Limits...
Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering.

Ziv-Polat O, Skaat H, Shahar A, Margel S - Int J Nanomedicine (2012)

Bottom Line: The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF.The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds.The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat-Gan, Israel.

ABSTRACT
Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGF-conjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a three-dimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

Show MeSH

Related in: MedlinePlus

Quantitative analysis of the NOM cells migrated from the cells/nonconjugated or bFGF-conjugated nanoparticles/MCs aggregates and from the cells/different concentrations of the free factor/MCs aggregates (same concentration as the conjugated factor and 5 and 10 times higher), 5, 11, and 18 days after the cultivation of the cell aggregates in the magnetic fibrin hydrogel scaffolds. The reported values are an average of measurements performed on at least three randomly nonoverlapping fields of each triplicate tested culture.Note: ~ and – are symbols for physical and covalent bindings, respectively, of the bFGF to the γ-Fe2O3 nanoparticles.Abbreviations: bFGF, basal fibroblast growth factor; MC, chitosan microcarriers; NOM, nasal olfactory mucosa.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3298389&req=5

f10-ijn-7-1259: Quantitative analysis of the NOM cells migrated from the cells/nonconjugated or bFGF-conjugated nanoparticles/MCs aggregates and from the cells/different concentrations of the free factor/MCs aggregates (same concentration as the conjugated factor and 5 and 10 times higher), 5, 11, and 18 days after the cultivation of the cell aggregates in the magnetic fibrin hydrogel scaffolds. The reported values are an average of measurements performed on at least three randomly nonoverlapping fields of each triplicate tested culture.Note: ~ and – are symbols for physical and covalent bindings, respectively, of the bFGF to the γ-Fe2O3 nanoparticles.Abbreviations: bFGF, basal fibroblast growth factor; MC, chitosan microcarriers; NOM, nasal olfactory mucosa.

Mentions: For a quantitative determination of the effect of free/ conjugated bFGF on the kinetics of the migration of the NOM cells from the MCs aggregates to the magnetic fibrin hydrogel scaffolds, the total number of cells in the fibrin scaffolds was counted using the ImageJ software. Figure 10 illustrates the total number of cells migrated from the cell/ nonconjugated or bFGF-conjugated nanoparticle/MCs aggregates and from the cell/free bFGFs of different concentration/ MCs aggregates to the magnetic fibrin scaffolds, 5, 11, and 18 days after cultivation in the magnetic fibrin scaffolds. As expected, this figure illustrates, for all cultures, an increasing number of NOM cells in the fibrin scaffolds as the time of cultivation increased. This figure also illustrates that the migration rate of the NOM cells from the MCs aggregates and their growth in the magnetic fibrin hydrogel scaffolds is according to the following order: γ-Fe2O3-bFGF slightly higher than the γ-Fe2O3~bFGF (P > 0.05), while both are significantly higher than the γ-Fe2O3 (P < 0.05). For example, 18 days after cultivation, the number of cells counted in the presence of the γ-Fe2O3-bFGF, γ-Fe2O3~bFGF, and γ-Fe2O3 nanoparticles was 257 ± 29, 204 ± 16, and 31 ± 6 cells/ field, respectively. Figure 10 also exhibits, as expected, the acceleration in the NOM cells’ migration and growth as the concentration of the free factor increases. However, the cell migration and growth rate in the presence of the free factor at the same concentration as the conjugated factor (free ×1), or even five times higher (×5), was significantly lower than that observed for the conjugated factor (P < 0.05). Only when the concentration of the free factor was 10 times higher than that of the conjugated factor (×10), similar cell migration rates were observed (P > 0.05). For example, 18 days after cultivation, the number of cells counted in the presence of the covalently-conjugated bFGF, free ×1, free ×5, and free ×10 was 257 ± 29, 84 ± 15, 124 ± 11, and 237 ± 14 cells/ field, respectively.


Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering.

Ziv-Polat O, Skaat H, Shahar A, Margel S - Int J Nanomedicine (2012)

Quantitative analysis of the NOM cells migrated from the cells/nonconjugated or bFGF-conjugated nanoparticles/MCs aggregates and from the cells/different concentrations of the free factor/MCs aggregates (same concentration as the conjugated factor and 5 and 10 times higher), 5, 11, and 18 days after the cultivation of the cell aggregates in the magnetic fibrin hydrogel scaffolds. The reported values are an average of measurements performed on at least three randomly nonoverlapping fields of each triplicate tested culture.Note: ~ and – are symbols for physical and covalent bindings, respectively, of the bFGF to the γ-Fe2O3 nanoparticles.Abbreviations: bFGF, basal fibroblast growth factor; MC, chitosan microcarriers; NOM, nasal olfactory mucosa.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3298389&req=5

f10-ijn-7-1259: Quantitative analysis of the NOM cells migrated from the cells/nonconjugated or bFGF-conjugated nanoparticles/MCs aggregates and from the cells/different concentrations of the free factor/MCs aggregates (same concentration as the conjugated factor and 5 and 10 times higher), 5, 11, and 18 days after the cultivation of the cell aggregates in the magnetic fibrin hydrogel scaffolds. The reported values are an average of measurements performed on at least three randomly nonoverlapping fields of each triplicate tested culture.Note: ~ and – are symbols for physical and covalent bindings, respectively, of the bFGF to the γ-Fe2O3 nanoparticles.Abbreviations: bFGF, basal fibroblast growth factor; MC, chitosan microcarriers; NOM, nasal olfactory mucosa.
Mentions: For a quantitative determination of the effect of free/ conjugated bFGF on the kinetics of the migration of the NOM cells from the MCs aggregates to the magnetic fibrin hydrogel scaffolds, the total number of cells in the fibrin scaffolds was counted using the ImageJ software. Figure 10 illustrates the total number of cells migrated from the cell/ nonconjugated or bFGF-conjugated nanoparticle/MCs aggregates and from the cell/free bFGFs of different concentration/ MCs aggregates to the magnetic fibrin scaffolds, 5, 11, and 18 days after cultivation in the magnetic fibrin scaffolds. As expected, this figure illustrates, for all cultures, an increasing number of NOM cells in the fibrin scaffolds as the time of cultivation increased. This figure also illustrates that the migration rate of the NOM cells from the MCs aggregates and their growth in the magnetic fibrin hydrogel scaffolds is according to the following order: γ-Fe2O3-bFGF slightly higher than the γ-Fe2O3~bFGF (P > 0.05), while both are significantly higher than the γ-Fe2O3 (P < 0.05). For example, 18 days after cultivation, the number of cells counted in the presence of the γ-Fe2O3-bFGF, γ-Fe2O3~bFGF, and γ-Fe2O3 nanoparticles was 257 ± 29, 204 ± 16, and 31 ± 6 cells/ field, respectively. Figure 10 also exhibits, as expected, the acceleration in the NOM cells’ migration and growth as the concentration of the free factor increases. However, the cell migration and growth rate in the presence of the free factor at the same concentration as the conjugated factor (free ×1), or even five times higher (×5), was significantly lower than that observed for the conjugated factor (P < 0.05). Only when the concentration of the free factor was 10 times higher than that of the conjugated factor (×10), similar cell migration rates were observed (P > 0.05). For example, 18 days after cultivation, the number of cells counted in the presence of the covalently-conjugated bFGF, free ×1, free ×5, and free ×10 was 257 ± 29, 84 ± 15, 124 ± 11, and 237 ± 14 cells/ field, respectively.

Bottom Line: The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF.The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds.The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat-Gan, Israel.

ABSTRACT
Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGF-conjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a three-dimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

Show MeSH
Related in: MedlinePlus