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Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering.

Ziv-Polat O, Skaat H, Shahar A, Margel S - Int J Nanomedicine (2012)

Bottom Line: The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF.The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds.The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat-Gan, Israel.

ABSTRACT
Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGF-conjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a three-dimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

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Phase-contrast microscope images illustrating the effect of the free and conjugated bFGF on the migration and growth of the NOM cells from the cells/free or conjugated bFGF/MCs aggregates, 18 days after the cultivation of the cell aggregates in the magnetic fibrin hydrogel scaffolds. (A) exhibits the effect of the naked γ-Fe2O3 nanoparticles (control), (B) the effect of the physically conjugated bFGF nanoparticles, (C) the effect of the covalently conjugated bFGF nanoparticles. The effect of the same concentration of the free factor as that of the conjugated bFGF, and 5 and 10 times higher is illustrated in images (D), (E), and (F), respectively.Note: The asterisks indicate some of the NOM cells/bFGF-γ-Fe2O3/MCs aggregates.Abbreviations: bFGF, basal fibroblast growth factor; MC, chitosan microcarriers; NOM, nasal olfactory mucosa.
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f9-ijn-7-1259: Phase-contrast microscope images illustrating the effect of the free and conjugated bFGF on the migration and growth of the NOM cells from the cells/free or conjugated bFGF/MCs aggregates, 18 days after the cultivation of the cell aggregates in the magnetic fibrin hydrogel scaffolds. (A) exhibits the effect of the naked γ-Fe2O3 nanoparticles (control), (B) the effect of the physically conjugated bFGF nanoparticles, (C) the effect of the covalently conjugated bFGF nanoparticles. The effect of the same concentration of the free factor as that of the conjugated bFGF, and 5 and 10 times higher is illustrated in images (D), (E), and (F), respectively.Note: The asterisks indicate some of the NOM cells/bFGF-γ-Fe2O3/MCs aggregates.Abbreviations: bFGF, basal fibroblast growth factor; MC, chitosan microcarriers; NOM, nasal olfactory mucosa.

Mentions: Figure 9 demonstrates by typical phase-contrast microscope images the effect of the free and bound-bFGF on the migration of the NOM cells from the MCs aggregates and their growth in the magnetic fibrin hydrogel scaffolds 18 days after their cultivation. Figure 9A, corresponding to the cell/γ-Fe2O3 nanoparticle/MCs aggregates, shows MCs aggregates and few cells in the fibrin gel, indicating that the attachment of the naked (nonconjugated) γ-Fe2O3 nanoparticles to the cell/MCs aggregates did not induce significant proliferation and migration of the NOM cells in the magnetic fibrin hydrogel scaffolds. On the contrary, Figure 9B and C show that the attachment of either physical or covalently bFGF-conjugated nanoparticles to the cell/MCs aggregates significantly enhanced the migration and growth of the NOM cells in the magnetic fibrin hydrogel scaffolds. The beneficial effect of the bFGF-conjugated nanoparticles on the NOM cells’ growth and migration compared to that of the free factor at the same concentration, and 5 and 10 times higher, is illustrated in Figure 9D, E and F, respectively. These figures clearly show that the number of the NOM cells migrated from the MCs aggregates to the fibrin scaffolds are substantially higher in the presence of the conjugated bFGF (Figure 9B and C). A similar effect as the covalently bound factor was observed only when the free bFGF was added at a 10 times higher concentration as compared to the bound factor (Figure 9F).


Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering.

Ziv-Polat O, Skaat H, Shahar A, Margel S - Int J Nanomedicine (2012)

Phase-contrast microscope images illustrating the effect of the free and conjugated bFGF on the migration and growth of the NOM cells from the cells/free or conjugated bFGF/MCs aggregates, 18 days after the cultivation of the cell aggregates in the magnetic fibrin hydrogel scaffolds. (A) exhibits the effect of the naked γ-Fe2O3 nanoparticles (control), (B) the effect of the physically conjugated bFGF nanoparticles, (C) the effect of the covalently conjugated bFGF nanoparticles. The effect of the same concentration of the free factor as that of the conjugated bFGF, and 5 and 10 times higher is illustrated in images (D), (E), and (F), respectively.Note: The asterisks indicate some of the NOM cells/bFGF-γ-Fe2O3/MCs aggregates.Abbreviations: bFGF, basal fibroblast growth factor; MC, chitosan microcarriers; NOM, nasal olfactory mucosa.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3298389&req=5

f9-ijn-7-1259: Phase-contrast microscope images illustrating the effect of the free and conjugated bFGF on the migration and growth of the NOM cells from the cells/free or conjugated bFGF/MCs aggregates, 18 days after the cultivation of the cell aggregates in the magnetic fibrin hydrogel scaffolds. (A) exhibits the effect of the naked γ-Fe2O3 nanoparticles (control), (B) the effect of the physically conjugated bFGF nanoparticles, (C) the effect of the covalently conjugated bFGF nanoparticles. The effect of the same concentration of the free factor as that of the conjugated bFGF, and 5 and 10 times higher is illustrated in images (D), (E), and (F), respectively.Note: The asterisks indicate some of the NOM cells/bFGF-γ-Fe2O3/MCs aggregates.Abbreviations: bFGF, basal fibroblast growth factor; MC, chitosan microcarriers; NOM, nasal olfactory mucosa.
Mentions: Figure 9 demonstrates by typical phase-contrast microscope images the effect of the free and bound-bFGF on the migration of the NOM cells from the MCs aggregates and their growth in the magnetic fibrin hydrogel scaffolds 18 days after their cultivation. Figure 9A, corresponding to the cell/γ-Fe2O3 nanoparticle/MCs aggregates, shows MCs aggregates and few cells in the fibrin gel, indicating that the attachment of the naked (nonconjugated) γ-Fe2O3 nanoparticles to the cell/MCs aggregates did not induce significant proliferation and migration of the NOM cells in the magnetic fibrin hydrogel scaffolds. On the contrary, Figure 9B and C show that the attachment of either physical or covalently bFGF-conjugated nanoparticles to the cell/MCs aggregates significantly enhanced the migration and growth of the NOM cells in the magnetic fibrin hydrogel scaffolds. The beneficial effect of the bFGF-conjugated nanoparticles on the NOM cells’ growth and migration compared to that of the free factor at the same concentration, and 5 and 10 times higher, is illustrated in Figure 9D, E and F, respectively. These figures clearly show that the number of the NOM cells migrated from the MCs aggregates to the fibrin scaffolds are substantially higher in the presence of the conjugated bFGF (Figure 9B and C). A similar effect as the covalently bound factor was observed only when the free bFGF was added at a 10 times higher concentration as compared to the bound factor (Figure 9F).

Bottom Line: The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF.The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds.The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat-Gan, Israel.

ABSTRACT
Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGF-conjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a three-dimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

Show MeSH
Related in: MedlinePlus