Limits...
Human blood-vessel-derived stem cells for tissue repair and regeneration.

Chen CW, Corselli M, Péault B, Huard J - J. Biomed. Biotechnol. (2012)

Bottom Line: Our group and other laboratories recently isolated multiple stem/progenitor cell subsets from blood vessels of adult human tissues.Each of the three structural layers of blood vessels: intima, media, and adventitia has been found to include at least one precursor population, that is, myogenic endothelial cells (MECs), pericytes, and adventitial cells (ACs), respectively.The applications of ACs in vascular remodeling and angiogenesis/vasculogenesis have been examined.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA. chc88@pitt.edu

ABSTRACT
Multipotent stem/progenitor cells with similar developmental potentials have been independently identified from diverse human tissue/organ cultures. The increasing recognition of the vascular/perivascular origin of mesenchymal precursors suggested blood vessels being a systemic source of adult stem/progenitor cells. Our group and other laboratories recently isolated multiple stem/progenitor cell subsets from blood vessels of adult human tissues. Each of the three structural layers of blood vessels: intima, media, and adventitia has been found to include at least one precursor population, that is, myogenic endothelial cells (MECs), pericytes, and adventitial cells (ACs), respectively. MECs and pericytes efficiently regenerate myofibers in injured and dystrophic skeletal muscles as well as improve cardiac function after myocardial infarction. The applications of ACs in vascular remodeling and angiogenesis/vasculogenesis have been examined. Our recent finding that MECs and pericytes can be purified from cryogenically banked human primary muscle cell culture further indicates their potential applications in personalized regenerative medicine.

Show MeSH

Related in: MedlinePlus

Schematic depiction of hBVSCs at the origin of mesenchymal stem/stromal cells (MSCs). (A) hBVSCs, including myogenic endothelial cells (MECs, red), pericytes (green), and adventitial cells (AC, blue), are dissociated from fresh muscle biopsy and separated from endothelial cells (yellow) and other cell types. (B) Dissociated cells are purified to homogeneity by fluorescence-activated cell sorting (FACS) and newly sorted MECs, pericytes, and ACs already exhibit multilineage developmental potentials. (C) FACS-purified pericytes, ACs, and possibly MECs give rise to authentic MSCs in long-term culture. (D) Nevertheless, whether native hBVSCs serve as a source of MSCs in situ and participate in tissue repair and regeneration remains an open question.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3303622&req=5

fig2: Schematic depiction of hBVSCs at the origin of mesenchymal stem/stromal cells (MSCs). (A) hBVSCs, including myogenic endothelial cells (MECs, red), pericytes (green), and adventitial cells (AC, blue), are dissociated from fresh muscle biopsy and separated from endothelial cells (yellow) and other cell types. (B) Dissociated cells are purified to homogeneity by fluorescence-activated cell sorting (FACS) and newly sorted MECs, pericytes, and ACs already exhibit multilineage developmental potentials. (C) FACS-purified pericytes, ACs, and possibly MECs give rise to authentic MSCs in long-term culture. (D) Nevertheless, whether native hBVSCs serve as a source of MSCs in situ and participate in tissue repair and regeneration remains an open question.

Mentions: Nevertheless, while all, or at least part of, the three hBVSC subpopulations contributing to the MSC entity in culture are gradually becoming an accepted notion; whether the multilineage potentials are natively present within hBVSC subsets and subsequently responsive to pathological stimulations in vivo remains to be investigated (Figure 2). Ultimately, the current therapeutic strategy based on the transplantation of unfractionated stromal cells may in the near future be replaced by the purification, combination, and direct reinfusion of the distinct subsets of hBVSCs, devoid of cells with none or a restricted regenerative potential.


Human blood-vessel-derived stem cells for tissue repair and regeneration.

Chen CW, Corselli M, Péault B, Huard J - J. Biomed. Biotechnol. (2012)

Schematic depiction of hBVSCs at the origin of mesenchymal stem/stromal cells (MSCs). (A) hBVSCs, including myogenic endothelial cells (MECs, red), pericytes (green), and adventitial cells (AC, blue), are dissociated from fresh muscle biopsy and separated from endothelial cells (yellow) and other cell types. (B) Dissociated cells are purified to homogeneity by fluorescence-activated cell sorting (FACS) and newly sorted MECs, pericytes, and ACs already exhibit multilineage developmental potentials. (C) FACS-purified pericytes, ACs, and possibly MECs give rise to authentic MSCs in long-term culture. (D) Nevertheless, whether native hBVSCs serve as a source of MSCs in situ and participate in tissue repair and regeneration remains an open question.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303622&req=5

fig2: Schematic depiction of hBVSCs at the origin of mesenchymal stem/stromal cells (MSCs). (A) hBVSCs, including myogenic endothelial cells (MECs, red), pericytes (green), and adventitial cells (AC, blue), are dissociated from fresh muscle biopsy and separated from endothelial cells (yellow) and other cell types. (B) Dissociated cells are purified to homogeneity by fluorescence-activated cell sorting (FACS) and newly sorted MECs, pericytes, and ACs already exhibit multilineage developmental potentials. (C) FACS-purified pericytes, ACs, and possibly MECs give rise to authentic MSCs in long-term culture. (D) Nevertheless, whether native hBVSCs serve as a source of MSCs in situ and participate in tissue repair and regeneration remains an open question.
Mentions: Nevertheless, while all, or at least part of, the three hBVSC subpopulations contributing to the MSC entity in culture are gradually becoming an accepted notion; whether the multilineage potentials are natively present within hBVSC subsets and subsequently responsive to pathological stimulations in vivo remains to be investigated (Figure 2). Ultimately, the current therapeutic strategy based on the transplantation of unfractionated stromal cells may in the near future be replaced by the purification, combination, and direct reinfusion of the distinct subsets of hBVSCs, devoid of cells with none or a restricted regenerative potential.

Bottom Line: Our group and other laboratories recently isolated multiple stem/progenitor cell subsets from blood vessels of adult human tissues.Each of the three structural layers of blood vessels: intima, media, and adventitia has been found to include at least one precursor population, that is, myogenic endothelial cells (MECs), pericytes, and adventitial cells (ACs), respectively.The applications of ACs in vascular remodeling and angiogenesis/vasculogenesis have been examined.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA. chc88@pitt.edu

ABSTRACT
Multipotent stem/progenitor cells with similar developmental potentials have been independently identified from diverse human tissue/organ cultures. The increasing recognition of the vascular/perivascular origin of mesenchymal precursors suggested blood vessels being a systemic source of adult stem/progenitor cells. Our group and other laboratories recently isolated multiple stem/progenitor cell subsets from blood vessels of adult human tissues. Each of the three structural layers of blood vessels: intima, media, and adventitia has been found to include at least one precursor population, that is, myogenic endothelial cells (MECs), pericytes, and adventitial cells (ACs), respectively. MECs and pericytes efficiently regenerate myofibers in injured and dystrophic skeletal muscles as well as improve cardiac function after myocardial infarction. The applications of ACs in vascular remodeling and angiogenesis/vasculogenesis have been examined. Our recent finding that MECs and pericytes can be purified from cryogenically banked human primary muscle cell culture further indicates their potential applications in personalized regenerative medicine.

Show MeSH
Related in: MedlinePlus