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Protective effect of theaflavins on neuron against 6-hydroxydopamine-induced apoptosis in SH-SY5Y cells.

Luo Z, Zhao Y, Wang Y, Yang X, Zhao B - J Clin Biochem Nutr (2011)

Bottom Line: Here we investigated the protective effect of theaflavins on 6-hydroxydopamine induced SH-SY5Y cells against apoptosis (within this concentration range).It was found that pretreating SH-SY5Y cells with 0.5 µg/ml of theaflavins prevented 6-hydroxydopamine-induced loss of cell viability, condensed nuclear morphology, attenuated 6-hydroxydopamine-induced apoptosis, decrease of mitochondrial membrane potential and the increase of intracellular nitric oxide levels.Our results indicated that theaflavins had protective effect against 6-hydroxydopamine induced apoptosis at low concentrations, possibly through inhibition of reactive oxygen species and nitric oxide production.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang University Tea Research Institute, 268 Kaixuan Road, Hangzhou 310029, P.R. China.

ABSTRACT
Theaflavins, the oxidation products of tea polyphenols are important biologically active components of black tea. 6-hydroxydopamine is a pro-parkinsonian neurotoxin. Theaflavins could inhibit the auto-oxidation of 6-hydroxydopamine in a dose-dependent manner from 0.5 µg/ml to 25 µg/ml. Here we investigated the protective effect of theaflavins on 6-hydroxydopamine induced SH-SY5Y cells against apoptosis (within this concentration range). It was found that pretreating SH-SY5Y cells with 0.5 µg/ml of theaflavins prevented 6-hydroxydopamine-induced loss of cell viability, condensed nuclear morphology, attenuated 6-hydroxydopamine-induced apoptosis, decrease of mitochondrial membrane potential and the increase of intracellular nitric oxide levels. Our results indicated that theaflavins had protective effect against 6-hydroxydopamine induced apoptosis at low concentrations, possibly through inhibition of reactive oxygen species and nitric oxide production.

No MeSH data available.


The constituents of TFs detected by HPLC.
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Figure 7: The constituents of TFs detected by HPLC.

Mentions: The result (Fig. 7) showed that the purity of our TFs is 81.89%, containing TF, TF-3-G, TF-3'-G and TFDG (the peak of retention time at 8 min in Fig. 7 is EGCG, one monomer of green tea polyphenols). Like EGCG that is considered as the main active component of green tea polyphenols, there may be a monomer in TFs which is the main active component against neuronal cell death. We are in process to separate the monomers from TFs, and may find out which monomer is the most efficient for PD prevention in the future.


Protective effect of theaflavins on neuron against 6-hydroxydopamine-induced apoptosis in SH-SY5Y cells.

Luo Z, Zhao Y, Wang Y, Yang X, Zhao B - J Clin Biochem Nutr (2011)

The constituents of TFs detected by HPLC.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303475&req=5

Figure 7: The constituents of TFs detected by HPLC.
Mentions: The result (Fig. 7) showed that the purity of our TFs is 81.89%, containing TF, TF-3-G, TF-3'-G and TFDG (the peak of retention time at 8 min in Fig. 7 is EGCG, one monomer of green tea polyphenols). Like EGCG that is considered as the main active component of green tea polyphenols, there may be a monomer in TFs which is the main active component against neuronal cell death. We are in process to separate the monomers from TFs, and may find out which monomer is the most efficient for PD prevention in the future.

Bottom Line: Here we investigated the protective effect of theaflavins on 6-hydroxydopamine induced SH-SY5Y cells against apoptosis (within this concentration range).It was found that pretreating SH-SY5Y cells with 0.5 µg/ml of theaflavins prevented 6-hydroxydopamine-induced loss of cell viability, condensed nuclear morphology, attenuated 6-hydroxydopamine-induced apoptosis, decrease of mitochondrial membrane potential and the increase of intracellular nitric oxide levels.Our results indicated that theaflavins had protective effect against 6-hydroxydopamine induced apoptosis at low concentrations, possibly through inhibition of reactive oxygen species and nitric oxide production.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang University Tea Research Institute, 268 Kaixuan Road, Hangzhou 310029, P.R. China.

ABSTRACT
Theaflavins, the oxidation products of tea polyphenols are important biologically active components of black tea. 6-hydroxydopamine is a pro-parkinsonian neurotoxin. Theaflavins could inhibit the auto-oxidation of 6-hydroxydopamine in a dose-dependent manner from 0.5 µg/ml to 25 µg/ml. Here we investigated the protective effect of theaflavins on 6-hydroxydopamine induced SH-SY5Y cells against apoptosis (within this concentration range). It was found that pretreating SH-SY5Y cells with 0.5 µg/ml of theaflavins prevented 6-hydroxydopamine-induced loss of cell viability, condensed nuclear morphology, attenuated 6-hydroxydopamine-induced apoptosis, decrease of mitochondrial membrane potential and the increase of intracellular nitric oxide levels. Our results indicated that theaflavins had protective effect against 6-hydroxydopamine induced apoptosis at low concentrations, possibly through inhibition of reactive oxygen species and nitric oxide production.

No MeSH data available.