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A miniature probe for ultrasonic penetration of a single cell.

Wu T, Zhou Z, Wang Q, Yang X, Xiao M - Sensors (Basel) (2009)

Bottom Line: This paper seeks to develop a miniature ultrasonic probe experiment system for cell penetration.A miniature ultrasonic probe is designed and optimized using the Precise Four Terminal Network Method and Finite Element Method (FEM) and an ultrasonic generator to drive the probe is designed.The system was able to successfully puncture a single fish cell.

View Article: PubMed Central - PubMed

Affiliation: MEMS Lab, Department of Precision Instruments & Mechanology, Tsinghua University, Beijing 100084, P.R. China; E-Mails: zhouzy@mail.tsinghua.edu.cn ; yangxing@mail.tsinghua.edu.cn ; xiaomf03@mails.tsinghua.edu.cn.

ABSTRACT
Although ultrasound cavitation must be avoided for safe diagnostic applications, the ability of ultrasound to disrupt cell membranes has taken on increasing significance as a method to facilitate drug and gene delivery. A new ultrasonic resonance driving method is introduced to penetrate rigid wall plant cells or oocytes with springy cell membranes. When a reasonable design is created, ultrasound can gather energy and increase the amplitude factor. Ultrasonic penetration enables exogenous materials to enter cells without damaging them by utilizing instant acceleration. This paper seeks to develop a miniature ultrasonic probe experiment system for cell penetration. A miniature ultrasonic probe is designed and optimized using the Precise Four Terminal Network Method and Finite Element Method (FEM) and an ultrasonic generator to drive the probe is designed. The system was able to successfully puncture a single fish cell.

No MeSH data available.


Related in: MedlinePlus

(a) Block diagram of ultrasonic generator. (b) A picture of ultrasonic generator.
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f2-sensors-09-03325: (a) Block diagram of ultrasonic generator. (b) A picture of ultrasonic generator.

Mentions: A series of experiments attempting to puncture a single cell were conducted, and they showed that when the ultrasonic probe was operated, its working characteristics would change. Compared with the unloaded condition the working frequency changed and the bandwidth of the admittance of the real part and radius of the admittance circle changed accordingly. In order to ensure that the probe is working in resonance and the output acoustic impedance matches the loading impedance, an automatic regulator had to be designed to regulate the frequency of the ultrasonic probe. A new ultrasonic generator, which consisted of a DDS synthesizer, a power amplifier and an automatic frequency regulator, was manufactured to improve the working stability of the system. Figure 2(a) presents the block diagram of its structure, and (b) is a picture of the ultrasonic generator be developed.


A miniature probe for ultrasonic penetration of a single cell.

Wu T, Zhou Z, Wang Q, Yang X, Xiao M - Sensors (Basel) (2009)

(a) Block diagram of ultrasonic generator. (b) A picture of ultrasonic generator.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3297146&req=5

f2-sensors-09-03325: (a) Block diagram of ultrasonic generator. (b) A picture of ultrasonic generator.
Mentions: A series of experiments attempting to puncture a single cell were conducted, and they showed that when the ultrasonic probe was operated, its working characteristics would change. Compared with the unloaded condition the working frequency changed and the bandwidth of the admittance of the real part and radius of the admittance circle changed accordingly. In order to ensure that the probe is working in resonance and the output acoustic impedance matches the loading impedance, an automatic regulator had to be designed to regulate the frequency of the ultrasonic probe. A new ultrasonic generator, which consisted of a DDS synthesizer, a power amplifier and an automatic frequency regulator, was manufactured to improve the working stability of the system. Figure 2(a) presents the block diagram of its structure, and (b) is a picture of the ultrasonic generator be developed.

Bottom Line: This paper seeks to develop a miniature ultrasonic probe experiment system for cell penetration.A miniature ultrasonic probe is designed and optimized using the Precise Four Terminal Network Method and Finite Element Method (FEM) and an ultrasonic generator to drive the probe is designed.The system was able to successfully puncture a single fish cell.

View Article: PubMed Central - PubMed

Affiliation: MEMS Lab, Department of Precision Instruments & Mechanology, Tsinghua University, Beijing 100084, P.R. China; E-Mails: zhouzy@mail.tsinghua.edu.cn ; yangxing@mail.tsinghua.edu.cn ; xiaomf03@mails.tsinghua.edu.cn.

ABSTRACT
Although ultrasound cavitation must be avoided for safe diagnostic applications, the ability of ultrasound to disrupt cell membranes has taken on increasing significance as a method to facilitate drug and gene delivery. A new ultrasonic resonance driving method is introduced to penetrate rigid wall plant cells or oocytes with springy cell membranes. When a reasonable design is created, ultrasound can gather energy and increase the amplitude factor. Ultrasonic penetration enables exogenous materials to enter cells without damaging them by utilizing instant acceleration. This paper seeks to develop a miniature ultrasonic probe experiment system for cell penetration. A miniature ultrasonic probe is designed and optimized using the Precise Four Terminal Network Method and Finite Element Method (FEM) and an ultrasonic generator to drive the probe is designed. The system was able to successfully puncture a single fish cell.

No MeSH data available.


Related in: MedlinePlus