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Picornavirus salivirus/klassevirus in children with diarrhea, China.

Shan T, Wang C, Cui L, Yu Y, Delwart E, Zhao W, Zhu C, Lan D, Dai X, Hua X - Emerging Infect. Dis. (2010)

Bottom Line: To learn more about salivirus/klassevirus, we tested feces of children with diarrhea in China during 2008-2009.We isolated the virus from 9/216 diarrhea samples and 0/96 control samples.The nearly full polyprotein of 1 isolate, SH1, showed 95% identity with a salivirus from Nigeria, indicating widespread distribution and association with diarrhea.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Jiao Tong University, Shanghai, People's Republic of China.

ABSTRACT
To learn more about salivirus/klassevirus, we tested feces of children with diarrhea in China during 2008-2009. We isolated the virus from 9/216 diarrhea samples and 0/96 control samples. The nearly full polyprotein of 1 isolate, SH1, showed 95% identity with a salivirus from Nigeria, indicating widespread distribution and association with diarrhea.

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Related in: MedlinePlus

Phylogenetic analysis of the more variable P1 region of the salivirus/klassevirus isolated from fecal samples of 9 (4.2%) of 216 children with diarrhea in the People’s Republic of China, April 2008–March 2009, and 45 representative strains. Phylogenetic tree was constructed by the neighbor-joining method with 1,000 bootstrap replicates by using MEGA4.0 software (www.megasoftware.net). Bootstrap values are indicated at each branching point. The isolate SH1 is marked with a triangle. Scale bar indicates estimated phylogenetic divergence.
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Figure 1: Phylogenetic analysis of the more variable P1 region of the salivirus/klassevirus isolated from fecal samples of 9 (4.2%) of 216 children with diarrhea in the People’s Republic of China, April 2008–March 2009, and 45 representative strains. Phylogenetic tree was constructed by the neighbor-joining method with 1,000 bootstrap replicates by using MEGA4.0 software (www.megasoftware.net). Bootstrap values are indicated at each branching point. The isolate SH1 is marked with a triangle. Scale bar indicates estimated phylogenetic divergence.

Mentions: The complete genomic sequence of strain SH1 was then determined by using 10 sets of specific oligonucleotide primers designed on the complete genome of GQ184145, GQ253930, and GQ179640. The nearly full-genome genome of this virus strain was 7,798 nt and contained an open reading frame (ORF) with a length of 7,107 nt, encoding a putative polyprotein precursor of 2,369 aa. This ORF is preceded by a 5′ UTR at least 624 nt long and followed by a 3′ UTR at least 67 nt long. Phylogenetic analysis using the more variable P1 region of SH1 and 45 representative picornaviruses (including 3 salivirus/klassevirus strain) confirmed the close relationship of this strain with strains from other continents; it was most closely related to a human klassevirus from the United States (GQ184145) (Figure).


Picornavirus salivirus/klassevirus in children with diarrhea, China.

Shan T, Wang C, Cui L, Yu Y, Delwart E, Zhao W, Zhu C, Lan D, Dai X, Hua X - Emerging Infect. Dis. (2010)

Phylogenetic analysis of the more variable P1 region of the salivirus/klassevirus isolated from fecal samples of 9 (4.2%) of 216 children with diarrhea in the People’s Republic of China, April 2008–March 2009, and 45 representative strains. Phylogenetic tree was constructed by the neighbor-joining method with 1,000 bootstrap replicates by using MEGA4.0 software (www.megasoftware.net). Bootstrap values are indicated at each branching point. The isolate SH1 is marked with a triangle. Scale bar indicates estimated phylogenetic divergence.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3298310&req=5

Figure 1: Phylogenetic analysis of the more variable P1 region of the salivirus/klassevirus isolated from fecal samples of 9 (4.2%) of 216 children with diarrhea in the People’s Republic of China, April 2008–March 2009, and 45 representative strains. Phylogenetic tree was constructed by the neighbor-joining method with 1,000 bootstrap replicates by using MEGA4.0 software (www.megasoftware.net). Bootstrap values are indicated at each branching point. The isolate SH1 is marked with a triangle. Scale bar indicates estimated phylogenetic divergence.
Mentions: The complete genomic sequence of strain SH1 was then determined by using 10 sets of specific oligonucleotide primers designed on the complete genome of GQ184145, GQ253930, and GQ179640. The nearly full-genome genome of this virus strain was 7,798 nt and contained an open reading frame (ORF) with a length of 7,107 nt, encoding a putative polyprotein precursor of 2,369 aa. This ORF is preceded by a 5′ UTR at least 624 nt long and followed by a 3′ UTR at least 67 nt long. Phylogenetic analysis using the more variable P1 region of SH1 and 45 representative picornaviruses (including 3 salivirus/klassevirus strain) confirmed the close relationship of this strain with strains from other continents; it was most closely related to a human klassevirus from the United States (GQ184145) (Figure).

Bottom Line: To learn more about salivirus/klassevirus, we tested feces of children with diarrhea in China during 2008-2009.We isolated the virus from 9/216 diarrhea samples and 0/96 control samples.The nearly full polyprotein of 1 isolate, SH1, showed 95% identity with a salivirus from Nigeria, indicating widespread distribution and association with diarrhea.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Jiao Tong University, Shanghai, People's Republic of China.

ABSTRACT
To learn more about salivirus/klassevirus, we tested feces of children with diarrhea in China during 2008-2009. We isolated the virus from 9/216 diarrhea samples and 0/96 control samples. The nearly full polyprotein of 1 isolate, SH1, showed 95% identity with a salivirus from Nigeria, indicating widespread distribution and association with diarrhea.

Show MeSH
Related in: MedlinePlus