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Speeding up coeliac disease diagnosis in cardiological settings.

Chicco D, Taddio A, Sinagra G, Di Lenarda A, Ferrara F, Moretti M, Martelossi S, Di Toro N, Ventura A, Not T - Arch Med Sci (2010)

Bottom Line: This test was compared to the enzyme-linked immunosorbent assay and the anti-endomysium antibody test.During the gluten-free diet, the patient with the worst left ventricular ejection fraction (LVEF) underwent heart transplant, and LVEF values improved in the other two.Early detection of CD in a cardiological setting allows prompt treatment with a gluten-free diet of gluten-dependent complaints with potential benefits for the course of DCM.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Department, Azienda Ospedaliero-Universitaria di Trieste, Trieste, Italy.

ABSTRACT

Introduction: High prevalence of coeliac disease (CD) has been reported among patients with idiopathic dilated cardiomyopathy (DCM). We evaluated the feasibility and diagnostic accuracy of screening for CD by rapid test of anti-transglutaminase antibodies in the cardiology outpatients' clinic.

Material and methods: We screened the blood samples of 104 patients with DCM, 44 of their first-degree relatives, 63 diseased controls and 101 healthy controls for the presence of anti-transglutaminase antibodies in a drop of whole blood using a rapid assay. This test was compared to the enzyme-linked immunosorbent assay and the anti-endomysium antibody test.

Results: Our rapid test was positive in three (2.9%) DCM patients, in one (2%) relative and in one (1%) healthy control. These subjects were positive at both control assays. Two DCM patients had iron-deficient anaemia. The healthy relative was asymptomatic, while the healthy control experienced extreme asthenia. The relative refused intestinal biopsy, while the others showed histological evidence of CD. During the gluten-free diet, the patient with the worst left ventricular ejection fraction (LVEF) underwent heart transplant, and LVEF values improved in the other two. Anaemia and tiredness resolved in all patients.

Conclusion: Early detection of CD in a cardiological setting allows prompt treatment with a gluten-free diet of gluten-dependent complaints with potential benefits for the course of DCM.

No MeSH data available.


Related in: MedlinePlus

Positive and negative results for anti-transglutaminase antibodies using the rapid immunoassay. The first band (head-arrow) must always appear as a technical control, the second band (arrow) represents the positive result of the test. No second band was detected in the first strip incubated with a blood sample that tested negative for anti-transglutaminase antibodies. Different shades of grey bands identified coeliac patients in the second and third strip
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Figure 1: Positive and negative results for anti-transglutaminase antibodies using the rapid immunoassay. The first band (head-arrow) must always appear as a technical control, the second band (arrow) represents the positive result of the test. No second band was detected in the first strip incubated with a blood sample that tested negative for anti-transglutaminase antibodies. Different shades of grey bands identified coeliac patients in the second and third strip

Mentions: The rapid test for detecting IgA anti-tTG, based on a whole blood drop, was used as previously described [6] following the manufacturer’s instructions (Eu-tTG Quick, Eurospital, Italy). Anti-tTG were measured by nurses using sticks incubated for 5 minutes in a solution containing 10 µl of whole blood from a finger prick. Positive results appear as varying shades of grey bands on the sticks (Figure 1). The shades were interpreted by two operators blind to subjects’ histories and laboratory findings.


Speeding up coeliac disease diagnosis in cardiological settings.

Chicco D, Taddio A, Sinagra G, Di Lenarda A, Ferrara F, Moretti M, Martelossi S, Di Toro N, Ventura A, Not T - Arch Med Sci (2010)

Positive and negative results for anti-transglutaminase antibodies using the rapid immunoassay. The first band (head-arrow) must always appear as a technical control, the second band (arrow) represents the positive result of the test. No second band was detected in the first strip incubated with a blood sample that tested negative for anti-transglutaminase antibodies. Different shades of grey bands identified coeliac patients in the second and third strip
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3298342&req=5

Figure 1: Positive and negative results for anti-transglutaminase antibodies using the rapid immunoassay. The first band (head-arrow) must always appear as a technical control, the second band (arrow) represents the positive result of the test. No second band was detected in the first strip incubated with a blood sample that tested negative for anti-transglutaminase antibodies. Different shades of grey bands identified coeliac patients in the second and third strip
Mentions: The rapid test for detecting IgA anti-tTG, based on a whole blood drop, was used as previously described [6] following the manufacturer’s instructions (Eu-tTG Quick, Eurospital, Italy). Anti-tTG were measured by nurses using sticks incubated for 5 minutes in a solution containing 10 µl of whole blood from a finger prick. Positive results appear as varying shades of grey bands on the sticks (Figure 1). The shades were interpreted by two operators blind to subjects’ histories and laboratory findings.

Bottom Line: This test was compared to the enzyme-linked immunosorbent assay and the anti-endomysium antibody test.During the gluten-free diet, the patient with the worst left ventricular ejection fraction (LVEF) underwent heart transplant, and LVEF values improved in the other two.Early detection of CD in a cardiological setting allows prompt treatment with a gluten-free diet of gluten-dependent complaints with potential benefits for the course of DCM.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Department, Azienda Ospedaliero-Universitaria di Trieste, Trieste, Italy.

ABSTRACT

Introduction: High prevalence of coeliac disease (CD) has been reported among patients with idiopathic dilated cardiomyopathy (DCM). We evaluated the feasibility and diagnostic accuracy of screening for CD by rapid test of anti-transglutaminase antibodies in the cardiology outpatients' clinic.

Material and methods: We screened the blood samples of 104 patients with DCM, 44 of their first-degree relatives, 63 diseased controls and 101 healthy controls for the presence of anti-transglutaminase antibodies in a drop of whole blood using a rapid assay. This test was compared to the enzyme-linked immunosorbent assay and the anti-endomysium antibody test.

Results: Our rapid test was positive in three (2.9%) DCM patients, in one (2%) relative and in one (1%) healthy control. These subjects were positive at both control assays. Two DCM patients had iron-deficient anaemia. The healthy relative was asymptomatic, while the healthy control experienced extreme asthenia. The relative refused intestinal biopsy, while the others showed histological evidence of CD. During the gluten-free diet, the patient with the worst left ventricular ejection fraction (LVEF) underwent heart transplant, and LVEF values improved in the other two. Anaemia and tiredness resolved in all patients.

Conclusion: Early detection of CD in a cardiological setting allows prompt treatment with a gluten-free diet of gluten-dependent complaints with potential benefits for the course of DCM.

No MeSH data available.


Related in: MedlinePlus