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Separation of cis- and trans-Asarone from Acorus tatarinowii by Preparative Gas Chromatography.

Zuo HL, Yang FQ, Zhang XM, Xia ZN - J Anal Methods Chem (2012)

Bottom Line: And then the fraction that contains mixture of the isomers was further separated by pGC.The compounds were separated on a stainless steel column packed with 10% OV-101 (3 m × 6 mm, i.d.), and then the effluent was split into two gas flows.Two isomers were collected after 90 single injections (5 uL) with the yield of 178 mg and 82 mg, respectively.

View Article: PubMed Central - PubMed

Affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China.

ABSTRACT
A preparative gas chromatography (pGC) method was developed for the separation of isomers (cis- and trans-asarone) from essential oil of Acorus tatarinowii. The oil was primarily fractionated by silica gel chromatography using different ratios of petroleum ether and ethyl acetate as gradient elution solvents. And then the fraction that contains mixture of the isomers was further separated by pGC. The compounds were separated on a stainless steel column packed with 10% OV-101 (3 m × 6 mm, i.d.), and then the effluent was split into two gas flows. One percent of the effluent passed to the flame ionization detector (FID) for detection and the remaining 99% was directed to the fraction collector. Two isomers were collected after 90 single injections (5 uL) with the yield of 178 mg and 82 mg, respectively. Furthermore, the structures of the obtained compounds were identified as cis- and trans-asarone by (1)H- and (13)C-NMR spectra, respectively.

No MeSH data available.


Related in: MedlinePlus

pGC-FID chromatogram for asarone isomers.
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Related In: Results  -  Collection


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fig1: pGC-FID chromatogram for asarone isomers.

Mentions: The GC chromatogram of asarone isomers mixture recorded by pGC with FID detection is given in Figure 1. It was used as a basis for the collection of two fractions (F1 and F2) that were analyzed by the capillary GC and GC-MS system for an evaluation of resolution and yields of the pGC. After 90 injections, it was resulting in amounts of 178 mg (F1) and 82 mg (F2) for the compounds in the respective traps, respectively.


Separation of cis- and trans-Asarone from Acorus tatarinowii by Preparative Gas Chromatography.

Zuo HL, Yang FQ, Zhang XM, Xia ZN - J Anal Methods Chem (2012)

pGC-FID chromatogram for asarone isomers.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303141&req=5

fig1: pGC-FID chromatogram for asarone isomers.
Mentions: The GC chromatogram of asarone isomers mixture recorded by pGC with FID detection is given in Figure 1. It was used as a basis for the collection of two fractions (F1 and F2) that were analyzed by the capillary GC and GC-MS system for an evaluation of resolution and yields of the pGC. After 90 injections, it was resulting in amounts of 178 mg (F1) and 82 mg (F2) for the compounds in the respective traps, respectively.

Bottom Line: And then the fraction that contains mixture of the isomers was further separated by pGC.The compounds were separated on a stainless steel column packed with 10% OV-101 (3 m × 6 mm, i.d.), and then the effluent was split into two gas flows.Two isomers were collected after 90 single injections (5 uL) with the yield of 178 mg and 82 mg, respectively.

View Article: PubMed Central - PubMed

Affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China.

ABSTRACT
A preparative gas chromatography (pGC) method was developed for the separation of isomers (cis- and trans-asarone) from essential oil of Acorus tatarinowii. The oil was primarily fractionated by silica gel chromatography using different ratios of petroleum ether and ethyl acetate as gradient elution solvents. And then the fraction that contains mixture of the isomers was further separated by pGC. The compounds were separated on a stainless steel column packed with 10% OV-101 (3 m × 6 mm, i.d.), and then the effluent was split into two gas flows. One percent of the effluent passed to the flame ionization detector (FID) for detection and the remaining 99% was directed to the fraction collector. Two isomers were collected after 90 single injections (5 uL) with the yield of 178 mg and 82 mg, respectively. Furthermore, the structures of the obtained compounds were identified as cis- and trans-asarone by (1)H- and (13)C-NMR spectra, respectively.

No MeSH data available.


Related in: MedlinePlus