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Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering.

Ziv-Polat O, Skaat H, Shahar A, Margel S - Int J Nanomedicine (2012)

Bottom Line: The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF.The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds.The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat-Gan, Israel.

ABSTRACT
Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGF-conjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a three-dimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

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Physical and covalent conjugation of thrombin and bFGF to the γ-Fe2O3 nanoparticles. ≈, ~, and – are symbols for precipitation, physical binding and covalent binding of various ligands to the γ-Fe2O3 nanoparticles, respectively.Abbreviation: bFGF, basal fibroblast growth factor.
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f1-ijn-7-1259: Physical and covalent conjugation of thrombin and bFGF to the γ-Fe2O3 nanoparticles. ≈, ~, and – are symbols for precipitation, physical binding and covalent binding of various ligands to the γ-Fe2O3 nanoparticles, respectively.Abbreviation: bFGF, basal fibroblast growth factor.

Mentions: Figure 1 describes the general scheme through which the physical conjugation of thrombin and the physical and covalent conjugation of bFGF onto the surface of the γ-Fe2O3 nanoparticles were performed. Figure 1 illustrates that the covalent conjugation of bFGF to the γ-Fe2O3 nanoparticles is based on the presence of gelatin thin layer on the surface of these nanoparticles, as shown in Figure 2A and B and described previously.44 The surface gelatin provides functional groups, eg, primary amines and hydroxyls, through which functionalization of these nanoparticles with activated double bonds, via the Michael addition reaction, was accomplished with excess DVS. The residual activated double bonds of the γ-Fe2O3–DVS nanoparticles were then used for covalent binding of the bFGF to the surface of the nanoparticles, again via the Michael addition reaction. Blocking of the remaining double bonds of the γ-Fe2O3–bFGF nanoparticles was done with glycine, according to the experimental part.


Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering.

Ziv-Polat O, Skaat H, Shahar A, Margel S - Int J Nanomedicine (2012)

Physical and covalent conjugation of thrombin and bFGF to the γ-Fe2O3 nanoparticles. ≈, ~, and – are symbols for precipitation, physical binding and covalent binding of various ligands to the γ-Fe2O3 nanoparticles, respectively.Abbreviation: bFGF, basal fibroblast growth factor.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3298389&req=5

f1-ijn-7-1259: Physical and covalent conjugation of thrombin and bFGF to the γ-Fe2O3 nanoparticles. ≈, ~, and – are symbols for precipitation, physical binding and covalent binding of various ligands to the γ-Fe2O3 nanoparticles, respectively.Abbreviation: bFGF, basal fibroblast growth factor.
Mentions: Figure 1 describes the general scheme through which the physical conjugation of thrombin and the physical and covalent conjugation of bFGF onto the surface of the γ-Fe2O3 nanoparticles were performed. Figure 1 illustrates that the covalent conjugation of bFGF to the γ-Fe2O3 nanoparticles is based on the presence of gelatin thin layer on the surface of these nanoparticles, as shown in Figure 2A and B and described previously.44 The surface gelatin provides functional groups, eg, primary amines and hydroxyls, through which functionalization of these nanoparticles with activated double bonds, via the Michael addition reaction, was accomplished with excess DVS. The residual activated double bonds of the γ-Fe2O3–DVS nanoparticles were then used for covalent binding of the bFGF to the surface of the nanoparticles, again via the Michael addition reaction. Blocking of the remaining double bonds of the γ-Fe2O3–bFGF nanoparticles was done with glycine, according to the experimental part.

Bottom Line: The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF.The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds.The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat-Gan, Israel.

ABSTRACT
Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGF-conjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a three-dimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

Show MeSH
Related in: MedlinePlus