Limits...
BnMs3 is required for tapetal differentiation and degradation, microspore separation, and pollen-wall biosynthesis in Brassica napus.

Zhou Z, Dun X, Xia S, Shi D, Qin M, Yi B, Wen J, Shen J, Ma C, Tu J, Fu T - J. Exp. Bot. (2011)

Bottom Line: A total of 76 down-regulated unigenes in the Bnms3 mutant, several of which are associated with tapetum development, callose degeneration, and pollen development, were isolated by suppression subtractive hybridization combined with a macroarray analysis.Moreover, BnMs3 takes part in pollen-wall formation by affecting the expression of a series of genes involved in biosynthesis and transport of sporopollenin precursors.All of the above results suggest that BnMs3 participates in tapetum development, microspore release, and pollen-wall formation in B. napus.

View Article: PubMed Central - PubMed

Affiliation: Huazhong Agricultural University, Wuhan, China.

ABSTRACT
7365AB, a recessive genetic male sterility system, is controlled by BnMs3 in Brassica napus, which encodes a Tic40 protein required for tapetum development. However, the role of BnMs3 in rapeseed anther development is still largely unclear. In this research, cytological analysis revealed that anther development of a Bnms3 mutant has defects in the transition of the tapetum to the secretory type, callose degradation, and pollen-wall formation. A total of 76 down-regulated unigenes in the Bnms3 mutant, several of which are associated with tapetum development, callose degeneration, and pollen development, were isolated by suppression subtractive hybridization combined with a macroarray analysis. Reverse genetics was applied by means of Arabidopsis insertional mutant lines to characterize the function of these unigenes and revealed that MSR02 is only required for transport of sporopollenin precursors through the plasma membrane of the tapetum. The real-time PCR data have further verified that BnMs3 plays a primary role in tapetal differentiation by affecting the expression of a few key transcription factors, participates in tapetal degradation by modulating the expression of cysteine protease genes, and influences microspore separation by manipulating the expression of BnA6 and BnMSR66 related to callose degradation and of BnQRT1 and BnQRT3 required for the primary cell-wall degradation of the pollen mother cell. Moreover, BnMs3 takes part in pollen-wall formation by affecting the expression of a series of genes involved in biosynthesis and transport of sporopollenin precursors. All of the above results suggest that BnMs3 participates in tapetum development, microspore release, and pollen-wall formation in B. napus.

Show MeSH

Related in: MedlinePlus

Functional categorization of down-regulated unigenes contained in the Bnms3 mutant according to the Munich Information Center for Protein Sequences classification. A total of 68 unigenes were grouped into eight functional categories and the percentages of gene transcripts in each group were listed. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC3295392&req=5

fig3: Functional categorization of down-regulated unigenes contained in the Bnms3 mutant according to the Munich Information Center for Protein Sequences classification. A total of 68 unigenes were grouped into eight functional categories and the percentages of gene transcripts in each group were listed. (This figure is available in colour at JXB online.)

Mentions: To gain further insights into the functions of the differentially expressed genes, the 68 annotated down-regulated unigenes in the Bnms3 mutant were classified into eight functional categories based on the information from MIPS (Fig. 3; Supplementary Table S1). The major functional groups of down-regulated unigenes were concerned with metabolism, lipid/fatty acid transport, and protein synthesis. The first main group comprised 20 unigenes related to metabolism, encoding enzymes such as cytochrome P450 monooxygenase, glyceraldehyde-3-phosphate dehydrogenase, fatty acid elongase 3-ketoacyl-CoA synthase 7, and caffeoyl-CoA 3-O-methyltransferase. The second main group included nine unigenes annotated as one ABC transport protein and eight LTPs involved in lipid/fatty acid transport. Moreover, six unigenes were associated with protein synthesis, such as translation elongation factor 2-like proteins and ribosomal proteins. The other unigenes were related to transport, protein fate, and stress-responsive proteins.


BnMs3 is required for tapetal differentiation and degradation, microspore separation, and pollen-wall biosynthesis in Brassica napus.

Zhou Z, Dun X, Xia S, Shi D, Qin M, Yi B, Wen J, Shen J, Ma C, Tu J, Fu T - J. Exp. Bot. (2011)

Functional categorization of down-regulated unigenes contained in the Bnms3 mutant according to the Munich Information Center for Protein Sequences classification. A total of 68 unigenes were grouped into eight functional categories and the percentages of gene transcripts in each group were listed. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3295392&req=5

fig3: Functional categorization of down-regulated unigenes contained in the Bnms3 mutant according to the Munich Information Center for Protein Sequences classification. A total of 68 unigenes were grouped into eight functional categories and the percentages of gene transcripts in each group were listed. (This figure is available in colour at JXB online.)
Mentions: To gain further insights into the functions of the differentially expressed genes, the 68 annotated down-regulated unigenes in the Bnms3 mutant were classified into eight functional categories based on the information from MIPS (Fig. 3; Supplementary Table S1). The major functional groups of down-regulated unigenes were concerned with metabolism, lipid/fatty acid transport, and protein synthesis. The first main group comprised 20 unigenes related to metabolism, encoding enzymes such as cytochrome P450 monooxygenase, glyceraldehyde-3-phosphate dehydrogenase, fatty acid elongase 3-ketoacyl-CoA synthase 7, and caffeoyl-CoA 3-O-methyltransferase. The second main group included nine unigenes annotated as one ABC transport protein and eight LTPs involved in lipid/fatty acid transport. Moreover, six unigenes were associated with protein synthesis, such as translation elongation factor 2-like proteins and ribosomal proteins. The other unigenes were related to transport, protein fate, and stress-responsive proteins.

Bottom Line: A total of 76 down-regulated unigenes in the Bnms3 mutant, several of which are associated with tapetum development, callose degeneration, and pollen development, were isolated by suppression subtractive hybridization combined with a macroarray analysis.Moreover, BnMs3 takes part in pollen-wall formation by affecting the expression of a series of genes involved in biosynthesis and transport of sporopollenin precursors.All of the above results suggest that BnMs3 participates in tapetum development, microspore release, and pollen-wall formation in B. napus.

View Article: PubMed Central - PubMed

Affiliation: Huazhong Agricultural University, Wuhan, China.

ABSTRACT
7365AB, a recessive genetic male sterility system, is controlled by BnMs3 in Brassica napus, which encodes a Tic40 protein required for tapetum development. However, the role of BnMs3 in rapeseed anther development is still largely unclear. In this research, cytological analysis revealed that anther development of a Bnms3 mutant has defects in the transition of the tapetum to the secretory type, callose degradation, and pollen-wall formation. A total of 76 down-regulated unigenes in the Bnms3 mutant, several of which are associated with tapetum development, callose degeneration, and pollen development, were isolated by suppression subtractive hybridization combined with a macroarray analysis. Reverse genetics was applied by means of Arabidopsis insertional mutant lines to characterize the function of these unigenes and revealed that MSR02 is only required for transport of sporopollenin precursors through the plasma membrane of the tapetum. The real-time PCR data have further verified that BnMs3 plays a primary role in tapetal differentiation by affecting the expression of a few key transcription factors, participates in tapetal degradation by modulating the expression of cysteine protease genes, and influences microspore separation by manipulating the expression of BnA6 and BnMSR66 related to callose degradation and of BnQRT1 and BnQRT3 required for the primary cell-wall degradation of the pollen mother cell. Moreover, BnMs3 takes part in pollen-wall formation by affecting the expression of a series of genes involved in biosynthesis and transport of sporopollenin precursors. All of the above results suggest that BnMs3 participates in tapetum development, microspore release, and pollen-wall formation in B. napus.

Show MeSH
Related in: MedlinePlus