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Guava leaf extracts alleviate fatty liver via expression of adiponectin receptors in SHRSP.Z-Leprfa/Izm rats.

Yoshitomi H, Guo X, Liu T, Gao M - Nutr Metab (Lond) (2012)

Bottom Line: These were thought to be associated with the decrease of the triglyceride content in the liver because AMPK and PPARα in liver tissue control energy metabolism or lipid composition.Our results indicate that administration of GLE may have preventive effects of hepatic accumulation and ameliorated hepatic insulin resistance by enhancing the adiponectin beta-oxidation system.Guava leaf may be potentially useful for hepatic steatosis without the side effects of long-term treatments.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan. gaoming@mukogawa-u.ac.jp.

ABSTRACT

Background: In recent years, the number of people with metabolic syndrome has continued to rise because of changing eating habits, and accompanying hepatic steatosis patients have also increased. This study examined the effect of guava leaf extract on liver fat accumulation using SHRSP.Z-Leprfa/IzmDmcr rats (SHRSP/ZF), which are a metabolic syndrome model animal.

Method: Seven-week-old male SHRSP/ZF rats were divided into two groups, a control group and a guava leaf extract (GLE) group. We gave 2 g/kg/day GLE or water by forced administration for 6 weeks. After the experimental period, the rats were sacrificed and organ weight, hepatic lipids, serum aminotransferase and liver pathology were examined. To search for a possible mechanism, we examined the changes of key enzyme and transcriptional factors involved in hepatic fatty acid beta-oxidation.

Results: The triglyceride content of the liver significantly decreased in the GLE group in comparison with the control group, and decreased fat-drop formation in the liver tissue graft in the GLE group was observed. In addition, the improvement of liver organization impairments with fat accumulation restriction was suggested because blood AST and ALT in the GLE group significantly decreased. Furthermore, it was supposed that the activity of AMPK and PPARα significantly increased in the GLE group via the increase of adiponectin receptors. These were thought to be associated with the decrease of the triglyceride content in the liver because AMPK and PPARα in liver tissue control energy metabolism or lipid composition. On the other hand, insulin resistance was suggested to have improved by the fatty liver improvement in GLE.

Conclusion: Our results indicate that administration of GLE may have preventive effects of hepatic accumulation and ameliorated hepatic insulin resistance by enhancing the adiponectin beta-oxidation system. Guava leaf may be potentially useful for hepatic steatosis without the side effects of long-term treatments.

No MeSH data available.


Related in: MedlinePlus

Pathogenic description of the rat livers. Effect of oral administration of GLE on the histological examination of livers from SHRSP-fatty rats. Liver tissue sections were stained with HE and examined under a light microscope. Data are representative images (magnification × 100).
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Figure 1: Pathogenic description of the rat livers. Effect of oral administration of GLE on the histological examination of livers from SHRSP-fatty rats. Liver tissue sections were stained with HE and examined under a light microscope. Data are representative images (magnification × 100).

Mentions: The histological examination of livers from the experimental rats is shown in Figure 1. It can be seen that livers in the control group appeared to show a fatty liver. Administration of GLE showed a positive effect, and the GLE group improved and the liver appeared to be less fatty than the control group in the filtration of hepatocytes. This result was exemplified by the fact that levels of serum alanine transaminase (ALT) and aspartate transaminase (AST) in the GLE group were significantly lower than in the control group (Table 3: p = 0.019 and p = 0.049).


Guava leaf extracts alleviate fatty liver via expression of adiponectin receptors in SHRSP.Z-Leprfa/Izm rats.

Yoshitomi H, Guo X, Liu T, Gao M - Nutr Metab (Lond) (2012)

Pathogenic description of the rat livers. Effect of oral administration of GLE on the histological examination of livers from SHRSP-fatty rats. Liver tissue sections were stained with HE and examined under a light microscope. Data are representative images (magnification × 100).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3298795&req=5

Figure 1: Pathogenic description of the rat livers. Effect of oral administration of GLE on the histological examination of livers from SHRSP-fatty rats. Liver tissue sections were stained with HE and examined under a light microscope. Data are representative images (magnification × 100).
Mentions: The histological examination of livers from the experimental rats is shown in Figure 1. It can be seen that livers in the control group appeared to show a fatty liver. Administration of GLE showed a positive effect, and the GLE group improved and the liver appeared to be less fatty than the control group in the filtration of hepatocytes. This result was exemplified by the fact that levels of serum alanine transaminase (ALT) and aspartate transaminase (AST) in the GLE group were significantly lower than in the control group (Table 3: p = 0.019 and p = 0.049).

Bottom Line: These were thought to be associated with the decrease of the triglyceride content in the liver because AMPK and PPARα in liver tissue control energy metabolism or lipid composition.Our results indicate that administration of GLE may have preventive effects of hepatic accumulation and ameliorated hepatic insulin resistance by enhancing the adiponectin beta-oxidation system.Guava leaf may be potentially useful for hepatic steatosis without the side effects of long-term treatments.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan. gaoming@mukogawa-u.ac.jp.

ABSTRACT

Background: In recent years, the number of people with metabolic syndrome has continued to rise because of changing eating habits, and accompanying hepatic steatosis patients have also increased. This study examined the effect of guava leaf extract on liver fat accumulation using SHRSP.Z-Leprfa/IzmDmcr rats (SHRSP/ZF), which are a metabolic syndrome model animal.

Method: Seven-week-old male SHRSP/ZF rats were divided into two groups, a control group and a guava leaf extract (GLE) group. We gave 2 g/kg/day GLE or water by forced administration for 6 weeks. After the experimental period, the rats were sacrificed and organ weight, hepatic lipids, serum aminotransferase and liver pathology were examined. To search for a possible mechanism, we examined the changes of key enzyme and transcriptional factors involved in hepatic fatty acid beta-oxidation.

Results: The triglyceride content of the liver significantly decreased in the GLE group in comparison with the control group, and decreased fat-drop formation in the liver tissue graft in the GLE group was observed. In addition, the improvement of liver organization impairments with fat accumulation restriction was suggested because blood AST and ALT in the GLE group significantly decreased. Furthermore, it was supposed that the activity of AMPK and PPARα significantly increased in the GLE group via the increase of adiponectin receptors. These were thought to be associated with the decrease of the triglyceride content in the liver because AMPK and PPARα in liver tissue control energy metabolism or lipid composition. On the other hand, insulin resistance was suggested to have improved by the fatty liver improvement in GLE.

Conclusion: Our results indicate that administration of GLE may have preventive effects of hepatic accumulation and ameliorated hepatic insulin resistance by enhancing the adiponectin beta-oxidation system. Guava leaf may be potentially useful for hepatic steatosis without the side effects of long-term treatments.

No MeSH data available.


Related in: MedlinePlus