No impairment of monocyte-derived Langerhans cell phenotype or function in early-onset psoriasis.
Bottom Line: The cytokine and chemokine content of supernatants was analysed by cytokine array.CD14+ cells acquired an LC-like phenotype with high expression of CD1a and major histocompatibility complex (MHC) class II.Moreover, mLCs isolated from both groups displayed comparable ability to migrate in vitro.
Affiliation: Toxicology Group, Faculty of Life Sciences, University of Manchester, Manchester, UK. email@example.comShow MeSH
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Mentions: Following culture with GM-CSF, IL-4 and TGF-β, CD14+ monocytes derived from both healthy controls and patients with psoriasis acquired a DC-like phenotype, with CD14 expression decreasing to negligible levels in both groups. Concurrently, the percentage of CD1a+ cells at day 6 were 89.3 ± 3.8 and 88.9 ± 2.6%, in controls and patients, respectively, demonstrating that there was no impairment in the ability of monocytes obtained from patients with early-onset psoriasis to differentiate into DC-like cells (Fig. 1).
Affiliation: Toxicology Group, Faculty of Life Sciences, University of Manchester, Manchester, UK. firstname.lastname@example.org