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The jasmonate pathway mediates salt tolerance in grapevines.

Ismail A, Riemann M, Nick P - J. Exp. Bot. (2012)

Bottom Line: The rapid induction of RS and StSy characteristic for biotic defence in grapevine is strongly delayed in response to salt stress.In the salt-tolerant line, NHX1 is induced and the formation of reactive oxygen species, monitored as stress markers in the sensitive cell line, is suppressed.The data are discussed in terms of a model where salt stress signalling acts as a default pathway whose readout is modulated by a parallel signal chain triggered by biotic factors downstream of jasmonate signalling.

View Article: PubMed Central - PubMed

Affiliation: Karlsruhe Institute of Technology, Karlsruhe, Germany. ahmed.ismail@bio.uni-karlsruhe.de

ABSTRACT
Salt stress is a major constraint for many crop plants, such as the moderately salt-sensitive economically important fruit crop grapevine. Plants have evolved different strategies for protection against salinity and drought. Jasmonate signalling is a central element of both biotic and abiotic stress responses. To discriminate stress quality, there must be cross-talk with parallel signal chains. Using two grapevine cell lines differing in salt tolerance, the response of jasmonate ZIM/tify-domain (JAZ/TIFY) proteins (negative regulators of jasmonate signalling), a marker for salt adaptation Na(+)/H(+) EXCHANGER (NHX1), and markers for biotic defence STILBENE SYNTHASE (StSy) and RESVERATROL SYNTHASE (RS) were analysed. It is shown that salt stress signalling shares several events with biotic defence including activity of a gadolinium-sensitive calcium influx channel (monitored by apoplastic alkalinization) and transient induction of JAZ/TIFY transcripts. Exogenous jasmonate can rescue growth in the salt-sensitive cell line. Suppression of jasmonate signalling by phenidone or aspirin blocks the induction of JAZ/TIFY transcripts. The rapid induction of RS and StSy characteristic for biotic defence in grapevine is strongly delayed in response to salt stress. In the salt-tolerant line, NHX1 is induced and the formation of reactive oxygen species, monitored as stress markers in the sensitive cell line, is suppressed. The data are discussed in terms of a model where salt stress signalling acts as a default pathway whose readout is modulated by a parallel signal chain triggered by biotic factors downstream of jasmonate signalling.

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Effect of Harpin elicitor on the induction of JAZ1/TIFY10a and MYC2 transcripts in V. rupestris (Rup). Cells were treated with 9 μg ml−1 active elicitor for 1 h. The transcript abundance of JAZ1/TIFY10a and MYC2 is given relative to elongation factor 1α (EF1-α). The data represent averages of three independent experimental series; error bars represent standard errors.
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fig8: Effect of Harpin elicitor on the induction of JAZ1/TIFY10a and MYC2 transcripts in V. rupestris (Rup). Cells were treated with 9 μg ml−1 active elicitor for 1 h. The transcript abundance of JAZ1/TIFY10a and MYC2 is given relative to elongation factor 1α (EF1-α). The data represent averages of three independent experimental series; error bars represent standard errors.

Mentions: Since JA has been shown to trigger phytoalexin synthesis in Vitis (Tassoni et al., 2005), a typical biotic stress response, and since salt stress was found to induce RS and StSy in V. rupestris, tests were carried out to determine whether JAZ1/TIFY10a can be induced in the absence of salt by triggering biotic defence. For this purpose the elicitor Harpin that, in V. rupestris, can induce the entire programme of defence (Qiao et al., 2010), was used. In fact, a clear induction of JAZ1/TIFY10a (Fig. 8) could be observed to a level similar to that observed after treatment with NaCl (Fig. 5; Supplementary Table S3 at JXB online). Also MYC2 showed a slight induction, similar to that observed after salt treatment (Fig. 5; Supplementary Table S3). Thus, the primary events of biotic and salt signalling including extracellular alkalinization, induction of JAZ1/TIFY10a, and (slight) induction of MYC2, seem to be very similar.


The jasmonate pathway mediates salt tolerance in grapevines.

Ismail A, Riemann M, Nick P - J. Exp. Bot. (2012)

Effect of Harpin elicitor on the induction of JAZ1/TIFY10a and MYC2 transcripts in V. rupestris (Rup). Cells were treated with 9 μg ml−1 active elicitor for 1 h. The transcript abundance of JAZ1/TIFY10a and MYC2 is given relative to elongation factor 1α (EF1-α). The data represent averages of three independent experimental series; error bars represent standard errors.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3295401&req=5

fig8: Effect of Harpin elicitor on the induction of JAZ1/TIFY10a and MYC2 transcripts in V. rupestris (Rup). Cells were treated with 9 μg ml−1 active elicitor for 1 h. The transcript abundance of JAZ1/TIFY10a and MYC2 is given relative to elongation factor 1α (EF1-α). The data represent averages of three independent experimental series; error bars represent standard errors.
Mentions: Since JA has been shown to trigger phytoalexin synthesis in Vitis (Tassoni et al., 2005), a typical biotic stress response, and since salt stress was found to induce RS and StSy in V. rupestris, tests were carried out to determine whether JAZ1/TIFY10a can be induced in the absence of salt by triggering biotic defence. For this purpose the elicitor Harpin that, in V. rupestris, can induce the entire programme of defence (Qiao et al., 2010), was used. In fact, a clear induction of JAZ1/TIFY10a (Fig. 8) could be observed to a level similar to that observed after treatment with NaCl (Fig. 5; Supplementary Table S3 at JXB online). Also MYC2 showed a slight induction, similar to that observed after salt treatment (Fig. 5; Supplementary Table S3). Thus, the primary events of biotic and salt signalling including extracellular alkalinization, induction of JAZ1/TIFY10a, and (slight) induction of MYC2, seem to be very similar.

Bottom Line: The rapid induction of RS and StSy characteristic for biotic defence in grapevine is strongly delayed in response to salt stress.In the salt-tolerant line, NHX1 is induced and the formation of reactive oxygen species, monitored as stress markers in the sensitive cell line, is suppressed.The data are discussed in terms of a model where salt stress signalling acts as a default pathway whose readout is modulated by a parallel signal chain triggered by biotic factors downstream of jasmonate signalling.

View Article: PubMed Central - PubMed

Affiliation: Karlsruhe Institute of Technology, Karlsruhe, Germany. ahmed.ismail@bio.uni-karlsruhe.de

ABSTRACT
Salt stress is a major constraint for many crop plants, such as the moderately salt-sensitive economically important fruit crop grapevine. Plants have evolved different strategies for protection against salinity and drought. Jasmonate signalling is a central element of both biotic and abiotic stress responses. To discriminate stress quality, there must be cross-talk with parallel signal chains. Using two grapevine cell lines differing in salt tolerance, the response of jasmonate ZIM/tify-domain (JAZ/TIFY) proteins (negative regulators of jasmonate signalling), a marker for salt adaptation Na(+)/H(+) EXCHANGER (NHX1), and markers for biotic defence STILBENE SYNTHASE (StSy) and RESVERATROL SYNTHASE (RS) were analysed. It is shown that salt stress signalling shares several events with biotic defence including activity of a gadolinium-sensitive calcium influx channel (monitored by apoplastic alkalinization) and transient induction of JAZ/TIFY transcripts. Exogenous jasmonate can rescue growth in the salt-sensitive cell line. Suppression of jasmonate signalling by phenidone or aspirin blocks the induction of JAZ/TIFY transcripts. The rapid induction of RS and StSy characteristic for biotic defence in grapevine is strongly delayed in response to salt stress. In the salt-tolerant line, NHX1 is induced and the formation of reactive oxygen species, monitored as stress markers in the sensitive cell line, is suppressed. The data are discussed in terms of a model where salt stress signalling acts as a default pathway whose readout is modulated by a parallel signal chain triggered by biotic factors downstream of jasmonate signalling.

Show MeSH
Related in: MedlinePlus