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A miniature probe for ultrasonic penetration of a single cell.

Wu T, Zhou Z, Wang Q, Yang X, Xiao M - Sensors (Basel) (2009)

Bottom Line: This paper seeks to develop a miniature ultrasonic probe experiment system for cell penetration.A miniature ultrasonic probe is designed and optimized using the Precise Four Terminal Network Method and Finite Element Method (FEM) and an ultrasonic generator to drive the probe is designed.The system was able to successfully puncture a single fish cell.

View Article: PubMed Central - PubMed

Affiliation: MEMS Lab, Department of Precision Instruments & Mechanology, Tsinghua University, Beijing 100084, P.R. China; E-Mails: zhouzy@mail.tsinghua.edu.cn ; yangxing@mail.tsinghua.edu.cn ; xiaomf03@mails.tsinghua.edu.cn.

ABSTRACT
Although ultrasound cavitation must be avoided for safe diagnostic applications, the ability of ultrasound to disrupt cell membranes has taken on increasing significance as a method to facilitate drug and gene delivery. A new ultrasonic resonance driving method is introduced to penetrate rigid wall plant cells or oocytes with springy cell membranes. When a reasonable design is created, ultrasound can gather energy and increase the amplitude factor. Ultrasonic penetration enables exogenous materials to enter cells without damaging them by utilizing instant acceleration. This paper seeks to develop a miniature ultrasonic probe experiment system for cell penetration. A miniature ultrasonic probe is designed and optimized using the Precise Four Terminal Network Method and Finite Element Method (FEM) and an ultrasonic generator to drive the probe is designed. The system was able to successfully puncture a single fish cell.

No MeSH data available.


Related in: MedlinePlus

Four-terminal network principle.
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f3-sensors-09-03325: Four-terminal network principle.

Mentions: The Precise Four Terminal Network Method and Finite Element Simulation, with which the ultrasonic probe is designed, are introduced as follows: an ultrasonic transducer was schemed out by the four-terminal network method [13-15], which is a novel design method. Its basic principle is that a single level ultrasonic horn can be regarded as an equivalent mechanical four-terminal network. Every adjacent level is connected in series (see Figure 3).


A miniature probe for ultrasonic penetration of a single cell.

Wu T, Zhou Z, Wang Q, Yang X, Xiao M - Sensors (Basel) (2009)

Four-terminal network principle.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3297146&req=5

f3-sensors-09-03325: Four-terminal network principle.
Mentions: The Precise Four Terminal Network Method and Finite Element Simulation, with which the ultrasonic probe is designed, are introduced as follows: an ultrasonic transducer was schemed out by the four-terminal network method [13-15], which is a novel design method. Its basic principle is that a single level ultrasonic horn can be regarded as an equivalent mechanical four-terminal network. Every adjacent level is connected in series (see Figure 3).

Bottom Line: This paper seeks to develop a miniature ultrasonic probe experiment system for cell penetration.A miniature ultrasonic probe is designed and optimized using the Precise Four Terminal Network Method and Finite Element Method (FEM) and an ultrasonic generator to drive the probe is designed.The system was able to successfully puncture a single fish cell.

View Article: PubMed Central - PubMed

Affiliation: MEMS Lab, Department of Precision Instruments & Mechanology, Tsinghua University, Beijing 100084, P.R. China; E-Mails: zhouzy@mail.tsinghua.edu.cn ; yangxing@mail.tsinghua.edu.cn ; xiaomf03@mails.tsinghua.edu.cn.

ABSTRACT
Although ultrasound cavitation must be avoided for safe diagnostic applications, the ability of ultrasound to disrupt cell membranes has taken on increasing significance as a method to facilitate drug and gene delivery. A new ultrasonic resonance driving method is introduced to penetrate rigid wall plant cells or oocytes with springy cell membranes. When a reasonable design is created, ultrasound can gather energy and increase the amplitude factor. Ultrasonic penetration enables exogenous materials to enter cells without damaging them by utilizing instant acceleration. This paper seeks to develop a miniature ultrasonic probe experiment system for cell penetration. A miniature ultrasonic probe is designed and optimized using the Precise Four Terminal Network Method and Finite Element Method (FEM) and an ultrasonic generator to drive the probe is designed. The system was able to successfully puncture a single fish cell.

No MeSH data available.


Related in: MedlinePlus