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Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering.

Ziv-Polat O, Skaat H, Shahar A, Margel S - Int J Nanomedicine (2012)

Bottom Line: The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF.The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds.The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat-Gan, Israel.

ABSTRACT
Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGF-conjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a three-dimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

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TEM (A) and SEM (B and C) images of the magnetic fibrin hydrogel scaffolds in the absence (A and B) or presence (C) of NOM cells. The magnetic fibrin scaffolds were prepared by the interaction of thrombin-conjugated γ-Fe2O3 nanoparticles with fibrinogen as described in the experimental part. The arrows in (A) point to the thrombin-conjugated nanoparticles.Abbreviations: NOM, nasal olfactory mucosa; SEM, scanning electron microscopy; TEM, transmission electron microscopy.
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f5-ijn-7-1259: TEM (A) and SEM (B and C) images of the magnetic fibrin hydrogel scaffolds in the absence (A and B) or presence (C) of NOM cells. The magnetic fibrin scaffolds were prepared by the interaction of thrombin-conjugated γ-Fe2O3 nanoparticles with fibrinogen as described in the experimental part. The arrows in (A) point to the thrombin-conjugated nanoparticles.Abbreviations: NOM, nasal olfactory mucosa; SEM, scanning electron microscopy; TEM, transmission electron microscopy.

Mentions: The magnetic fibrin hydrogel scaffolds are biodegradable. The duration of the degradability can be vary between a few days up to a few months, depending on various factors, eg, the density of seeded cells as well as the optimal concentrations of fibrinogen, thrombin, Ca++ ions, factor XIII, and aprotinin (a proteases inhibitor that slows down the degradation of the fibrin scaffold).50 The concentrations of these components also affect cell proliferation, migration, and differentiation within the fibrin scaffolds.47,50,51 In experiments that lasted over a month, we illustrated that our developed fibrin scaffolds containing the NOM cells in it are stable. During that time the NOM cells were grown in the scaffolds, proliferated and differentiated into neuron-like bipolar cells organized in 3D interconnected networks, as shown by SEM image (Figure 5), and later by phase-contrast microscopy (Figure 11).


Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering.

Ziv-Polat O, Skaat H, Shahar A, Margel S - Int J Nanomedicine (2012)

TEM (A) and SEM (B and C) images of the magnetic fibrin hydrogel scaffolds in the absence (A and B) or presence (C) of NOM cells. The magnetic fibrin scaffolds were prepared by the interaction of thrombin-conjugated γ-Fe2O3 nanoparticles with fibrinogen as described in the experimental part. The arrows in (A) point to the thrombin-conjugated nanoparticles.Abbreviations: NOM, nasal olfactory mucosa; SEM, scanning electron microscopy; TEM, transmission electron microscopy.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3298389&req=5

f5-ijn-7-1259: TEM (A) and SEM (B and C) images of the magnetic fibrin hydrogel scaffolds in the absence (A and B) or presence (C) of NOM cells. The magnetic fibrin scaffolds were prepared by the interaction of thrombin-conjugated γ-Fe2O3 nanoparticles with fibrinogen as described in the experimental part. The arrows in (A) point to the thrombin-conjugated nanoparticles.Abbreviations: NOM, nasal olfactory mucosa; SEM, scanning electron microscopy; TEM, transmission electron microscopy.
Mentions: The magnetic fibrin hydrogel scaffolds are biodegradable. The duration of the degradability can be vary between a few days up to a few months, depending on various factors, eg, the density of seeded cells as well as the optimal concentrations of fibrinogen, thrombin, Ca++ ions, factor XIII, and aprotinin (a proteases inhibitor that slows down the degradation of the fibrin scaffold).50 The concentrations of these components also affect cell proliferation, migration, and differentiation within the fibrin scaffolds.47,50,51 In experiments that lasted over a month, we illustrated that our developed fibrin scaffolds containing the NOM cells in it are stable. During that time the NOM cells were grown in the scaffolds, proliferated and differentiated into neuron-like bipolar cells organized in 3D interconnected networks, as shown by SEM image (Figure 5), and later by phase-contrast microscopy (Figure 11).

Bottom Line: The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF.The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds.The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat-Gan, Israel.

ABSTRACT
Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGF-conjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a three-dimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

Show MeSH
Related in: MedlinePlus