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Overt and latent cardiac effects of ozone inhalation in rats: evidence for autonomic modulation and increased myocardial vulnerability.

Farraj AK, Hazari MS, Winsett DW, Kulukulualani A, Carll AP, Haykal-Coates N, Lamb CM, Lappi E, Terrell D, Cascio WE, Costa DL - Environ. Health Perspect. (2011)

Bottom Line: Ozone (O₃) is a well-documented respiratory oxidant, but increasing epidemiological evidence points to extrapulmonary effects, including positive associations between ambient O₃ concentrations and cardiovascular morbidity and mortality.However, both 0.2 and 0.8 ppm O₃ increased sensitivity to aconitine-induced arrhythmia formation, suggesting a latent O₃-induced alteration in myocardial excitability.O₃ exposure causes several alterations in cardiac electrophysiology that are likely mediated by modulation of autonomic input to the heart.

View Article: PubMed Central - PubMed

Affiliation: Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA. farraj.aimen@epa.gov

ABSTRACT

Background: Ozone (O₃) is a well-documented respiratory oxidant, but increasing epidemiological evidence points to extrapulmonary effects, including positive associations between ambient O₃ concentrations and cardiovascular morbidity and mortality.

Objective: With preliminary reports linking O₃ exposure with changes in heart rate (HR), we investigated the hypothesis that a single inhalation exposure to O₃ will cause concentration-dependent autonomic modulation of cardiac function in rats.

Methods: Rats implanted with telemeters to monitor HR and cardiac electrophysiology [electrocardiography (ECG)] were exposed once by whole-body inhalation for 4 hr to 0.2 or 0.8 ppm O₃ or filtered air. A separate cohort was tested for vulnerability to aconitine-induced arrhythmia 24 hr after exposure.

Results: Exposure to 0.8 ppm O₃ caused bradycardia, PR prolongation, ST depression, and substantial increases in atrial premature beats, sinoatrial block, and atrioventricular block, accompanied by concurrent increases in several HR variability parameters that were suggestive of increased parasympathetic tone. Low-O₃ exposure failed to elicit any overt changes in autonomic tone, heart rhythm, or ECG. However, both 0.2 and 0.8 ppm O₃ increased sensitivity to aconitine-induced arrhythmia formation, suggesting a latent O₃-induced alteration in myocardial excitability.

Conclusions: O₃ exposure causes several alterations in cardiac electrophysiology that are likely mediated by modulation of autonomic input to the heart. Moreover, exposure to low O₃ concentrations may cause subclinical effects that manifest only when triggered by a stressor, suggesting that the adverse health effects of ambient levels of air pollutants may be insidious and potentially underestimated.

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Related in: MedlinePlus

HR (A), PR interval (B), QTc (C), and ST area (D) immediately before (baseline) and during (exposure) 4-hr exposure to 0.2 ppm or 0.8 ppm O3 or filtered air (mean ± SE, n = 6). *Significantly less than preexposure baseline values within corresponding group (p < 0.05). **Significantly greater than preexposure baseline values within corresponding group (p < 0.05).
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f1: HR (A), PR interval (B), QTc (C), and ST area (D) immediately before (baseline) and during (exposure) 4-hr exposure to 0.2 ppm or 0.8 ppm O3 or filtered air (mean ± SE, n = 6). *Significantly less than preexposure baseline values within corresponding group (p < 0.05). **Significantly greater than preexposure baseline values within corresponding group (p < 0.05).

Mentions: HR and ECG morphology. High-O3 exposure caused a significant decrease in HR (22.1%; p < 0.05) relative to preexposure baseline values (Figure 1). There was no significant effect of low-O3 or air exposure on HR.


Overt and latent cardiac effects of ozone inhalation in rats: evidence for autonomic modulation and increased myocardial vulnerability.

Farraj AK, Hazari MS, Winsett DW, Kulukulualani A, Carll AP, Haykal-Coates N, Lamb CM, Lappi E, Terrell D, Cascio WE, Costa DL - Environ. Health Perspect. (2011)

HR (A), PR interval (B), QTc (C), and ST area (D) immediately before (baseline) and during (exposure) 4-hr exposure to 0.2 ppm or 0.8 ppm O3 or filtered air (mean ± SE, n = 6). *Significantly less than preexposure baseline values within corresponding group (p < 0.05). **Significantly greater than preexposure baseline values within corresponding group (p < 0.05).
© Copyright Policy - public-domain
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3295357&req=5

f1: HR (A), PR interval (B), QTc (C), and ST area (D) immediately before (baseline) and during (exposure) 4-hr exposure to 0.2 ppm or 0.8 ppm O3 or filtered air (mean ± SE, n = 6). *Significantly less than preexposure baseline values within corresponding group (p < 0.05). **Significantly greater than preexposure baseline values within corresponding group (p < 0.05).
Mentions: HR and ECG morphology. High-O3 exposure caused a significant decrease in HR (22.1%; p < 0.05) relative to preexposure baseline values (Figure 1). There was no significant effect of low-O3 or air exposure on HR.

Bottom Line: Ozone (O₃) is a well-documented respiratory oxidant, but increasing epidemiological evidence points to extrapulmonary effects, including positive associations between ambient O₃ concentrations and cardiovascular morbidity and mortality.However, both 0.2 and 0.8 ppm O₃ increased sensitivity to aconitine-induced arrhythmia formation, suggesting a latent O₃-induced alteration in myocardial excitability.O₃ exposure causes several alterations in cardiac electrophysiology that are likely mediated by modulation of autonomic input to the heart.

View Article: PubMed Central - PubMed

Affiliation: Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA. farraj.aimen@epa.gov

ABSTRACT

Background: Ozone (O₃) is a well-documented respiratory oxidant, but increasing epidemiological evidence points to extrapulmonary effects, including positive associations between ambient O₃ concentrations and cardiovascular morbidity and mortality.

Objective: With preliminary reports linking O₃ exposure with changes in heart rate (HR), we investigated the hypothesis that a single inhalation exposure to O₃ will cause concentration-dependent autonomic modulation of cardiac function in rats.

Methods: Rats implanted with telemeters to monitor HR and cardiac electrophysiology [electrocardiography (ECG)] were exposed once by whole-body inhalation for 4 hr to 0.2 or 0.8 ppm O₃ or filtered air. A separate cohort was tested for vulnerability to aconitine-induced arrhythmia 24 hr after exposure.

Results: Exposure to 0.8 ppm O₃ caused bradycardia, PR prolongation, ST depression, and substantial increases in atrial premature beats, sinoatrial block, and atrioventricular block, accompanied by concurrent increases in several HR variability parameters that were suggestive of increased parasympathetic tone. Low-O₃ exposure failed to elicit any overt changes in autonomic tone, heart rhythm, or ECG. However, both 0.2 and 0.8 ppm O₃ increased sensitivity to aconitine-induced arrhythmia formation, suggesting a latent O₃-induced alteration in myocardial excitability.

Conclusions: O₃ exposure causes several alterations in cardiac electrophysiology that are likely mediated by modulation of autonomic input to the heart. Moreover, exposure to low O₃ concentrations may cause subclinical effects that manifest only when triggered by a stressor, suggesting that the adverse health effects of ambient levels of air pollutants may be insidious and potentially underestimated.

Show MeSH
Related in: MedlinePlus