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Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement.

Bron JL, Mulder HW, Vonk LA, Doulabi BZ, Oudhoff MJ, Smit TH - J Mater Sci Mater Med (2012)

Bottom Line: The aim of current study was to assess whether NP and surrounding annulus fibrosus (AF) cells are capable of migrating into dense collagen scaffolds.We seeded freshly harvested caprine NP and AF cells onto scaffolds consisting of 1.5 and 3.0% type I collagen matrices, prepared by plastic compression, to assess cell invasion.The migration distance appeared both time and density dependent and was higher for NP (25%) compared to AF (10%) cells after 4 weeks.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, VU University Medical Center, Amsterdam, The Netherlands. jl.bron@vumc.nl

ABSTRACT
Invasion of cells from surrounding tissues is a crucial step for regeneration when using a-cellular scaffolds as a replacement of the nucleus pulposus (NP). The aim of current study was to assess whether NP and surrounding annulus fibrosus (AF) cells are capable of migrating into dense collagen scaffolds. We seeded freshly harvested caprine NP and AF cells onto scaffolds consisting of 1.5 and 3.0% type I collagen matrices, prepared by plastic compression, to assess cell invasion. The migration distance appeared both time and density dependent and was higher for NP (25%) compared to AF (10%) cells after 4 weeks. Migration distance was not enhanced by Hst-2, a peptide derived from saliva known to enhance fibroblast migration, and this was confirmed in a scratch assay. In conclusion, we revealed invasion of cells into dense collagen scaffolds and therewith encouraging first steps towards the use of a-cellular scaffolds for NP replacement.

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Graphic showing the results of the migration experiments of NP in a 1.5% collagen matrix with and without the addition of Hst-2 as a chemo attractant. The results with the negative enantiomer DHst-2 are also shown. The samples with Hst2 and Dhst-2 reveal a significantly decreased migration after 28 days
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Fig6: Graphic showing the results of the migration experiments of NP in a 1.5% collagen matrix with and without the addition of Hst-2 as a chemo attractant. The results with the negative enantiomer DHst-2 are also shown. The samples with Hst2 and Dhst-2 reveal a significantly decreased migration after 28 days

Mentions: The addition of Hst-2 did not result in increased migration distance. Instead, in a large fraction of the AF cell samples, no cells could be detected after 14 or 28 days, or too few to perform analysis. Although some cell death was also observed in a few of the NP cell experiments, these data was sufficient for analysis. After 14 days, no differences were observed for NP cells with our without chemo attractant. After 28 days however, NP cells in the samples supplemented with Hst-2 (Fig. 6, grey bars) showed significantly less migration when compared to samples without chemo attractant (Fig. 6, black bars) The migration without chemoattractant was significantly higher compared to the migration with Hst2 or D-Hst2 (Fig. 6, white bars). Between both peptides, no significant differences were found.Fig. 6


Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement.

Bron JL, Mulder HW, Vonk LA, Doulabi BZ, Oudhoff MJ, Smit TH - J Mater Sci Mater Med (2012)

Graphic showing the results of the migration experiments of NP in a 1.5% collagen matrix with and without the addition of Hst-2 as a chemo attractant. The results with the negative enantiomer DHst-2 are also shown. The samples with Hst2 and Dhst-2 reveal a significantly decreased migration after 28 days
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3299969&req=5

Fig6: Graphic showing the results of the migration experiments of NP in a 1.5% collagen matrix with and without the addition of Hst-2 as a chemo attractant. The results with the negative enantiomer DHst-2 are also shown. The samples with Hst2 and Dhst-2 reveal a significantly decreased migration after 28 days
Mentions: The addition of Hst-2 did not result in increased migration distance. Instead, in a large fraction of the AF cell samples, no cells could be detected after 14 or 28 days, or too few to perform analysis. Although some cell death was also observed in a few of the NP cell experiments, these data was sufficient for analysis. After 14 days, no differences were observed for NP cells with our without chemo attractant. After 28 days however, NP cells in the samples supplemented with Hst-2 (Fig. 6, grey bars) showed significantly less migration when compared to samples without chemo attractant (Fig. 6, black bars) The migration without chemoattractant was significantly higher compared to the migration with Hst2 or D-Hst2 (Fig. 6, white bars). Between both peptides, no significant differences were found.Fig. 6

Bottom Line: The aim of current study was to assess whether NP and surrounding annulus fibrosus (AF) cells are capable of migrating into dense collagen scaffolds.We seeded freshly harvested caprine NP and AF cells onto scaffolds consisting of 1.5 and 3.0% type I collagen matrices, prepared by plastic compression, to assess cell invasion.The migration distance appeared both time and density dependent and was higher for NP (25%) compared to AF (10%) cells after 4 weeks.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, VU University Medical Center, Amsterdam, The Netherlands. jl.bron@vumc.nl

ABSTRACT
Invasion of cells from surrounding tissues is a crucial step for regeneration when using a-cellular scaffolds as a replacement of the nucleus pulposus (NP). The aim of current study was to assess whether NP and surrounding annulus fibrosus (AF) cells are capable of migrating into dense collagen scaffolds. We seeded freshly harvested caprine NP and AF cells onto scaffolds consisting of 1.5 and 3.0% type I collagen matrices, prepared by plastic compression, to assess cell invasion. The migration distance appeared both time and density dependent and was higher for NP (25%) compared to AF (10%) cells after 4 weeks. Migration distance was not enhanced by Hst-2, a peptide derived from saliva known to enhance fibroblast migration, and this was confirmed in a scratch assay. In conclusion, we revealed invasion of cells into dense collagen scaffolds and therewith encouraging first steps towards the use of a-cellular scaffolds for NP replacement.

Show MeSH
Related in: MedlinePlus