Limits...
Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering.

Ziv-Polat O, Skaat H, Shahar A, Margel S - Int J Nanomedicine (2012)

Bottom Line: The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF.The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds.The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat-Gan, Israel.

ABSTRACT
Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGF-conjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a three-dimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

Show MeSH

Related in: MedlinePlus

Attraction of the magnetic fibrin hydrogel scaffolds containing 0.15% (top pictures) or 1.5% (bottom pictures) of the γ-Fe2O3 nanoparticles to a magnet. The top pictures represent the magnetic fibrin hydrogel scaffolds containing the thrombin-conjugated γ-Fe2O3 nanoparticles only. The bottom pictures represent the magnetic fibrin hydrogel scaffolds containing in addition to the thrombin conjugated nanoparticles also the bFGF conjugated γ-Fe2O3 nanoparticles. The arrows point to the magnetic fibrin scaffolds.Abbreviation: bFGF, basal fibroblast growth factor.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3298389&req=5

f7-ijn-7-1259: Attraction of the magnetic fibrin hydrogel scaffolds containing 0.15% (top pictures) or 1.5% (bottom pictures) of the γ-Fe2O3 nanoparticles to a magnet. The top pictures represent the magnetic fibrin hydrogel scaffolds containing the thrombin-conjugated γ-Fe2O3 nanoparticles only. The bottom pictures represent the magnetic fibrin hydrogel scaffolds containing in addition to the thrombin conjugated nanoparticles also the bFGF conjugated γ-Fe2O3 nanoparticles. The arrows point to the magnetic fibrin scaffolds.Abbreviation: bFGF, basal fibroblast growth factor.

Mentions: The magnetic properties of the fibrin hydrogel scaffolds were examined by VSM (Figure 6). Figure 6A exhibits the magnetic properties of the fibrin hydrogel scaffolds containing 0.15 weight% iron oxide nanoparticles, prepared by the interaction of thrombin-conjugated γ-Fe2O3 nanoparticles with fibrinogen, as described in the experimental section. Figure 6B exhibits the magnetic properties of the fibrin hydrogel scaffolds containing 1.5 weight% of the iron oxide nanoparticles. These scaffolds were prepared by the addition of bFGF-conjugated γ-Fe2O3 nanoparticles to the magnetic fibrin scaffolds before their clotting, as described in the experimental section. The magnetization curves shown in Figure 6 illustrate that at room temperature both M(H) plots reach saturation around 2000 Oe. The saturation magnetizations of the fibrin scaffolds containing 0.15 (A) and 1.5 (B) weight% of the iron oxide nanoparticles are 0.05 and 0.5 emu/g, respectively. The 10-fold increase in the magnetization of these scaffolds is probably attributed to the 10-fold increase in concentration of the iron oxide nanoparticles within the scaffolds. In other words, the magnetization is proportional to the concentrations of the iron oxide nanoparticles within the scaffolds: the higher the concentration the higher is the saturation magnetization. The magnetic properties of the fibrin hydrogel scaffolds were also demonstrated in a simple way, by attracting both scaffolds described above to a common magnet, as shown in Figure 7.


Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering.

Ziv-Polat O, Skaat H, Shahar A, Margel S - Int J Nanomedicine (2012)

Attraction of the magnetic fibrin hydrogel scaffolds containing 0.15% (top pictures) or 1.5% (bottom pictures) of the γ-Fe2O3 nanoparticles to a magnet. The top pictures represent the magnetic fibrin hydrogel scaffolds containing the thrombin-conjugated γ-Fe2O3 nanoparticles only. The bottom pictures represent the magnetic fibrin hydrogel scaffolds containing in addition to the thrombin conjugated nanoparticles also the bFGF conjugated γ-Fe2O3 nanoparticles. The arrows point to the magnetic fibrin scaffolds.Abbreviation: bFGF, basal fibroblast growth factor.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3298389&req=5

f7-ijn-7-1259: Attraction of the magnetic fibrin hydrogel scaffolds containing 0.15% (top pictures) or 1.5% (bottom pictures) of the γ-Fe2O3 nanoparticles to a magnet. The top pictures represent the magnetic fibrin hydrogel scaffolds containing the thrombin-conjugated γ-Fe2O3 nanoparticles only. The bottom pictures represent the magnetic fibrin hydrogel scaffolds containing in addition to the thrombin conjugated nanoparticles also the bFGF conjugated γ-Fe2O3 nanoparticles. The arrows point to the magnetic fibrin scaffolds.Abbreviation: bFGF, basal fibroblast growth factor.
Mentions: The magnetic properties of the fibrin hydrogel scaffolds were examined by VSM (Figure 6). Figure 6A exhibits the magnetic properties of the fibrin hydrogel scaffolds containing 0.15 weight% iron oxide nanoparticles, prepared by the interaction of thrombin-conjugated γ-Fe2O3 nanoparticles with fibrinogen, as described in the experimental section. Figure 6B exhibits the magnetic properties of the fibrin hydrogel scaffolds containing 1.5 weight% of the iron oxide nanoparticles. These scaffolds were prepared by the addition of bFGF-conjugated γ-Fe2O3 nanoparticles to the magnetic fibrin scaffolds before their clotting, as described in the experimental section. The magnetization curves shown in Figure 6 illustrate that at room temperature both M(H) plots reach saturation around 2000 Oe. The saturation magnetizations of the fibrin scaffolds containing 0.15 (A) and 1.5 (B) weight% of the iron oxide nanoparticles are 0.05 and 0.5 emu/g, respectively. The 10-fold increase in the magnetization of these scaffolds is probably attributed to the 10-fold increase in concentration of the iron oxide nanoparticles within the scaffolds. In other words, the magnetization is proportional to the concentrations of the iron oxide nanoparticles within the scaffolds: the higher the concentration the higher is the saturation magnetization. The magnetic properties of the fibrin hydrogel scaffolds were also demonstrated in a simple way, by attracting both scaffolds described above to a common magnet, as shown in Figure 7.

Bottom Line: The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF.The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds.The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat-Gan, Israel.

ABSTRACT
Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGF-conjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a three-dimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

Show MeSH
Related in: MedlinePlus