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Antibiofilm surface functionalization of catheters by magnesium fluoride nanoparticles.

Lellouche J, Friedman A, Lahmi R, Gedanken A, Banin E - Int J Nanomedicine (2012)

Bottom Line: Two bacterial strains most commonly associated with catheter infections, Escherichia coli and Staphylococcus aureus, were cultured in tryptic soy broth, artificial urine and human plasma on the modified catheters.The MgF(2) NP-coated catheters were able to significantly reduce bacterial colonization for a period of 1 week compared to the uncoated control.Taken together, our results indicate that the surface modification of catheters with MgF(2) NPs can be effective in preventing bacterial colonization and can provide catheters with long-lasting self-sterilizing properties.

View Article: PubMed Central - PubMed

Affiliation: The Mina and Everard Goodman Faculty of Life Sciences, The Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel.

ABSTRACT
The ability of bacteria to colonize catheters is a major cause of infection. In the current study, catheters were surface-modified with MgF(2) nanoparticles (NPs) using a sonochemical synthesis protocol described previously. The one-step synthesis and coating procedure yielded a homogenous MgF(2) NP layer on both the inside and outside of the catheter, as analyzed by high resolution scanning electron microscopy and energy dispersive spectroscopy. The coating thickness varied from approximately 750 nm to 1000 nm on the inner walls and from approximately 450 nm to approximately 580 nm for the outer wall. The coating consisted of spherical MgF(2) NPs with an average diameter of approximately 25 nm. These MgF(2) NP-modified catheters were investigated for their ability to restrict bacterial biofilm formation. Two bacterial strains most commonly associated with catheter infections, Escherichia coli and Staphylococcus aureus, were cultured in tryptic soy broth, artificial urine and human plasma on the modified catheters. The MgF(2) NP-coated catheters were able to significantly reduce bacterial colonization for a period of 1 week compared to the uncoated control. Finally, the potential cytotoxicity of MgF(2) NPs was also evaluated using human and mammalian cell lines and no significant reduction in the mitochondrial metabolism was observed. Taken together, our results indicate that the surface modification of catheters with MgF(2) NPs can be effective in preventing bacterial colonization and can provide catheters with long-lasting self-sterilizing properties.

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Imaging of sonochemical MgF2 NP catheter coating. Catheters were coated using a sonochemical procedure described in the experimental section. HR SEM images of the lateral sections of the internal and external walls of uncoated and MgF2 NP-coated catheters are presented. Inserts provide an enlarged view of the coating showing the typical spherical MgF2 NP structure.Note: Black arrows indicate the thickness of the MgF2 NP coating.Abbreviations: HR SEM, high resolution scanning electron microscope; NP, nanoparticle.
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f2-ijn-7-1175: Imaging of sonochemical MgF2 NP catheter coating. Catheters were coated using a sonochemical procedure described in the experimental section. HR SEM images of the lateral sections of the internal and external walls of uncoated and MgF2 NP-coated catheters are presented. Inserts provide an enlarged view of the coating showing the typical spherical MgF2 NP structure.Note: Black arrows indicate the thickness of the MgF2 NP coating.Abbreviations: HR SEM, high resolution scanning electron microscope; NP, nanoparticle.

Mentions: Our initial characterization measurements were aimed at determining the shape and the size of the MgF2 NPs formed in the sonochemical reaction. The MgF2 NP-coated catheters were imaged by HR SEM (Figure 2). The catheter surfaces were completely covered, with spherical NPs having an average size of approximately 25 nm (Figure 2). The size and the morphology are similar to the data measured by HR TEM and XRD for the suspended NPs (formed under similar reaction conditions, but without the catheters in the reaction vessel: see Figure S1).


Antibiofilm surface functionalization of catheters by magnesium fluoride nanoparticles.

Lellouche J, Friedman A, Lahmi R, Gedanken A, Banin E - Int J Nanomedicine (2012)

Imaging of sonochemical MgF2 NP catheter coating. Catheters were coated using a sonochemical procedure described in the experimental section. HR SEM images of the lateral sections of the internal and external walls of uncoated and MgF2 NP-coated catheters are presented. Inserts provide an enlarged view of the coating showing the typical spherical MgF2 NP structure.Note: Black arrows indicate the thickness of the MgF2 NP coating.Abbreviations: HR SEM, high resolution scanning electron microscope; NP, nanoparticle.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3298385&req=5

f2-ijn-7-1175: Imaging of sonochemical MgF2 NP catheter coating. Catheters were coated using a sonochemical procedure described in the experimental section. HR SEM images of the lateral sections of the internal and external walls of uncoated and MgF2 NP-coated catheters are presented. Inserts provide an enlarged view of the coating showing the typical spherical MgF2 NP structure.Note: Black arrows indicate the thickness of the MgF2 NP coating.Abbreviations: HR SEM, high resolution scanning electron microscope; NP, nanoparticle.
Mentions: Our initial characterization measurements were aimed at determining the shape and the size of the MgF2 NPs formed in the sonochemical reaction. The MgF2 NP-coated catheters were imaged by HR SEM (Figure 2). The catheter surfaces were completely covered, with spherical NPs having an average size of approximately 25 nm (Figure 2). The size and the morphology are similar to the data measured by HR TEM and XRD for the suspended NPs (formed under similar reaction conditions, but without the catheters in the reaction vessel: see Figure S1).

Bottom Line: Two bacterial strains most commonly associated with catheter infections, Escherichia coli and Staphylococcus aureus, were cultured in tryptic soy broth, artificial urine and human plasma on the modified catheters.The MgF(2) NP-coated catheters were able to significantly reduce bacterial colonization for a period of 1 week compared to the uncoated control.Taken together, our results indicate that the surface modification of catheters with MgF(2) NPs can be effective in preventing bacterial colonization and can provide catheters with long-lasting self-sterilizing properties.

View Article: PubMed Central - PubMed

Affiliation: The Mina and Everard Goodman Faculty of Life Sciences, The Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel.

ABSTRACT
The ability of bacteria to colonize catheters is a major cause of infection. In the current study, catheters were surface-modified with MgF(2) nanoparticles (NPs) using a sonochemical synthesis protocol described previously. The one-step synthesis and coating procedure yielded a homogenous MgF(2) NP layer on both the inside and outside of the catheter, as analyzed by high resolution scanning electron microscopy and energy dispersive spectroscopy. The coating thickness varied from approximately 750 nm to 1000 nm on the inner walls and from approximately 450 nm to approximately 580 nm for the outer wall. The coating consisted of spherical MgF(2) NPs with an average diameter of approximately 25 nm. These MgF(2) NP-modified catheters were investigated for their ability to restrict bacterial biofilm formation. Two bacterial strains most commonly associated with catheter infections, Escherichia coli and Staphylococcus aureus, were cultured in tryptic soy broth, artificial urine and human plasma on the modified catheters. The MgF(2) NP-coated catheters were able to significantly reduce bacterial colonization for a period of 1 week compared to the uncoated control. Finally, the potential cytotoxicity of MgF(2) NPs was also evaluated using human and mammalian cell lines and no significant reduction in the mitochondrial metabolism was observed. Taken together, our results indicate that the surface modification of catheters with MgF(2) NPs can be effective in preventing bacterial colonization and can provide catheters with long-lasting self-sterilizing properties.

Show MeSH
Related in: MedlinePlus