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Arabidopsis thaliana DOF6 negatively affects germination in non-after-ripened seeds and interacts with TCP14.

Rueda-Romero P, Barrero-Sicilia C, Gómez-Cadenas A, Carbonero P, Oñate-Sánchez L - J. Exp. Bot. (2011)

Bottom Line: While constitutive over-expression of DOF6 produced aberrant growth and sterility in the plant, its over-expression induced upon seed imbibition triggered delayed germination, abscisic acid (ABA)-hypersensitive phenotypes and increased expression of the ABA biosynthetic gene ABA1 and ABA-related stress genes.DOF6 was found to interact in a yeast two-hybrid system and in planta with TCP14, a previously described positive regulator of seed germination.The expression of ABA1 and ABA-related stress genes was also enhanced in tcp14 knock-out mutants.

View Article: PubMed Central - PubMed

Affiliation: Universidad Politécnica de Madrid, Campus de Montegancedo, Pozuelo de Alarcón, Madrid, Spain.

ABSTRACT
Seed dormancy prevents seeds from germinating under environmental conditions unfavourable for plant growth and development and constitutes an evolutionary advantage. Dry storage, also known as after-ripening, gradually decreases seed dormancy by mechanisms not well understood. An Arabidopsis thaliana DOF transcription factor gene (DOF6) affecting seed germination has been characterized. The transcript levels of this gene accumulate in dry seeds and decay gradually during after-ripening and also upon seed imbibition. While constitutive over-expression of DOF6 produced aberrant growth and sterility in the plant, its over-expression induced upon seed imbibition triggered delayed germination, abscisic acid (ABA)-hypersensitive phenotypes and increased expression of the ABA biosynthetic gene ABA1 and ABA-related stress genes. Wild-type germination and gene expression were gradually restored during seed after-ripening, despite of DOF6-induced over-expression. DOF6 was found to interact in a yeast two-hybrid system and in planta with TCP14, a previously described positive regulator of seed germination. The expression of ABA1 and ABA-related stress genes was also enhanced in tcp14 knock-out mutants. Taken together, these results indicate that DOF6 negatively affects seed germination and opposes TCP14 function in the regulation of a specific set of ABA-related genes.

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Related in: MedlinePlus

ABA effect on DOF6 expression and ioexDOF6 seed germination. (A and B) DOF6 expression by quantitative RT-PCR in freshly harvested (FH; A) and after-ripened (AR; B) Col-0 seeds imbibed in water with 2 and 5 μM ABA, respectively. (C and D) Germination of FH (C) and AR (D) wild-type (WT) and ioexDOF6 seeds in water supplemented with 50 μM estradiol and 0.5 and 1 μM ABA, respectively. Germinating percentages are represented as the mean±standard error from three replicates. Germination patterns were confirmed in two different seed batches. Gene-specific mRNA levels are relative to UBC21.
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fig4: ABA effect on DOF6 expression and ioexDOF6 seed germination. (A and B) DOF6 expression by quantitative RT-PCR in freshly harvested (FH; A) and after-ripened (AR; B) Col-0 seeds imbibed in water with 2 and 5 μM ABA, respectively. (C and D) Germination of FH (C) and AR (D) wild-type (WT) and ioexDOF6 seeds in water supplemented with 50 μM estradiol and 0.5 and 1 μM ABA, respectively. Germinating percentages are represented as the mean±standard error from three replicates. Germination patterns were confirmed in two different seed batches. Gene-specific mRNA levels are relative to UBC21.

Mentions: Since DOF6 mRNA levels in seeds decreased during after-ripening and upon seed imbibition and increased during seed maturation, a pattern that resembled ABA dynamics in seeds (Kushiro et al., 2004; Vicente-Carbajosa and Carbonero, 2005; Millar et al., 2006), it seemed appropriate to quantify DOF6 mRNA levels in seeds in response to ABA. FH and AR WT seeds were imbibed in the presence or absence of ABA, and DOF6 transcripts were quantified during seed imbibition. As shown in Fig. 4A and B, DOF6 had different expression patterns in FH and AR imbibed seeds, both with or without ABA during the first hours of imbibition, but the transcript levels were not significantly altered by the presence of ABA after 24 h.


Arabidopsis thaliana DOF6 negatively affects germination in non-after-ripened seeds and interacts with TCP14.

Rueda-Romero P, Barrero-Sicilia C, Gómez-Cadenas A, Carbonero P, Oñate-Sánchez L - J. Exp. Bot. (2011)

ABA effect on DOF6 expression and ioexDOF6 seed germination. (A and B) DOF6 expression by quantitative RT-PCR in freshly harvested (FH; A) and after-ripened (AR; B) Col-0 seeds imbibed in water with 2 and 5 μM ABA, respectively. (C and D) Germination of FH (C) and AR (D) wild-type (WT) and ioexDOF6 seeds in water supplemented with 50 μM estradiol and 0.5 and 1 μM ABA, respectively. Germinating percentages are represented as the mean±standard error from three replicates. Germination patterns were confirmed in two different seed batches. Gene-specific mRNA levels are relative to UBC21.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3295388&req=5

fig4: ABA effect on DOF6 expression and ioexDOF6 seed germination. (A and B) DOF6 expression by quantitative RT-PCR in freshly harvested (FH; A) and after-ripened (AR; B) Col-0 seeds imbibed in water with 2 and 5 μM ABA, respectively. (C and D) Germination of FH (C) and AR (D) wild-type (WT) and ioexDOF6 seeds in water supplemented with 50 μM estradiol and 0.5 and 1 μM ABA, respectively. Germinating percentages are represented as the mean±standard error from three replicates. Germination patterns were confirmed in two different seed batches. Gene-specific mRNA levels are relative to UBC21.
Mentions: Since DOF6 mRNA levels in seeds decreased during after-ripening and upon seed imbibition and increased during seed maturation, a pattern that resembled ABA dynamics in seeds (Kushiro et al., 2004; Vicente-Carbajosa and Carbonero, 2005; Millar et al., 2006), it seemed appropriate to quantify DOF6 mRNA levels in seeds in response to ABA. FH and AR WT seeds were imbibed in the presence or absence of ABA, and DOF6 transcripts were quantified during seed imbibition. As shown in Fig. 4A and B, DOF6 had different expression patterns in FH and AR imbibed seeds, both with or without ABA during the first hours of imbibition, but the transcript levels were not significantly altered by the presence of ABA after 24 h.

Bottom Line: While constitutive over-expression of DOF6 produced aberrant growth and sterility in the plant, its over-expression induced upon seed imbibition triggered delayed germination, abscisic acid (ABA)-hypersensitive phenotypes and increased expression of the ABA biosynthetic gene ABA1 and ABA-related stress genes.DOF6 was found to interact in a yeast two-hybrid system and in planta with TCP14, a previously described positive regulator of seed germination.The expression of ABA1 and ABA-related stress genes was also enhanced in tcp14 knock-out mutants.

View Article: PubMed Central - PubMed

Affiliation: Universidad Politécnica de Madrid, Campus de Montegancedo, Pozuelo de Alarcón, Madrid, Spain.

ABSTRACT
Seed dormancy prevents seeds from germinating under environmental conditions unfavourable for plant growth and development and constitutes an evolutionary advantage. Dry storage, also known as after-ripening, gradually decreases seed dormancy by mechanisms not well understood. An Arabidopsis thaliana DOF transcription factor gene (DOF6) affecting seed germination has been characterized. The transcript levels of this gene accumulate in dry seeds and decay gradually during after-ripening and also upon seed imbibition. While constitutive over-expression of DOF6 produced aberrant growth and sterility in the plant, its over-expression induced upon seed imbibition triggered delayed germination, abscisic acid (ABA)-hypersensitive phenotypes and increased expression of the ABA biosynthetic gene ABA1 and ABA-related stress genes. Wild-type germination and gene expression were gradually restored during seed after-ripening, despite of DOF6-induced over-expression. DOF6 was found to interact in a yeast two-hybrid system and in planta with TCP14, a previously described positive regulator of seed germination. The expression of ABA1 and ABA-related stress genes was also enhanced in tcp14 knock-out mutants. Taken together, these results indicate that DOF6 negatively affects seed germination and opposes TCP14 function in the regulation of a specific set of ABA-related genes.

Show MeSH
Related in: MedlinePlus