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Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering.

Ziv-Polat O, Skaat H, Shahar A, Margel S - Int J Nanomedicine (2012)

Bottom Line: The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF.The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds.The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat-Gan, Israel.

ABSTRACT
Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGF-conjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a three-dimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

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Quantitative analysis of the NOM cells adhered to the magnetic fibrin scaffold coating, or PLL coating, or uncoated culture plate (control) at different time intervals post-seeding. NOM cells were seeded in 24-well culture plates coated with the magnetic fibrin hydrogel or with PLL, or uncoated wells. 2, 4, 6, and 24 h after the seeding the wells were rinsed with the culture medium to remove the non-adherent cells. Quantification of the number of the adherent cells was performed by phase-contrast microscope images of five random non-overlapping fields of each well. Cells were then counted using ImageJ software, and the average and the standard deviation were calculated.Abbreviations: NOM, nasal olfactory mucosa; PLL, poly-L-lysine.
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f4-ijn-7-1259: Quantitative analysis of the NOM cells adhered to the magnetic fibrin scaffold coating, or PLL coating, or uncoated culture plate (control) at different time intervals post-seeding. NOM cells were seeded in 24-well culture plates coated with the magnetic fibrin hydrogel or with PLL, or uncoated wells. 2, 4, 6, and 24 h after the seeding the wells were rinsed with the culture medium to remove the non-adherent cells. Quantification of the number of the adherent cells was performed by phase-contrast microscope images of five random non-overlapping fields of each well. Cells were then counted using ImageJ software, and the average and the standard deviation were calculated.Abbreviations: NOM, nasal olfactory mucosa; PLL, poly-L-lysine.

Mentions: To illustrate the efficiency of the magnetic fibrin hydrogel scaffolds as a matrix for cell adhesion, purified NOM cells were seeded on top of the fibrin hydrogel scaffolds, in comparison to their seeding on PLL-coated dishes or noncoated culture plates (control). Figure 4 demonstrates that the magnetic fibrin hydrogel scaffolds promoted the adhesion of the NOM cells significantly better than the PLL and the noncoated control surfaces (P < 0.01). These measurements indicate that in 2, 4, 6, and 24 hours post-seeding, the amount of cells adhering to the fibrin scaffolds was 2.7, 3.0, 3.4, and 3.6 times higher than that obtained for the PLL group, and 6.2, 3.3, 3.4, and 3.6 times higher than that of the control group, respectively. The advantage of the magnetic fibrin hydrogel scaffolds for cell adhesion is probably due to the fibrin component, which naturally contains sites for cell binding. Therefore, it has been investigated as a substrate for cell adhesion, spreading, migration, and proliferation.5,6 It should be noted that the cell adhesion capacity of the scaffolds can be enhanced by the integration of adhesive molecules such as laminin and fibronectin within the scaffolds.3


Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering.

Ziv-Polat O, Skaat H, Shahar A, Margel S - Int J Nanomedicine (2012)

Quantitative analysis of the NOM cells adhered to the magnetic fibrin scaffold coating, or PLL coating, or uncoated culture plate (control) at different time intervals post-seeding. NOM cells were seeded in 24-well culture plates coated with the magnetic fibrin hydrogel or with PLL, or uncoated wells. 2, 4, 6, and 24 h after the seeding the wells were rinsed with the culture medium to remove the non-adherent cells. Quantification of the number of the adherent cells was performed by phase-contrast microscope images of five random non-overlapping fields of each well. Cells were then counted using ImageJ software, and the average and the standard deviation were calculated.Abbreviations: NOM, nasal olfactory mucosa; PLL, poly-L-lysine.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3298389&req=5

f4-ijn-7-1259: Quantitative analysis of the NOM cells adhered to the magnetic fibrin scaffold coating, or PLL coating, or uncoated culture plate (control) at different time intervals post-seeding. NOM cells were seeded in 24-well culture plates coated with the magnetic fibrin hydrogel or with PLL, or uncoated wells. 2, 4, 6, and 24 h after the seeding the wells were rinsed with the culture medium to remove the non-adherent cells. Quantification of the number of the adherent cells was performed by phase-contrast microscope images of five random non-overlapping fields of each well. Cells were then counted using ImageJ software, and the average and the standard deviation were calculated.Abbreviations: NOM, nasal olfactory mucosa; PLL, poly-L-lysine.
Mentions: To illustrate the efficiency of the magnetic fibrin hydrogel scaffolds as a matrix for cell adhesion, purified NOM cells were seeded on top of the fibrin hydrogel scaffolds, in comparison to their seeding on PLL-coated dishes or noncoated culture plates (control). Figure 4 demonstrates that the magnetic fibrin hydrogel scaffolds promoted the adhesion of the NOM cells significantly better than the PLL and the noncoated control surfaces (P < 0.01). These measurements indicate that in 2, 4, 6, and 24 hours post-seeding, the amount of cells adhering to the fibrin scaffolds was 2.7, 3.0, 3.4, and 3.6 times higher than that obtained for the PLL group, and 6.2, 3.3, 3.4, and 3.6 times higher than that of the control group, respectively. The advantage of the magnetic fibrin hydrogel scaffolds for cell adhesion is probably due to the fibrin component, which naturally contains sites for cell binding. Therefore, it has been investigated as a substrate for cell adhesion, spreading, migration, and proliferation.5,6 It should be noted that the cell adhesion capacity of the scaffolds can be enhanced by the integration of adhesive molecules such as laminin and fibronectin within the scaffolds.3

Bottom Line: The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF.The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds.The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat-Gan, Israel.

ABSTRACT
Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGF-conjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a three-dimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

Show MeSH
Related in: MedlinePlus