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Sesquiterpene lactones isolated from Elephantopus scaber L. inhibits human lymphocyte proliferation and the growth of tumour cell lines and induces apoptosis in vitro.

Geetha BS, Nair MS, Latha PG, Remani P - J. Biomed. Biotechnol. (2012)

Bottom Line: Bioassay-guided isolation of the whole plant of chloroform extract of South Indian Elephantopus scaber afforded the known sesquiterpene lactone, deoxyelephantopin, and isodeoxyelephantopin whose structures were determined by spectroscopic methods.These compounds caused a dose dependent reduction in the viability of L-929 tumour cells in 72 h culture (IC(50) value of 2.7 μg/mL and 3.3 μg/mL) by the cell viability assay.The compound deoxyelephantopin at a concentration of 3 μg/mL caused maximum apoptotic cells.

View Article: PubMed Central - PubMed

Affiliation: Phytochemistry and Phytopharmacology Division, Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram, India. dayal.geetha@gmail.com

ABSTRACT
This study was designed to isolate the compounds responsible for the cytotoxic properties of South Indian Elephantopus scaber L. and further investigate their effects on quiescent and proliferating cells. Bioassay-guided isolation of the whole plant of chloroform extract of South Indian Elephantopus scaber afforded the known sesquiterpene lactone, deoxyelephantopin, and isodeoxyelephantopin whose structures were determined by spectroscopic methods. These compounds caused a dose dependent reduction in the viability of L-929 tumour cells in 72 h culture (IC(50) value of 2.7 μg/mL and 3.3 μg/mL) by the cell viability assay. Both the compounds act selectively on quiescent and PHA-stimulated proliferating human lymphocytes and inhibited tritiated thymidine incorporation into cellular DNA of DLA tumour cells. The compound deoxyelephantopin at a concentration of 3 μg/mL caused maximum apoptotic cells. It also exhibited significant in vivo antitumour efficacy against DLA tumour cells. The results, therefore, indicate that the antiproliferative property of deoxyelephantopin and isodeoxyelephantopin could be used in regimens for treating tumors with extensive proliferative potencies.

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Effect of deoxyelephantopin and isodeoxyelephantopin on percentage inhibition of DNA synthesis of DLA tumour cells.
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Related In: Results  -  Collection


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fig6: Effect of deoxyelephantopin and isodeoxyelephantopin on percentage inhibition of DNA synthesis of DLA tumour cells.

Mentions: DLA cells treated both with deoxyelephantopin and isodeoxyelephantopin showed decreased [3H] thymidine incorporation into the nuclear DNA, suggesting that they inhibited DNA synthesis. The results on DNA synthesis also indicated a concentration-dependent decrease in [3H] thymidine incorporation (Figure 5). The concentration of deoxyelephantopin and isodeoxyelephantopin required to produce 50% in-incorporation was 2.2 μg/mL and 2.9 μg/mL, respectively (Figure 6).


Sesquiterpene lactones isolated from Elephantopus scaber L. inhibits human lymphocyte proliferation and the growth of tumour cell lines and induces apoptosis in vitro.

Geetha BS, Nair MS, Latha PG, Remani P - J. Biomed. Biotechnol. (2012)

Effect of deoxyelephantopin and isodeoxyelephantopin on percentage inhibition of DNA synthesis of DLA tumour cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303583&req=5

fig6: Effect of deoxyelephantopin and isodeoxyelephantopin on percentage inhibition of DNA synthesis of DLA tumour cells.
Mentions: DLA cells treated both with deoxyelephantopin and isodeoxyelephantopin showed decreased [3H] thymidine incorporation into the nuclear DNA, suggesting that they inhibited DNA synthesis. The results on DNA synthesis also indicated a concentration-dependent decrease in [3H] thymidine incorporation (Figure 5). The concentration of deoxyelephantopin and isodeoxyelephantopin required to produce 50% in-incorporation was 2.2 μg/mL and 2.9 μg/mL, respectively (Figure 6).

Bottom Line: Bioassay-guided isolation of the whole plant of chloroform extract of South Indian Elephantopus scaber afforded the known sesquiterpene lactone, deoxyelephantopin, and isodeoxyelephantopin whose structures were determined by spectroscopic methods.These compounds caused a dose dependent reduction in the viability of L-929 tumour cells in 72 h culture (IC(50) value of 2.7 μg/mL and 3.3 μg/mL) by the cell viability assay.The compound deoxyelephantopin at a concentration of 3 μg/mL caused maximum apoptotic cells.

View Article: PubMed Central - PubMed

Affiliation: Phytochemistry and Phytopharmacology Division, Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram, India. dayal.geetha@gmail.com

ABSTRACT
This study was designed to isolate the compounds responsible for the cytotoxic properties of South Indian Elephantopus scaber L. and further investigate their effects on quiescent and proliferating cells. Bioassay-guided isolation of the whole plant of chloroform extract of South Indian Elephantopus scaber afforded the known sesquiterpene lactone, deoxyelephantopin, and isodeoxyelephantopin whose structures were determined by spectroscopic methods. These compounds caused a dose dependent reduction in the viability of L-929 tumour cells in 72 h culture (IC(50) value of 2.7 μg/mL and 3.3 μg/mL) by the cell viability assay. Both the compounds act selectively on quiescent and PHA-stimulated proliferating human lymphocytes and inhibited tritiated thymidine incorporation into cellular DNA of DLA tumour cells. The compound deoxyelephantopin at a concentration of 3 μg/mL caused maximum apoptotic cells. It also exhibited significant in vivo antitumour efficacy against DLA tumour cells. The results, therefore, indicate that the antiproliferative property of deoxyelephantopin and isodeoxyelephantopin could be used in regimens for treating tumors with extensive proliferative potencies.

Show MeSH
Related in: MedlinePlus