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Evaluation of uptake and transport of cationic and anionic ultrasmall iron oxide nanoparticles by human colon cells.

Kenzaoui BH, Vilà MR, Miquel JM, Cengelli F, Juillerat-Jeanneret L - Int J Nanomedicine (2012)

Bottom Line: The intracellular localization of aminoPVA USPIO NPs was confirmed in HT-29 cells by transmission electron microscopy that detected the iron oxide core.AminoPVA USPIO NPs invaded three-dimensional spheroids of both HT-29 and Caco-2 cells, whereas oleic acid-coated USPIO NPs could only invade Caco-2 spheroids.Neither cationic aminoPVA USPIO NPs nor anionic oleic acid-coated USPIO NPs were transported at detectable levels across the tight CacoReady™ intestinal barrier model or the more permeable mucus-secreting CacoGoblet™ model.

View Article: PubMed Central - PubMed

Affiliation: Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.

ABSTRACT
Nanoparticles (NPs) are in clinical use or under development for therapeutic imaging and drug delivery. However, relatively little information exists concerning the uptake and transport of NPs across human colon cell layers, or their potential to invade three-dimensional models of human colon cells that better mimic the tissue structures of normal and tumoral colon. In order to gain such information, the interactions of biocompatible ultrasmall superparamagnetic iron oxide nanoparticles (USPIO NPs) (iron oxide core 9-10 nm) coated with either cationic polyvinylamine (aminoPVA) or anionic oleic acid with human HT-29 and Caco-2 colon cells was determined. The uptake of the cationic USPIO NPs was much higher than the uptake of the anionic USPIO NPs. The intracellular localization of aminoPVA USPIO NPs was confirmed in HT-29 cells by transmission electron microscopy that detected the iron oxide core. AminoPVA USPIO NPs invaded three-dimensional spheroids of both HT-29 and Caco-2 cells, whereas oleic acid-coated USPIO NPs could only invade Caco-2 spheroids. Neither cationic aminoPVA USPIO NPs nor anionic oleic acid-coated USPIO NPs were transported at detectable levels across the tight CacoReady™ intestinal barrier model or the more permeable mucus-secreting CacoGoblet™ model.

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Transport of ultrasmall superparamagnetic iron oxide nanoparticles (USPIO NPs) by human colon cells. Transport of polyvinylamine (aminoPVA) USPIO NPs (A) and oleic acid-coated USPIO NPs (B) across CacoReady™ (white bars) and CacoGobletTM (grey bars) colon barriers and across empty membrane (black bars). The initial concentration of iron in apical chamber was 100 μg/mL (A) or 70 μg/mL (B). Iron concentration in the basal chamber was quantified with the Prussian blue reaction.Note: The results represent noncumulated amounts of iron in the basal compartments at each time-point, without addition of USPIO NPs in the apical compartment.
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f6-ijn-7-1275: Transport of ultrasmall superparamagnetic iron oxide nanoparticles (USPIO NPs) by human colon cells. Transport of polyvinylamine (aminoPVA) USPIO NPs (A) and oleic acid-coated USPIO NPs (B) across CacoReady™ (white bars) and CacoGobletTM (grey bars) colon barriers and across empty membrane (black bars). The initial concentration of iron in apical chamber was 100 μg/mL (A) or 70 μg/mL (B). Iron concentration in the basal chamber was quantified with the Prussian blue reaction.Note: The results represent noncumulated amounts of iron in the basal compartments at each time-point, without addition of USPIO NPs in the apical compartment.

Mentions: The ability of two different cellular experimental models of the gastrointestinal barrier (the tight CacoReady model and the more permeable mucus-secreting CacoGoblet model) to transport cationic and anionic USPIO NPs was evaluated. Cells were exposed to the USPIO NPs for 24 hours after which LY permeability was assessed, and this demonstrated that the presence of USPIO NPs did not affect the integrity of the CacoReady and CacoGoblet barriers (results not shown). The transport experiments performed for a 24-hour time-course showed that whereas the USPIO NPs were transported across empty Transwell membranes, neither gastrointestinal barrier models efficiently transported the USPIO NPs (Figure 6). The noncumulated amount of USPIO NPs in the basal compartment of the device showed that oleic acid-coated USPIO NPs transfer less across the membrane than the aminoPVA USPIO NPs, with most of the transfer of oleic acid-coated USPIO NPs being achieved within 2 hours.


Evaluation of uptake and transport of cationic and anionic ultrasmall iron oxide nanoparticles by human colon cells.

Kenzaoui BH, Vilà MR, Miquel JM, Cengelli F, Juillerat-Jeanneret L - Int J Nanomedicine (2012)

Transport of ultrasmall superparamagnetic iron oxide nanoparticles (USPIO NPs) by human colon cells. Transport of polyvinylamine (aminoPVA) USPIO NPs (A) and oleic acid-coated USPIO NPs (B) across CacoReady™ (white bars) and CacoGobletTM (grey bars) colon barriers and across empty membrane (black bars). The initial concentration of iron in apical chamber was 100 μg/mL (A) or 70 μg/mL (B). Iron concentration in the basal chamber was quantified with the Prussian blue reaction.Note: The results represent noncumulated amounts of iron in the basal compartments at each time-point, without addition of USPIO NPs in the apical compartment.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3298390&req=5

f6-ijn-7-1275: Transport of ultrasmall superparamagnetic iron oxide nanoparticles (USPIO NPs) by human colon cells. Transport of polyvinylamine (aminoPVA) USPIO NPs (A) and oleic acid-coated USPIO NPs (B) across CacoReady™ (white bars) and CacoGobletTM (grey bars) colon barriers and across empty membrane (black bars). The initial concentration of iron in apical chamber was 100 μg/mL (A) or 70 μg/mL (B). Iron concentration in the basal chamber was quantified with the Prussian blue reaction.Note: The results represent noncumulated amounts of iron in the basal compartments at each time-point, without addition of USPIO NPs in the apical compartment.
Mentions: The ability of two different cellular experimental models of the gastrointestinal barrier (the tight CacoReady model and the more permeable mucus-secreting CacoGoblet model) to transport cationic and anionic USPIO NPs was evaluated. Cells were exposed to the USPIO NPs for 24 hours after which LY permeability was assessed, and this demonstrated that the presence of USPIO NPs did not affect the integrity of the CacoReady and CacoGoblet barriers (results not shown). The transport experiments performed for a 24-hour time-course showed that whereas the USPIO NPs were transported across empty Transwell membranes, neither gastrointestinal barrier models efficiently transported the USPIO NPs (Figure 6). The noncumulated amount of USPIO NPs in the basal compartment of the device showed that oleic acid-coated USPIO NPs transfer less across the membrane than the aminoPVA USPIO NPs, with most of the transfer of oleic acid-coated USPIO NPs being achieved within 2 hours.

Bottom Line: The intracellular localization of aminoPVA USPIO NPs was confirmed in HT-29 cells by transmission electron microscopy that detected the iron oxide core.AminoPVA USPIO NPs invaded three-dimensional spheroids of both HT-29 and Caco-2 cells, whereas oleic acid-coated USPIO NPs could only invade Caco-2 spheroids.Neither cationic aminoPVA USPIO NPs nor anionic oleic acid-coated USPIO NPs were transported at detectable levels across the tight CacoReady™ intestinal barrier model or the more permeable mucus-secreting CacoGoblet™ model.

View Article: PubMed Central - PubMed

Affiliation: Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.

ABSTRACT
Nanoparticles (NPs) are in clinical use or under development for therapeutic imaging and drug delivery. However, relatively little information exists concerning the uptake and transport of NPs across human colon cell layers, or their potential to invade three-dimensional models of human colon cells that better mimic the tissue structures of normal and tumoral colon. In order to gain such information, the interactions of biocompatible ultrasmall superparamagnetic iron oxide nanoparticles (USPIO NPs) (iron oxide core 9-10 nm) coated with either cationic polyvinylamine (aminoPVA) or anionic oleic acid with human HT-29 and Caco-2 colon cells was determined. The uptake of the cationic USPIO NPs was much higher than the uptake of the anionic USPIO NPs. The intracellular localization of aminoPVA USPIO NPs was confirmed in HT-29 cells by transmission electron microscopy that detected the iron oxide core. AminoPVA USPIO NPs invaded three-dimensional spheroids of both HT-29 and Caco-2 cells, whereas oleic acid-coated USPIO NPs could only invade Caco-2 spheroids. Neither cationic aminoPVA USPIO NPs nor anionic oleic acid-coated USPIO NPs were transported at detectable levels across the tight CacoReady™ intestinal barrier model or the more permeable mucus-secreting CacoGoblet™ model.

Show MeSH