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Antibiofilm surface functionalization of catheters by magnesium fluoride nanoparticles.

Lellouche J, Friedman A, Lahmi R, Gedanken A, Banin E - Int J Nanomedicine (2012)

Bottom Line: Two bacterial strains most commonly associated with catheter infections, Escherichia coli and Staphylococcus aureus, were cultured in tryptic soy broth, artificial urine and human plasma on the modified catheters.The MgF(2) NP-coated catheters were able to significantly reduce bacterial colonization for a period of 1 week compared to the uncoated control.Taken together, our results indicate that the surface modification of catheters with MgF(2) NPs can be effective in preventing bacterial colonization and can provide catheters with long-lasting self-sterilizing properties.

View Article: PubMed Central - PubMed

Affiliation: The Mina and Everard Goodman Faculty of Life Sciences, The Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel.

ABSTRACT
The ability of bacteria to colonize catheters is a major cause of infection. In the current study, catheters were surface-modified with MgF(2) nanoparticles (NPs) using a sonochemical synthesis protocol described previously. The one-step synthesis and coating procedure yielded a homogenous MgF(2) NP layer on both the inside and outside of the catheter, as analyzed by high resolution scanning electron microscopy and energy dispersive spectroscopy. The coating thickness varied from approximately 750 nm to 1000 nm on the inner walls and from approximately 450 nm to approximately 580 nm for the outer wall. The coating consisted of spherical MgF(2) NPs with an average diameter of approximately 25 nm. These MgF(2) NP-modified catheters were investigated for their ability to restrict bacterial biofilm formation. Two bacterial strains most commonly associated with catheter infections, Escherichia coli and Staphylococcus aureus, were cultured in tryptic soy broth, artificial urine and human plasma on the modified catheters. The MgF(2) NP-coated catheters were able to significantly reduce bacterial colonization for a period of 1 week compared to the uncoated control. Finally, the potential cytotoxicity of MgF(2) NPs was also evaluated using human and mammalian cell lines and no significant reduction in the mitochondrial metabolism was observed. Taken together, our results indicate that the surface modification of catheters with MgF(2) NPs can be effective in preventing bacterial colonization and can provide catheters with long-lasting self-sterilizing properties.

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Biocompatibility of MgF2 NP-coated catheters. Left column: DIC imaging of HeLa cells grown on uncoated and MgF2 NP-coated catheters for 24 hours. Right column: Fluorescence microscopy overlay of the DNA (blue) and β-tubulin (red) of the same cells imaged by DIC.Abbreviations: DIC, differential interference contrast; NP, nanoparticle.
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f6-ijn-7-1175: Biocompatibility of MgF2 NP-coated catheters. Left column: DIC imaging of HeLa cells grown on uncoated and MgF2 NP-coated catheters for 24 hours. Right column: Fluorescence microscopy overlay of the DNA (blue) and β-tubulin (red) of the same cells imaged by DIC.Abbreviations: DIC, differential interference contrast; NP, nanoparticle.

Mentions: We confirmed these results by fluorescence microscopy using HeLa cells. As depicted in Figure 6, no changes in cell morphology, size and membrane symmetry were observed in cells grown on MgF2 NP-coated surfaces. In addition, no sign of apoptosis such as the appearance of apoptotic bodies was observed. Furthermore, fluorescent microscopy was used to evaluate a potential process of programmed cell death; β-tubulin staining showed that the cytoskeleton of cells grown on MgF2 NP-coated catheters had the same phenotype as the control cells grown on uncoated catheters (Figure 6). DNA staining was also conducted and no nuclear fragmentation, chromatin condensation, or chromosomal DNA fragmentations were observed (Figure 6). Taken together, these preliminary results support further testing of the tolerance and cytotoxicity of the MgF2 NP-coated surfaces, and emphasize the potential of using these NPs for various medical applications.


Antibiofilm surface functionalization of catheters by magnesium fluoride nanoparticles.

Lellouche J, Friedman A, Lahmi R, Gedanken A, Banin E - Int J Nanomedicine (2012)

Biocompatibility of MgF2 NP-coated catheters. Left column: DIC imaging of HeLa cells grown on uncoated and MgF2 NP-coated catheters for 24 hours. Right column: Fluorescence microscopy overlay of the DNA (blue) and β-tubulin (red) of the same cells imaged by DIC.Abbreviations: DIC, differential interference contrast; NP, nanoparticle.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3298385&req=5

f6-ijn-7-1175: Biocompatibility of MgF2 NP-coated catheters. Left column: DIC imaging of HeLa cells grown on uncoated and MgF2 NP-coated catheters for 24 hours. Right column: Fluorescence microscopy overlay of the DNA (blue) and β-tubulin (red) of the same cells imaged by DIC.Abbreviations: DIC, differential interference contrast; NP, nanoparticle.
Mentions: We confirmed these results by fluorescence microscopy using HeLa cells. As depicted in Figure 6, no changes in cell morphology, size and membrane symmetry were observed in cells grown on MgF2 NP-coated surfaces. In addition, no sign of apoptosis such as the appearance of apoptotic bodies was observed. Furthermore, fluorescent microscopy was used to evaluate a potential process of programmed cell death; β-tubulin staining showed that the cytoskeleton of cells grown on MgF2 NP-coated catheters had the same phenotype as the control cells grown on uncoated catheters (Figure 6). DNA staining was also conducted and no nuclear fragmentation, chromatin condensation, or chromosomal DNA fragmentations were observed (Figure 6). Taken together, these preliminary results support further testing of the tolerance and cytotoxicity of the MgF2 NP-coated surfaces, and emphasize the potential of using these NPs for various medical applications.

Bottom Line: Two bacterial strains most commonly associated with catheter infections, Escherichia coli and Staphylococcus aureus, were cultured in tryptic soy broth, artificial urine and human plasma on the modified catheters.The MgF(2) NP-coated catheters were able to significantly reduce bacterial colonization for a period of 1 week compared to the uncoated control.Taken together, our results indicate that the surface modification of catheters with MgF(2) NPs can be effective in preventing bacterial colonization and can provide catheters with long-lasting self-sterilizing properties.

View Article: PubMed Central - PubMed

Affiliation: The Mina and Everard Goodman Faculty of Life Sciences, The Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel.

ABSTRACT
The ability of bacteria to colonize catheters is a major cause of infection. In the current study, catheters were surface-modified with MgF(2) nanoparticles (NPs) using a sonochemical synthesis protocol described previously. The one-step synthesis and coating procedure yielded a homogenous MgF(2) NP layer on both the inside and outside of the catheter, as analyzed by high resolution scanning electron microscopy and energy dispersive spectroscopy. The coating thickness varied from approximately 750 nm to 1000 nm on the inner walls and from approximately 450 nm to approximately 580 nm for the outer wall. The coating consisted of spherical MgF(2) NPs with an average diameter of approximately 25 nm. These MgF(2) NP-modified catheters were investigated for their ability to restrict bacterial biofilm formation. Two bacterial strains most commonly associated with catheter infections, Escherichia coli and Staphylococcus aureus, were cultured in tryptic soy broth, artificial urine and human plasma on the modified catheters. The MgF(2) NP-coated catheters were able to significantly reduce bacterial colonization for a period of 1 week compared to the uncoated control. Finally, the potential cytotoxicity of MgF(2) NPs was also evaluated using human and mammalian cell lines and no significant reduction in the mitochondrial metabolism was observed. Taken together, our results indicate that the surface modification of catheters with MgF(2) NPs can be effective in preventing bacterial colonization and can provide catheters with long-lasting self-sterilizing properties.

Show MeSH
Related in: MedlinePlus