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KLF6SV1 siRNA inhibits proliferation of human lens epithelial cells.

Su Y, Qu Y, Jiang C, Liu L, Shan Y, Wang F - Mol. Vis. (2012)

Bottom Line: KLF6SV1 siRNA can decrease KLF6SV1 expression which leads to increased levels of p27(kip1) and decreased expression of PCNA in HLEC.Cells transfected with pKLF6SV1 siRNA showed less viability compared with the control group in vitro.KLF6SV1 siRNA can effectively inhibit HLEC proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, First clinical college of Harbin Medical University, Harbin, China.

ABSTRACT

Purpose: To investigate whether transfection with Krüppel-like factor 6 splice variant 1 (KLF6SV1) siRNA can inhibit proliferation of human lens epithelial cell (HLEC).

Methods: Plasmid containing KLF6SV1 siRNA was used to decrease the level of KLF6SV1 protein in HLEC. The expression of protein27 kinase inhibition protein 1 (p27(kip1)) and proliferation cell nuclear antigen (PCNA) was tested with western blot. Cell proliferation was assayed by 3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazoliumbromide (MTT) assay and bromodeoxyuridine (BrdU) incorporation.

Results: KLF6SV1 siRNA can decrease KLF6SV1 expression which leads to increased levels of p27(kip1) and decreased expression of PCNA in HLEC. Cells transfected with pKLF6SV1 siRNA showed less viability compared with the control group in vitro.

Conclusions: KLF6SV1 siRNA can effectively inhibit HLEC proliferation. It can be regarded as a novel target to treat posterior capsular opacity (PCO).

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Related in: MedlinePlus

Cell viability assay by MTT. Cell viability in HLEC significantly decreased by the 5th day after transfection with pKLF6SV1 siRNA compared with vehicle control and blank control.
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f5: Cell viability assay by MTT. Cell viability in HLEC significantly decreased by the 5th day after transfection with pKLF6SV1 siRNA compared with vehicle control and blank control.

Mentions: Cell viability in pKLF6SV1 siRNA transfected HLEC significantly decreased by the 5th day after transfection when compared with vehicle and blank control (Figure 5).


KLF6SV1 siRNA inhibits proliferation of human lens epithelial cells.

Su Y, Qu Y, Jiang C, Liu L, Shan Y, Wang F - Mol. Vis. (2012)

Cell viability assay by MTT. Cell viability in HLEC significantly decreased by the 5th day after transfection with pKLF6SV1 siRNA compared with vehicle control and blank control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3298452&req=5

f5: Cell viability assay by MTT. Cell viability in HLEC significantly decreased by the 5th day after transfection with pKLF6SV1 siRNA compared with vehicle control and blank control.
Mentions: Cell viability in pKLF6SV1 siRNA transfected HLEC significantly decreased by the 5th day after transfection when compared with vehicle and blank control (Figure 5).

Bottom Line: KLF6SV1 siRNA can decrease KLF6SV1 expression which leads to increased levels of p27(kip1) and decreased expression of PCNA in HLEC.Cells transfected with pKLF6SV1 siRNA showed less viability compared with the control group in vitro.KLF6SV1 siRNA can effectively inhibit HLEC proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, First clinical college of Harbin Medical University, Harbin, China.

ABSTRACT

Purpose: To investigate whether transfection with Krüppel-like factor 6 splice variant 1 (KLF6SV1) siRNA can inhibit proliferation of human lens epithelial cell (HLEC).

Methods: Plasmid containing KLF6SV1 siRNA was used to decrease the level of KLF6SV1 protein in HLEC. The expression of protein27 kinase inhibition protein 1 (p27(kip1)) and proliferation cell nuclear antigen (PCNA) was tested with western blot. Cell proliferation was assayed by 3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazoliumbromide (MTT) assay and bromodeoxyuridine (BrdU) incorporation.

Results: KLF6SV1 siRNA can decrease KLF6SV1 expression which leads to increased levels of p27(kip1) and decreased expression of PCNA in HLEC. Cells transfected with pKLF6SV1 siRNA showed less viability compared with the control group in vitro.

Conclusions: KLF6SV1 siRNA can effectively inhibit HLEC proliferation. It can be regarded as a novel target to treat posterior capsular opacity (PCO).

Show MeSH
Related in: MedlinePlus