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No impairment of monocyte-derived Langerhans cell phenotype or function in early-onset psoriasis.

Shaw FL, Kimber I, Begum R, Cumberbatch M, Dearman RJ, Griffiths CE - Clin. Exp. Dermatol. (2011)

Bottom Line: The cytokine and chemokine content of supernatants was analysed by cytokine array.CD14+ cells acquired an LC-like phenotype with high expression of CD1a and major histocompatibility complex (MHC) class II.Moreover, mLCs isolated from both groups displayed comparable ability to migrate in vitro.

View Article: PubMed Central - PubMed

Affiliation: Toxicology Group, Faculty of Life Sciences, University of Manchester, Manchester, UK. frances.shaw@manchester.ac.uk

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Related in: MedlinePlus

Representative histograms demonstrating mean fluorescence intensity of CD1a, CD86, CD54 and major histocompatibility complex class II by monocyte-derived Langerhans cells derived from both (a) healthy controls and (b) patients with early-onset psoriasis. Each histogram displays data for unstimulated (solid line) and cytokine-stimulated (dashed line, tumour necrosis factor-α; dotted line, interleukin-1β; both 100 ng/mL) cells, in addition to an isotype control (shaded histogram). MHC, major histocompatibility complex.
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fig01: Representative histograms demonstrating mean fluorescence intensity of CD1a, CD86, CD54 and major histocompatibility complex class II by monocyte-derived Langerhans cells derived from both (a) healthy controls and (b) patients with early-onset psoriasis. Each histogram displays data for unstimulated (solid line) and cytokine-stimulated (dashed line, tumour necrosis factor-α; dotted line, interleukin-1β; both 100 ng/mL) cells, in addition to an isotype control (shaded histogram). MHC, major histocompatibility complex.

Mentions: Following culture with GM-CSF, IL-4 and TGF-β, CD14+ monocytes derived from both healthy controls and patients with psoriasis acquired a DC-like phenotype, with CD14 expression decreasing to negligible levels in both groups. Concurrently, the percentage of CD1a+ cells at day 6 were 89.3 ± 3.8 and 88.9 ± 2.6%, in controls and patients, respectively, demonstrating that there was no impairment in the ability of monocytes obtained from patients with early-onset psoriasis to differentiate into DC-like cells (Fig. 1).


No impairment of monocyte-derived Langerhans cell phenotype or function in early-onset psoriasis.

Shaw FL, Kimber I, Begum R, Cumberbatch M, Dearman RJ, Griffiths CE - Clin. Exp. Dermatol. (2011)

Representative histograms demonstrating mean fluorescence intensity of CD1a, CD86, CD54 and major histocompatibility complex class II by monocyte-derived Langerhans cells derived from both (a) healthy controls and (b) patients with early-onset psoriasis. Each histogram displays data for unstimulated (solid line) and cytokine-stimulated (dashed line, tumour necrosis factor-α; dotted line, interleukin-1β; both 100 ng/mL) cells, in addition to an isotype control (shaded histogram). MHC, major histocompatibility complex.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3298657&req=5

fig01: Representative histograms demonstrating mean fluorescence intensity of CD1a, CD86, CD54 and major histocompatibility complex class II by monocyte-derived Langerhans cells derived from both (a) healthy controls and (b) patients with early-onset psoriasis. Each histogram displays data for unstimulated (solid line) and cytokine-stimulated (dashed line, tumour necrosis factor-α; dotted line, interleukin-1β; both 100 ng/mL) cells, in addition to an isotype control (shaded histogram). MHC, major histocompatibility complex.
Mentions: Following culture with GM-CSF, IL-4 and TGF-β, CD14+ monocytes derived from both healthy controls and patients with psoriasis acquired a DC-like phenotype, with CD14 expression decreasing to negligible levels in both groups. Concurrently, the percentage of CD1a+ cells at day 6 were 89.3 ± 3.8 and 88.9 ± 2.6%, in controls and patients, respectively, demonstrating that there was no impairment in the ability of monocytes obtained from patients with early-onset psoriasis to differentiate into DC-like cells (Fig. 1).

Bottom Line: The cytokine and chemokine content of supernatants was analysed by cytokine array.CD14+ cells acquired an LC-like phenotype with high expression of CD1a and major histocompatibility complex (MHC) class II.Moreover, mLCs isolated from both groups displayed comparable ability to migrate in vitro.

View Article: PubMed Central - PubMed

Affiliation: Toxicology Group, Faculty of Life Sciences, University of Manchester, Manchester, UK. frances.shaw@manchester.ac.uk

Show MeSH
Related in: MedlinePlus