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Assessment of the innervation pattern of oral squamous cell carcinoma using neural protein gene product (9.5)-An immunocytochemical study.

Habash FS, Hantash RO, Yunis MA - J Oral Maxillofac Pathol (2012)

Bottom Line: PGP 9.5 was used to localize nerve fibers in oral squamous cell carcinoma.There was no PGP9.5 immunoreactivity in the normal tissue adjacent to the tumor in 18 cases out of 30 (60%).Labeled nerve fibers between tumor cells were detected only in 2 out of the 30 cases.

View Article: PubMed Central - PubMed

Affiliation: Head Department of Oral Surgery, Al Quds University, Jerusalem, Palestine.

ABSTRACT

Background: This study was carried out to assess the innervation patterns in oral cancer using the general neuronal marker protein gene product 9.5 (PGP 9.5) and to find an explanation for why oral cancer tends to be painless in the early stages.

Materials and methods: Tumor tissue from 30 unselected patients with oral squamous cell carcinoma was evaluated for this study. PGP 9.5 was used to localize nerve fibers in oral squamous cell carcinoma. An indirect immunofluorescence technique using biotin/FITC streptavidin detection system was used on paraffin wax sections of tumor tissue fixed in neutral buffered formalin.

Results: There was no PGP9.5 immunoreactivity in the normal tissue adjacent to the tumor in 18 cases out of 30 (60%). In 12/30 of oral squamous cell carcinoma, preexisting nerve fibers were detected in tissue stroma adjacent to the cancer tissue. Labeled nerve fibers between tumor cells were detected only in 2 out of the 30 cases.

Conclusion: There are no apparent patterns of innervations in the cancer tissues themselves, but there are innervations patterns in the surrounding tissue, which presumably represents preexisting nerves. These results may partly explain why oral cancer tends to be painless in the early stages.

No MeSH data available.


Related in: MedlinePlus

A large number of PGP 9.5-immunoreactive nerve fibers passing through the tumor tissue between the tumor cells in an oral squamous cell carcinoma (original magnification ×240)
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Figure 3: A large number of PGP 9.5-immunoreactive nerve fibers passing through the tumor tissue between the tumor cells in an oral squamous cell carcinoma (original magnification ×240)

Mentions: The tumor tissue itself, however, was not apparently innervated. PGP9.5-labeled nerve fibers were detected only in 2 cases out of 30 (6.6%) in the tumor tissue itself [Figure 3] One case (stage: TINO, grade 2; site: Floor of the mouth anteriorly) was interesting because it had a fair number of nerve fibers passing between the tumor cells. PGP9.5-immunoreactive fine nerve fibers were seen in the affected area of the tissue samples of this case [Figure 4]. It was the only tumor examined in which it could be concluded that there was an innervation pattern in the tumor tissue itself.


Assessment of the innervation pattern of oral squamous cell carcinoma using neural protein gene product (9.5)-An immunocytochemical study.

Habash FS, Hantash RO, Yunis MA - J Oral Maxillofac Pathol (2012)

A large number of PGP 9.5-immunoreactive nerve fibers passing through the tumor tissue between the tumor cells in an oral squamous cell carcinoma (original magnification ×240)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3303516&req=5

Figure 3: A large number of PGP 9.5-immunoreactive nerve fibers passing through the tumor tissue between the tumor cells in an oral squamous cell carcinoma (original magnification ×240)
Mentions: The tumor tissue itself, however, was not apparently innervated. PGP9.5-labeled nerve fibers were detected only in 2 cases out of 30 (6.6%) in the tumor tissue itself [Figure 3] One case (stage: TINO, grade 2; site: Floor of the mouth anteriorly) was interesting because it had a fair number of nerve fibers passing between the tumor cells. PGP9.5-immunoreactive fine nerve fibers were seen in the affected area of the tissue samples of this case [Figure 4]. It was the only tumor examined in which it could be concluded that there was an innervation pattern in the tumor tissue itself.

Bottom Line: PGP 9.5 was used to localize nerve fibers in oral squamous cell carcinoma.There was no PGP9.5 immunoreactivity in the normal tissue adjacent to the tumor in 18 cases out of 30 (60%).Labeled nerve fibers between tumor cells were detected only in 2 out of the 30 cases.

View Article: PubMed Central - PubMed

Affiliation: Head Department of Oral Surgery, Al Quds University, Jerusalem, Palestine.

ABSTRACT

Background: This study was carried out to assess the innervation patterns in oral cancer using the general neuronal marker protein gene product 9.5 (PGP 9.5) and to find an explanation for why oral cancer tends to be painless in the early stages.

Materials and methods: Tumor tissue from 30 unselected patients with oral squamous cell carcinoma was evaluated for this study. PGP 9.5 was used to localize nerve fibers in oral squamous cell carcinoma. An indirect immunofluorescence technique using biotin/FITC streptavidin detection system was used on paraffin wax sections of tumor tissue fixed in neutral buffered formalin.

Results: There was no PGP9.5 immunoreactivity in the normal tissue adjacent to the tumor in 18 cases out of 30 (60%). In 12/30 of oral squamous cell carcinoma, preexisting nerve fibers were detected in tissue stroma adjacent to the cancer tissue. Labeled nerve fibers between tumor cells were detected only in 2 out of the 30 cases.

Conclusion: There are no apparent patterns of innervations in the cancer tissues themselves, but there are innervations patterns in the surrounding tissue, which presumably represents preexisting nerves. These results may partly explain why oral cancer tends to be painless in the early stages.

No MeSH data available.


Related in: MedlinePlus