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Oropharyngeal candidosis relative frequency in radiotherapy patient for head and neck cancer.

Suryawanshi H, Ganvir SM, Hazarey VK, Wanjare VS - J Oral Maxillofac Pathol (2012)

Bottom Line: Sabourauds Dextrose Agar (SDA) was used as primary culture media and subsequently speciation was done using standard techniques.The occurrence of erythmatous lesions, ulceration, and xerostomia were found to be statistically significant (P<0.05).C. albicans was the most frequently encountered species with higher prevalence of serotype A suggesting higher virulent species.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Pathology, V.S.P.M.'s Dental College and Research Centre, Hingna, India.

ABSTRACT

Background: Radiation given during treatment of oral and pharyngeal malignancy frequently causes alteration of the oral environment predisposing to the colonization of the oral mucosa by yeast species most frequently Candida.

Objective: Thus, this study was undertaken in 107 patients to find out association between radiation therapy and frequency of oropharyngeal candidosis, to quantitate colony forming units (CFUs) to identify Candida at species level and to check the incidence of serotype A and B in C. albicans.

Materials and methods: The study was done on patients suffering from oropharyngeal cancer who were advised radiotherapy. The oral rinse collection method was used to collect the sample. Sabourauds Dextrose Agar (SDA) was used as primary culture media and subsequently speciation was done using standard techniques. The strains of C. albicans were serotyped employing the method described by Hansclever and Mitchell (1961, J Bacteriol 1961;82:570-3).

Results: 26.16% patients were mycologically positive for candida before radiotherapy with CFUs 100. 14 ± 59.11 that increased to 60.74% patients during radiotherapy with an increase in CFUs to 490.15 ± 207.97. Clinically, grading of mucositis was done and also individual signs and symptoms were noted in each patient. The occurrence of erythmatous lesions, ulceration, and xerostomia were found to be statistically significant (P<0.05). C. albicans was the most frequently encountered species with higher prevalence of serotype A suggesting higher virulent species.

Conclusion: It is proposed that in such patients taking radiotherapy prophylactic antifungal treatment should be given specially in patients showing development of oral mucosal lesions such as erythmatous lesions, ulcerations, and complaining about dryness of mouth, that is, xerostomia irrespective of presence or absence of clinical oral candidosis.

No MeSH data available.


Related in: MedlinePlus

Photomicrograph showing pseudo hyphae with branching of C. krusei on corn meal Tween 80 agar
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Figure 7: Photomicrograph showing pseudo hyphae with branching of C. krusei on corn meal Tween 80 agar

Mentions: Out of 107 patients 28 patients (26.16%) showed positive oral cultures at first visit. The number of CFUs/0.1 mL of each patient was noted. The range and mean value±standard deviation of CFUs/0.1 mL was 100.14 ± 59.11. Serotyping of 22 confirmed strains of C. albicans showed that 19 strains reacted quickly with A specific antiserum forming large clumps [Figure 2]. The remaining three strains however failed to agglutinate with A specific antiserum that were of serotype B. Identification of 28 isolates at species level showed that C. albicans was seen in 78.57%, C. krusei- 7.15%, and C. tropicalis, C. guilliermondii, C. stellatoidea, and C. parapsilosis each being 3.57% [Figures 3–7].


Oropharyngeal candidosis relative frequency in radiotherapy patient for head and neck cancer.

Suryawanshi H, Ganvir SM, Hazarey VK, Wanjare VS - J Oral Maxillofac Pathol (2012)

Photomicrograph showing pseudo hyphae with branching of C. krusei on corn meal Tween 80 agar
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3303519&req=5

Figure 7: Photomicrograph showing pseudo hyphae with branching of C. krusei on corn meal Tween 80 agar
Mentions: Out of 107 patients 28 patients (26.16%) showed positive oral cultures at first visit. The number of CFUs/0.1 mL of each patient was noted. The range and mean value±standard deviation of CFUs/0.1 mL was 100.14 ± 59.11. Serotyping of 22 confirmed strains of C. albicans showed that 19 strains reacted quickly with A specific antiserum forming large clumps [Figure 2]. The remaining three strains however failed to agglutinate with A specific antiserum that were of serotype B. Identification of 28 isolates at species level showed that C. albicans was seen in 78.57%, C. krusei- 7.15%, and C. tropicalis, C. guilliermondii, C. stellatoidea, and C. parapsilosis each being 3.57% [Figures 3–7].

Bottom Line: Sabourauds Dextrose Agar (SDA) was used as primary culture media and subsequently speciation was done using standard techniques.The occurrence of erythmatous lesions, ulceration, and xerostomia were found to be statistically significant (P<0.05).C. albicans was the most frequently encountered species with higher prevalence of serotype A suggesting higher virulent species.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Pathology, V.S.P.M.'s Dental College and Research Centre, Hingna, India.

ABSTRACT

Background: Radiation given during treatment of oral and pharyngeal malignancy frequently causes alteration of the oral environment predisposing to the colonization of the oral mucosa by yeast species most frequently Candida.

Objective: Thus, this study was undertaken in 107 patients to find out association between radiation therapy and frequency of oropharyngeal candidosis, to quantitate colony forming units (CFUs) to identify Candida at species level and to check the incidence of serotype A and B in C. albicans.

Materials and methods: The study was done on patients suffering from oropharyngeal cancer who were advised radiotherapy. The oral rinse collection method was used to collect the sample. Sabourauds Dextrose Agar (SDA) was used as primary culture media and subsequently speciation was done using standard techniques. The strains of C. albicans were serotyped employing the method described by Hansclever and Mitchell (1961, J Bacteriol 1961;82:570-3).

Results: 26.16% patients were mycologically positive for candida before radiotherapy with CFUs 100. 14 ± 59.11 that increased to 60.74% patients during radiotherapy with an increase in CFUs to 490.15 ± 207.97. Clinically, grading of mucositis was done and also individual signs and symptoms were noted in each patient. The occurrence of erythmatous lesions, ulceration, and xerostomia were found to be statistically significant (P<0.05). C. albicans was the most frequently encountered species with higher prevalence of serotype A suggesting higher virulent species.

Conclusion: It is proposed that in such patients taking radiotherapy prophylactic antifungal treatment should be given specially in patients showing development of oral mucosal lesions such as erythmatous lesions, ulcerations, and complaining about dryness of mouth, that is, xerostomia irrespective of presence or absence of clinical oral candidosis.

No MeSH data available.


Related in: MedlinePlus