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KLF6SV1 siRNA inhibits proliferation of human lens epithelial cells.

Su Y, Qu Y, Jiang C, Liu L, Shan Y, Wang F - Mol. Vis. (2012)

Bottom Line: KLF6SV1 siRNA can decrease KLF6SV1 expression which leads to increased levels of p27(kip1) and decreased expression of PCNA in HLEC.Cells transfected with pKLF6SV1 siRNA showed less viability compared with the control group in vitro.KLF6SV1 siRNA can effectively inhibit HLEC proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, First clinical college of Harbin Medical University, Harbin, China.

ABSTRACT

Purpose: To investigate whether transfection with Krüppel-like factor 6 splice variant 1 (KLF6SV1) siRNA can inhibit proliferation of human lens epithelial cell (HLEC).

Methods: Plasmid containing KLF6SV1 siRNA was used to decrease the level of KLF6SV1 protein in HLEC. The expression of protein27 kinase inhibition protein 1 (p27(kip1)) and proliferation cell nuclear antigen (PCNA) was tested with western blot. Cell proliferation was assayed by 3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazoliumbromide (MTT) assay and bromodeoxyuridine (BrdU) incorporation.

Results: KLF6SV1 siRNA can decrease KLF6SV1 expression which leads to increased levels of p27(kip1) and decreased expression of PCNA in HLEC. Cells transfected with pKLF6SV1 siRNA showed less viability compared with the control group in vitro.

Conclusions: KLF6SV1 siRNA can effectively inhibit HLEC proliferation. It can be regarded as a novel target to treat posterior capsular opacity (PCO).

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Related in: MedlinePlus

Upregulation of p27kip1 in HLEC transfected with pKLF6SV1 siRNA. Expression of p27kip1 increased in HLEC transfected with pKLFSV1 siRNA (lane 3) compared with vehicle control (lane 1) and blank control (lane 2).
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f2: Upregulation of p27kip1 in HLEC transfected with pKLF6SV1 siRNA. Expression of p27kip1 increased in HLEC transfected with pKLFSV1 siRNA (lane 3) compared with vehicle control (lane 1) and blank control (lane 2).

Mentions: Upregualtion of p27kip1 by transfection of skp2 siRNA can inhibit the proliferation of rabbit tennon’s fibroblast cells after glaucoma surgery which indicate it is important for inhibition of cell proliferation [18]. Expression of p27kip1 increased in HLEC transfected with pKLFSV1 siRNA (Figure 2, lane 3) compared with vehicle control (Figure 2, lane 1) and blank control (Figure 2, lane 2). Upregulation of p27 kip1 made it possible for us to investigate the effect of p27 kip1 inhibition on the proliferation of HLEC.


KLF6SV1 siRNA inhibits proliferation of human lens epithelial cells.

Su Y, Qu Y, Jiang C, Liu L, Shan Y, Wang F - Mol. Vis. (2012)

Upregulation of p27kip1 in HLEC transfected with pKLF6SV1 siRNA. Expression of p27kip1 increased in HLEC transfected with pKLFSV1 siRNA (lane 3) compared with vehicle control (lane 1) and blank control (lane 2).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3298452&req=5

f2: Upregulation of p27kip1 in HLEC transfected with pKLF6SV1 siRNA. Expression of p27kip1 increased in HLEC transfected with pKLFSV1 siRNA (lane 3) compared with vehicle control (lane 1) and blank control (lane 2).
Mentions: Upregualtion of p27kip1 by transfection of skp2 siRNA can inhibit the proliferation of rabbit tennon’s fibroblast cells after glaucoma surgery which indicate it is important for inhibition of cell proliferation [18]. Expression of p27kip1 increased in HLEC transfected with pKLFSV1 siRNA (Figure 2, lane 3) compared with vehicle control (Figure 2, lane 1) and blank control (Figure 2, lane 2). Upregulation of p27 kip1 made it possible for us to investigate the effect of p27 kip1 inhibition on the proliferation of HLEC.

Bottom Line: KLF6SV1 siRNA can decrease KLF6SV1 expression which leads to increased levels of p27(kip1) and decreased expression of PCNA in HLEC.Cells transfected with pKLF6SV1 siRNA showed less viability compared with the control group in vitro.KLF6SV1 siRNA can effectively inhibit HLEC proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, First clinical college of Harbin Medical University, Harbin, China.

ABSTRACT

Purpose: To investigate whether transfection with Krüppel-like factor 6 splice variant 1 (KLF6SV1) siRNA can inhibit proliferation of human lens epithelial cell (HLEC).

Methods: Plasmid containing KLF6SV1 siRNA was used to decrease the level of KLF6SV1 protein in HLEC. The expression of protein27 kinase inhibition protein 1 (p27(kip1)) and proliferation cell nuclear antigen (PCNA) was tested with western blot. Cell proliferation was assayed by 3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazoliumbromide (MTT) assay and bromodeoxyuridine (BrdU) incorporation.

Results: KLF6SV1 siRNA can decrease KLF6SV1 expression which leads to increased levels of p27(kip1) and decreased expression of PCNA in HLEC. Cells transfected with pKLF6SV1 siRNA showed less viability compared with the control group in vitro.

Conclusions: KLF6SV1 siRNA can effectively inhibit HLEC proliferation. It can be regarded as a novel target to treat posterior capsular opacity (PCO).

Show MeSH
Related in: MedlinePlus