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Protective effect of theaflavins on neuron against 6-hydroxydopamine-induced apoptosis in SH-SY5Y cells.

Luo Z, Zhao Y, Wang Y, Yang X, Zhao B - J Clin Biochem Nutr (2011)

Bottom Line: Here we investigated the protective effect of theaflavins on 6-hydroxydopamine induced SH-SY5Y cells against apoptosis (within this concentration range).It was found that pretreating SH-SY5Y cells with 0.5 µg/ml of theaflavins prevented 6-hydroxydopamine-induced loss of cell viability, condensed nuclear morphology, attenuated 6-hydroxydopamine-induced apoptosis, decrease of mitochondrial membrane potential and the increase of intracellular nitric oxide levels.Our results indicated that theaflavins had protective effect against 6-hydroxydopamine induced apoptosis at low concentrations, possibly through inhibition of reactive oxygen species and nitric oxide production.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang University Tea Research Institute, 268 Kaixuan Road, Hangzhou 310029, P.R. China.

ABSTRACT
Theaflavins, the oxidation products of tea polyphenols are important biologically active components of black tea. 6-hydroxydopamine is a pro-parkinsonian neurotoxin. Theaflavins could inhibit the auto-oxidation of 6-hydroxydopamine in a dose-dependent manner from 0.5 µg/ml to 25 µg/ml. Here we investigated the protective effect of theaflavins on 6-hydroxydopamine induced SH-SY5Y cells against apoptosis (within this concentration range). It was found that pretreating SH-SY5Y cells with 0.5 µg/ml of theaflavins prevented 6-hydroxydopamine-induced loss of cell viability, condensed nuclear morphology, attenuated 6-hydroxydopamine-induced apoptosis, decrease of mitochondrial membrane potential and the increase of intracellular nitric oxide levels. Our results indicated that theaflavins had protective effect against 6-hydroxydopamine induced apoptosis at low concentrations, possibly through inhibition of reactive oxygen species and nitric oxide production.

No MeSH data available.


TFs attenuate 6-OHDA-induced decrease of mitochondrial membrane potential. Cells exposed to 6-OHDA were incubated with rhodamine 123, and the fluorescence intensity was measured. MMP of control is defined as 100% and the data are expressed as percentage of the untreated control means ± SD, n = 5. *p<0.01 significantly different from untreated control cells and **p<0.01 significantly different from 6-OHDA group.
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Figure 5: TFs attenuate 6-OHDA-induced decrease of mitochondrial membrane potential. Cells exposed to 6-OHDA were incubated with rhodamine 123, and the fluorescence intensity was measured. MMP of control is defined as 100% and the data are expressed as percentage of the untreated control means ± SD, n = 5. *p<0.01 significantly different from untreated control cells and **p<0.01 significantly different from 6-OHDA group.

Mentions: After SH-SY5Y cells were exposed to 6-OHDA for 24 h, the fluorescent intensity of rhodamine 123 staining of the cells was decreased, representing a fall in the mitochondrial membrane potential. Since low concentrations of TFs (0.5–25 µg/ml) protected cells against 6-OHDA toxicity, we tested whether pre-treatments of TFs (0.5 µg/ml, 1 µg/ml, 2 µg/ml) before 100 µM 6-OHDA treatment affected mitochondrial membrane potential. The results showed that TFs at this concentrations have significantly inhibited the fall of mitochondrial membrane potential caused by 6-OHDA at 100 µM (Fig. 5).


Protective effect of theaflavins on neuron against 6-hydroxydopamine-induced apoptosis in SH-SY5Y cells.

Luo Z, Zhao Y, Wang Y, Yang X, Zhao B - J Clin Biochem Nutr (2011)

TFs attenuate 6-OHDA-induced decrease of mitochondrial membrane potential. Cells exposed to 6-OHDA were incubated with rhodamine 123, and the fluorescence intensity was measured. MMP of control is defined as 100% and the data are expressed as percentage of the untreated control means ± SD, n = 5. *p<0.01 significantly different from untreated control cells and **p<0.01 significantly different from 6-OHDA group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303475&req=5

Figure 5: TFs attenuate 6-OHDA-induced decrease of mitochondrial membrane potential. Cells exposed to 6-OHDA were incubated with rhodamine 123, and the fluorescence intensity was measured. MMP of control is defined as 100% and the data are expressed as percentage of the untreated control means ± SD, n = 5. *p<0.01 significantly different from untreated control cells and **p<0.01 significantly different from 6-OHDA group.
Mentions: After SH-SY5Y cells were exposed to 6-OHDA for 24 h, the fluorescent intensity of rhodamine 123 staining of the cells was decreased, representing a fall in the mitochondrial membrane potential. Since low concentrations of TFs (0.5–25 µg/ml) protected cells against 6-OHDA toxicity, we tested whether pre-treatments of TFs (0.5 µg/ml, 1 µg/ml, 2 µg/ml) before 100 µM 6-OHDA treatment affected mitochondrial membrane potential. The results showed that TFs at this concentrations have significantly inhibited the fall of mitochondrial membrane potential caused by 6-OHDA at 100 µM (Fig. 5).

Bottom Line: Here we investigated the protective effect of theaflavins on 6-hydroxydopamine induced SH-SY5Y cells against apoptosis (within this concentration range).It was found that pretreating SH-SY5Y cells with 0.5 µg/ml of theaflavins prevented 6-hydroxydopamine-induced loss of cell viability, condensed nuclear morphology, attenuated 6-hydroxydopamine-induced apoptosis, decrease of mitochondrial membrane potential and the increase of intracellular nitric oxide levels.Our results indicated that theaflavins had protective effect against 6-hydroxydopamine induced apoptosis at low concentrations, possibly through inhibition of reactive oxygen species and nitric oxide production.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang University Tea Research Institute, 268 Kaixuan Road, Hangzhou 310029, P.R. China.

ABSTRACT
Theaflavins, the oxidation products of tea polyphenols are important biologically active components of black tea. 6-hydroxydopamine is a pro-parkinsonian neurotoxin. Theaflavins could inhibit the auto-oxidation of 6-hydroxydopamine in a dose-dependent manner from 0.5 µg/ml to 25 µg/ml. Here we investigated the protective effect of theaflavins on 6-hydroxydopamine induced SH-SY5Y cells against apoptosis (within this concentration range). It was found that pretreating SH-SY5Y cells with 0.5 µg/ml of theaflavins prevented 6-hydroxydopamine-induced loss of cell viability, condensed nuclear morphology, attenuated 6-hydroxydopamine-induced apoptosis, decrease of mitochondrial membrane potential and the increase of intracellular nitric oxide levels. Our results indicated that theaflavins had protective effect against 6-hydroxydopamine induced apoptosis at low concentrations, possibly through inhibition of reactive oxygen species and nitric oxide production.

No MeSH data available.