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MQD--multiplex-quadrature detection in multi-dimensional NMR.

Schlagnitweit J, Horničáková M, Zuckerstätter G, Müller N - Chemphyschem (2011)

Bottom Line: Thus the number of acquisitions required to achieve a desired resolution in the indirect dimensions is significantly reduced.The minimum number of transients per indirect data point, which have to be combined to give pure-phase spectra, is thus decreased by a factor (3/4)(N-1).This reduction is achieved without adjustable parameters.

View Article: PubMed Central - PubMed

Affiliation: Institute of Organic Chemistry, Johannes Kepler University Linz, Altenberger Str. 69, 4040 Linz, Austria.

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2D HNCO spectra of ubiquitin with a) MQD, b) States, without axial peak suppression phase cycle, c) States TPPI without axial peak suppression phase cycle and d) States TPPI with axial peak suppression (0°, 180°) phase cycle. The pulse lengths were misset to 90 % of the calibrated values. It can be seen that even under such unfavorable conditions the axial peak and solvent-artifact suppression efficiency of the MQD experiment (a) is equivalent to the one in the standard phase-cycled experiment (d). In all these plots, the contour levels are equal.
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fig03: 2D HNCO spectra of ubiquitin with a) MQD, b) States, without axial peak suppression phase cycle, c) States TPPI without axial peak suppression phase cycle and d) States TPPI with axial peak suppression (0°, 180°) phase cycle. The pulse lengths were misset to 90 % of the calibrated values. It can be seen that even under such unfavorable conditions the axial peak and solvent-artifact suppression efficiency of the MQD experiment (a) is equivalent to the one in the standard phase-cycled experiment (d). In all these plots, the contour levels are equal.

Mentions: To assess the selection efficiency of the multiplex-phase cycled experiments the programs CCCP23 (for simulating coherence-transfer pathways) and POMA24 (for Cartesian product operator calculations25) were used. The results of the MQD pulse sequences for basic 2D and 3D NMR experiments are summarized in Figure 1. The spectra shown in Figure 3 comparing MQD to States and States-TPPI applying deliberately misset pulses, with and without phase cycling for axial peak suppression, prove the axial peak suppression efficiency to be on par with the slower classical approach.


MQD--multiplex-quadrature detection in multi-dimensional NMR.

Schlagnitweit J, Horničáková M, Zuckerstätter G, Müller N - Chemphyschem (2011)

2D HNCO spectra of ubiquitin with a) MQD, b) States, without axial peak suppression phase cycle, c) States TPPI without axial peak suppression phase cycle and d) States TPPI with axial peak suppression (0°, 180°) phase cycle. The pulse lengths were misset to 90 % of the calibrated values. It can be seen that even under such unfavorable conditions the axial peak and solvent-artifact suppression efficiency of the MQD experiment (a) is equivalent to the one in the standard phase-cycled experiment (d). In all these plots, the contour levels are equal.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3298640&req=5

fig03: 2D HNCO spectra of ubiquitin with a) MQD, b) States, without axial peak suppression phase cycle, c) States TPPI without axial peak suppression phase cycle and d) States TPPI with axial peak suppression (0°, 180°) phase cycle. The pulse lengths were misset to 90 % of the calibrated values. It can be seen that even under such unfavorable conditions the axial peak and solvent-artifact suppression efficiency of the MQD experiment (a) is equivalent to the one in the standard phase-cycled experiment (d). In all these plots, the contour levels are equal.
Mentions: To assess the selection efficiency of the multiplex-phase cycled experiments the programs CCCP23 (for simulating coherence-transfer pathways) and POMA24 (for Cartesian product operator calculations25) were used. The results of the MQD pulse sequences for basic 2D and 3D NMR experiments are summarized in Figure 1. The spectra shown in Figure 3 comparing MQD to States and States-TPPI applying deliberately misset pulses, with and without phase cycling for axial peak suppression, prove the axial peak suppression efficiency to be on par with the slower classical approach.

Bottom Line: Thus the number of acquisitions required to achieve a desired resolution in the indirect dimensions is significantly reduced.The minimum number of transients per indirect data point, which have to be combined to give pure-phase spectra, is thus decreased by a factor (3/4)(N-1).This reduction is achieved without adjustable parameters.

View Article: PubMed Central - PubMed

Affiliation: Institute of Organic Chemistry, Johannes Kepler University Linz, Altenberger Str. 69, 4040 Linz, Austria.

Show MeSH
Related in: MedlinePlus