreatment; ns).

Bottom Line: Frequent rinses with shiitake reduced the metabolic activity of dental plaque.Minor differences in microbial composition between test sessions were found.To conclude, the results indicate that shiitake extract has anticariogenic potential, but not to the same extent as the positive control.

View Article: PubMed Central - PubMed

Affiliation: Department of Cariology, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. peter.lingstrom@odontologi.gu.se

ABSTRACT
The main objective was to investigate whether low-molecular-weight fraction of edible mushroom shiitake extract (Lentinus edodes) possesses caries-preventive properties. The study was designed as a double-blind, three-leg, cross-over, randomized, controlled clinical trial carried out on two series of volunteers at the University of Gothenburg, and the Academic Centre for Dentistry Amsterdam. Volunteers rinsed twice daily with a solution containing low-molecular-weight fraction of edible mushroom, placebo (negative control without active ingredients), or Meridol (positive control, AmF-SnF(2)) for two weeks, with a two-week washout period between each rinsing period. Changes in the acidogenicity of dental plaque before and after a sucrose challenge, shifts in microbial composition, and plaque scores were determined. Frequent rinses with shiitake reduced the metabolic activity of dental plaque. No reduction of plaque scores and no inhibition of the production of organic acids in plaque was found. Minor differences in microbial composition between test sessions were found. To conclude, the results indicate that shiitake extract has anticariogenic potential, but not to the same extent as the positive control.

Show MeSH
Related in: MedlinePlus