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Feasibility studies on si-based biosensors.

Libertino S, Aiello V, Scandurra A, Renis M, Sinatra F, Lombardo S - Sensors (Basel) (2009)

Bottom Line: The importance of the added step is described by the experimental results.Finally a MOS-like structure was designed and fabricated to test an electrical transduction mechanism.The results obtained so far and the possible evolution of the research field are described in this review paper.

View Article: PubMed Central - PubMed

Affiliation: CNR - IMM Catania, Italy; E-Mail: salvatore.lombardo@imm.cnr.it.

ABSTRACT
The aim of this paper is to summarize the efforts carried out so far in the fabrication of Si-based biosensors by a team of researchers in Catania, Italy. This work was born as a collaboration between the Catania section of the Microelectronic and Microsystem Institute (IMM) of the CNR, the Surfaces and Interfaces laboratory (SUPERLAB) of the Consorzio Catania Ricerche and two departments at the University of Catania: the Biomedical Science and the Biological Chemistry and Molecular Biology Departments. The first goal of our study was the definition and optimization of an immobilization protocol capable of bonding the biological sensing element on a Si-based surface via covalent chemical bonds. We chose SiO(2) as the anchoring surface due to its biocompatibility and extensive presence in microelectronic devices. The immobilization protocol was tested and optimized, introducing a new step, oxide activation, using techniques compatible with microelectronic processing. The importance of the added step is described by the experimental results. We also tested different biological molecule concentrations in the immobilization solutions and the effects on the immobilized layer. Finally a MOS-like structure was designed and fabricated to test an electrical transduction mechanism. The results obtained so far and the possible evolution of the research field are described in this review paper.

No MeSH data available.


Capacitance voltage measurements carried out on reference sample. The first measure is reported in green cross. In the inset, the VFB extracted from the CV curves as a function of time (aging).
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f10-sensors-09-03469: Capacitance voltage measurements carried out on reference sample. The first measure is reported in green cross. In the inset, the VFB extracted from the CV curves as a function of time (aging).

Mentions: The MOS-like devices were prepared as described in the experimental section, in which all the fabrication details are reported, and the immobilization procedure already described was used. After immobilization, the samples were dried under a gentle Nitrogen flow and measured using capacitance-voltage (CV) measurements at 1 MHz, with a repetition frequency of 10 Hz and the curves were acquired during 100s of measurements. The results are shown in Figure 10 for the reference sample.


Feasibility studies on si-based biosensors.

Libertino S, Aiello V, Scandurra A, Renis M, Sinatra F, Lombardo S - Sensors (Basel) (2009)

Capacitance voltage measurements carried out on reference sample. The first measure is reported in green cross. In the inset, the VFB extracted from the CV curves as a function of time (aging).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3297125&req=5

f10-sensors-09-03469: Capacitance voltage measurements carried out on reference sample. The first measure is reported in green cross. In the inset, the VFB extracted from the CV curves as a function of time (aging).
Mentions: The MOS-like devices were prepared as described in the experimental section, in which all the fabrication details are reported, and the immobilization procedure already described was used. After immobilization, the samples were dried under a gentle Nitrogen flow and measured using capacitance-voltage (CV) measurements at 1 MHz, with a repetition frequency of 10 Hz and the curves were acquired during 100s of measurements. The results are shown in Figure 10 for the reference sample.

Bottom Line: The importance of the added step is described by the experimental results.Finally a MOS-like structure was designed and fabricated to test an electrical transduction mechanism.The results obtained so far and the possible evolution of the research field are described in this review paper.

View Article: PubMed Central - PubMed

Affiliation: CNR - IMM Catania, Italy; E-Mail: salvatore.lombardo@imm.cnr.it.

ABSTRACT
The aim of this paper is to summarize the efforts carried out so far in the fabrication of Si-based biosensors by a team of researchers in Catania, Italy. This work was born as a collaboration between the Catania section of the Microelectronic and Microsystem Institute (IMM) of the CNR, the Surfaces and Interfaces laboratory (SUPERLAB) of the Consorzio Catania Ricerche and two departments at the University of Catania: the Biomedical Science and the Biological Chemistry and Molecular Biology Departments. The first goal of our study was the definition and optimization of an immobilization protocol capable of bonding the biological sensing element on a Si-based surface via covalent chemical bonds. We chose SiO(2) as the anchoring surface due to its biocompatibility and extensive presence in microelectronic devices. The immobilization protocol was tested and optimized, introducing a new step, oxide activation, using techniques compatible with microelectronic processing. The importance of the added step is described by the experimental results. We also tested different biological molecule concentrations in the immobilization solutions and the effects on the immobilized layer. Finally a MOS-like structure was designed and fabricated to test an electrical transduction mechanism. The results obtained so far and the possible evolution of the research field are described in this review paper.

No MeSH data available.