Limits...
KLF6SV1 siRNA inhibits proliferation of human lens epithelial cells.

Su Y, Qu Y, Jiang C, Liu L, Shan Y, Wang F - Mol. Vis. (2012)

Bottom Line: KLF6SV1 siRNA can decrease KLF6SV1 expression which leads to increased levels of p27(kip1) and decreased expression of PCNA in HLEC.Cells transfected with pKLF6SV1 siRNA showed less viability compared with the control group in vitro.KLF6SV1 siRNA can effectively inhibit HLEC proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, First clinical college of Harbin Medical University, Harbin, China.

ABSTRACT

Purpose: To investigate whether transfection with Krüppel-like factor 6 splice variant 1 (KLF6SV1) siRNA can inhibit proliferation of human lens epithelial cell (HLEC).

Methods: Plasmid containing KLF6SV1 siRNA was used to decrease the level of KLF6SV1 protein in HLEC. The expression of protein27 kinase inhibition protein 1 (p27(kip1)) and proliferation cell nuclear antigen (PCNA) was tested with western blot. Cell proliferation was assayed by 3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazoliumbromide (MTT) assay and bromodeoxyuridine (BrdU) incorporation.

Results: KLF6SV1 siRNA can decrease KLF6SV1 expression which leads to increased levels of p27(kip1) and decreased expression of PCNA in HLEC. Cells transfected with pKLF6SV1 siRNA showed less viability compared with the control group in vitro.

Conclusions: KLF6SV1 siRNA can effectively inhibit HLEC proliferation. It can be regarded as a novel target to treat posterior capsular opacity (PCO).

Show MeSH

Related in: MedlinePlus

Downregulation of KLF6SV1 in HLEC transfected with pKLF6SV1 siRNA. There is KLF6SV1 expression in the HLEC transfected with vehicle control (lane 1) or blank control (lane 2). Little expression of KLF6SV1 was detected in HLEC transfected with pKLFSV1 siRNA which indicates KLF6SV1siRNA can effectively decrease the expression of KLF6SV1 (lane 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3298452&req=5

f1: Downregulation of KLF6SV1 in HLEC transfected with pKLF6SV1 siRNA. There is KLF6SV1 expression in the HLEC transfected with vehicle control (lane 1) or blank control (lane 2). Little expression of KLF6SV1 was detected in HLEC transfected with pKLFSV1 siRNA which indicates KLF6SV1siRNA can effectively decrease the expression of KLF6SV1 (lane 3).

Mentions: We explored the biologic role of KLF6-SV1 using siRNA-mediated gene silencing to specifically downregulate its expression. There is expression of KLF6SV1 protein in the HLEC transfected with vehicle (Figure 1, lane 3) or without transfection (Figure 1, lane 2). Little expression of KLF6SV1 was detected in HLEC transfected with pKLFSV1 siRNA (Figure 1, lane 3).


KLF6SV1 siRNA inhibits proliferation of human lens epithelial cells.

Su Y, Qu Y, Jiang C, Liu L, Shan Y, Wang F - Mol. Vis. (2012)

Downregulation of KLF6SV1 in HLEC transfected with pKLF6SV1 siRNA. There is KLF6SV1 expression in the HLEC transfected with vehicle control (lane 1) or blank control (lane 2). Little expression of KLF6SV1 was detected in HLEC transfected with pKLFSV1 siRNA which indicates KLF6SV1siRNA can effectively decrease the expression of KLF6SV1 (lane 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3298452&req=5

f1: Downregulation of KLF6SV1 in HLEC transfected with pKLF6SV1 siRNA. There is KLF6SV1 expression in the HLEC transfected with vehicle control (lane 1) or blank control (lane 2). Little expression of KLF6SV1 was detected in HLEC transfected with pKLFSV1 siRNA which indicates KLF6SV1siRNA can effectively decrease the expression of KLF6SV1 (lane 3).
Mentions: We explored the biologic role of KLF6-SV1 using siRNA-mediated gene silencing to specifically downregulate its expression. There is expression of KLF6SV1 protein in the HLEC transfected with vehicle (Figure 1, lane 3) or without transfection (Figure 1, lane 2). Little expression of KLF6SV1 was detected in HLEC transfected with pKLFSV1 siRNA (Figure 1, lane 3).

Bottom Line: KLF6SV1 siRNA can decrease KLF6SV1 expression which leads to increased levels of p27(kip1) and decreased expression of PCNA in HLEC.Cells transfected with pKLF6SV1 siRNA showed less viability compared with the control group in vitro.KLF6SV1 siRNA can effectively inhibit HLEC proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, First clinical college of Harbin Medical University, Harbin, China.

ABSTRACT

Purpose: To investigate whether transfection with Krüppel-like factor 6 splice variant 1 (KLF6SV1) siRNA can inhibit proliferation of human lens epithelial cell (HLEC).

Methods: Plasmid containing KLF6SV1 siRNA was used to decrease the level of KLF6SV1 protein in HLEC. The expression of protein27 kinase inhibition protein 1 (p27(kip1)) and proliferation cell nuclear antigen (PCNA) was tested with western blot. Cell proliferation was assayed by 3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazoliumbromide (MTT) assay and bromodeoxyuridine (BrdU) incorporation.

Results: KLF6SV1 siRNA can decrease KLF6SV1 expression which leads to increased levels of p27(kip1) and decreased expression of PCNA in HLEC. Cells transfected with pKLF6SV1 siRNA showed less viability compared with the control group in vitro.

Conclusions: KLF6SV1 siRNA can effectively inhibit HLEC proliferation. It can be regarded as a novel target to treat posterior capsular opacity (PCO).

Show MeSH
Related in: MedlinePlus