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Evaluation of uptake and transport of cationic and anionic ultrasmall iron oxide nanoparticles by human colon cells.

Kenzaoui BH, Vilà MR, Miquel JM, Cengelli F, Juillerat-Jeanneret L - Int J Nanomedicine (2012)

Bottom Line: The intracellular localization of aminoPVA USPIO NPs was confirmed in HT-29 cells by transmission electron microscopy that detected the iron oxide core.AminoPVA USPIO NPs invaded three-dimensional spheroids of both HT-29 and Caco-2 cells, whereas oleic acid-coated USPIO NPs could only invade Caco-2 spheroids.Neither cationic aminoPVA USPIO NPs nor anionic oleic acid-coated USPIO NPs were transported at detectable levels across the tight CacoReady™ intestinal barrier model or the more permeable mucus-secreting CacoGoblet™ model.

View Article: PubMed Central - PubMed

Affiliation: Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.

ABSTRACT
Nanoparticles (NPs) are in clinical use or under development for therapeutic imaging and drug delivery. However, relatively little information exists concerning the uptake and transport of NPs across human colon cell layers, or their potential to invade three-dimensional models of human colon cells that better mimic the tissue structures of normal and tumoral colon. In order to gain such information, the interactions of biocompatible ultrasmall superparamagnetic iron oxide nanoparticles (USPIO NPs) (iron oxide core 9-10 nm) coated with either cationic polyvinylamine (aminoPVA) or anionic oleic acid with human HT-29 and Caco-2 colon cells was determined. The uptake of the cationic USPIO NPs was much higher than the uptake of the anionic USPIO NPs. The intracellular localization of aminoPVA USPIO NPs was confirmed in HT-29 cells by transmission electron microscopy that detected the iron oxide core. AminoPVA USPIO NPs invaded three-dimensional spheroids of both HT-29 and Caco-2 cells, whereas oleic acid-coated USPIO NPs could only invade Caco-2 spheroids. Neither cationic aminoPVA USPIO NPs nor anionic oleic acid-coated USPIO NPs were transported at detectable levels across the tight CacoReady™ intestinal barrier model or the more permeable mucus-secreting CacoGoblet™ model.

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Cytotoxicity of aminoPVA- and oleic acid-coated USPIO NPs for colon carcinoma cells. Caco-2 cells (black line) and HT-29 cells (grey line) were exposed for 24 hours to aminoPVA-coated (A) and oleic acid-coated (B) USPIO NPs, then an MTT test was performed to ascertain cytotoxicity.Notes: Means ± standard deviation were calculated, and statistical significance was assessed using Student’s t-test; *P < 0.05, exposed cells compared to control cellsAbbreviations: aminoPVA, polyvinylamine; USPIO NPs, ultrasmall superparamagnetic iron oxide nanoparticles.
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f3-ijn-7-1275: Cytotoxicity of aminoPVA- and oleic acid-coated USPIO NPs for colon carcinoma cells. Caco-2 cells (black line) and HT-29 cells (grey line) were exposed for 24 hours to aminoPVA-coated (A) and oleic acid-coated (B) USPIO NPs, then an MTT test was performed to ascertain cytotoxicity.Notes: Means ± standard deviation were calculated, and statistical significance was assessed using Student’s t-test; *P < 0.05, exposed cells compared to control cellsAbbreviations: aminoPVA, polyvinylamine; USPIO NPs, ultrasmall superparamagnetic iron oxide nanoparticles.

Mentions: An MTT test was used to evaluate cell metabolic activity to determine whether uptake resulted in a cytotoxic effect for the cells. Neither aminoPVA nor oleic acid-coated USPIO NPs inhibited cell metabolic activity except for the highest concentrations of oleic acid-coated USPIO NPs tested (Figure 3A and B) in both cell lines after 24 hours of exposure.


Evaluation of uptake and transport of cationic and anionic ultrasmall iron oxide nanoparticles by human colon cells.

Kenzaoui BH, Vilà MR, Miquel JM, Cengelli F, Juillerat-Jeanneret L - Int J Nanomedicine (2012)

Cytotoxicity of aminoPVA- and oleic acid-coated USPIO NPs for colon carcinoma cells. Caco-2 cells (black line) and HT-29 cells (grey line) were exposed for 24 hours to aminoPVA-coated (A) and oleic acid-coated (B) USPIO NPs, then an MTT test was performed to ascertain cytotoxicity.Notes: Means ± standard deviation were calculated, and statistical significance was assessed using Student’s t-test; *P < 0.05, exposed cells compared to control cellsAbbreviations: aminoPVA, polyvinylamine; USPIO NPs, ultrasmall superparamagnetic iron oxide nanoparticles.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3298390&req=5

f3-ijn-7-1275: Cytotoxicity of aminoPVA- and oleic acid-coated USPIO NPs for colon carcinoma cells. Caco-2 cells (black line) and HT-29 cells (grey line) were exposed for 24 hours to aminoPVA-coated (A) and oleic acid-coated (B) USPIO NPs, then an MTT test was performed to ascertain cytotoxicity.Notes: Means ± standard deviation were calculated, and statistical significance was assessed using Student’s t-test; *P < 0.05, exposed cells compared to control cellsAbbreviations: aminoPVA, polyvinylamine; USPIO NPs, ultrasmall superparamagnetic iron oxide nanoparticles.
Mentions: An MTT test was used to evaluate cell metabolic activity to determine whether uptake resulted in a cytotoxic effect for the cells. Neither aminoPVA nor oleic acid-coated USPIO NPs inhibited cell metabolic activity except for the highest concentrations of oleic acid-coated USPIO NPs tested (Figure 3A and B) in both cell lines after 24 hours of exposure.

Bottom Line: The intracellular localization of aminoPVA USPIO NPs was confirmed in HT-29 cells by transmission electron microscopy that detected the iron oxide core.AminoPVA USPIO NPs invaded three-dimensional spheroids of both HT-29 and Caco-2 cells, whereas oleic acid-coated USPIO NPs could only invade Caco-2 spheroids.Neither cationic aminoPVA USPIO NPs nor anionic oleic acid-coated USPIO NPs were transported at detectable levels across the tight CacoReady™ intestinal barrier model or the more permeable mucus-secreting CacoGoblet™ model.

View Article: PubMed Central - PubMed

Affiliation: Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.

ABSTRACT
Nanoparticles (NPs) are in clinical use or under development for therapeutic imaging and drug delivery. However, relatively little information exists concerning the uptake and transport of NPs across human colon cell layers, or their potential to invade three-dimensional models of human colon cells that better mimic the tissue structures of normal and tumoral colon. In order to gain such information, the interactions of biocompatible ultrasmall superparamagnetic iron oxide nanoparticles (USPIO NPs) (iron oxide core 9-10 nm) coated with either cationic polyvinylamine (aminoPVA) or anionic oleic acid with human HT-29 and Caco-2 colon cells was determined. The uptake of the cationic USPIO NPs was much higher than the uptake of the anionic USPIO NPs. The intracellular localization of aminoPVA USPIO NPs was confirmed in HT-29 cells by transmission electron microscopy that detected the iron oxide core. AminoPVA USPIO NPs invaded three-dimensional spheroids of both HT-29 and Caco-2 cells, whereas oleic acid-coated USPIO NPs could only invade Caco-2 spheroids. Neither cationic aminoPVA USPIO NPs nor anionic oleic acid-coated USPIO NPs were transported at detectable levels across the tight CacoReady™ intestinal barrier model or the more permeable mucus-secreting CacoGoblet™ model.

Show MeSH
Related in: MedlinePlus