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Analysis of avian hepatitis E virus from chickens, China.

Zhao Q, Zhou EM, Dong SW, Qiu HK, Zhang L, Hu SB, Zhao FF, Jiang SJ, Sun YN - Emerging Infect. Dis. (2010)

Bottom Line: Avian hepatitis E virus (HEV) has been identified in chickens; however, only 4 complete or near-complete genomic sequences have been reported.We found that the near-complete genomic sequence of avian HEV in chickens from China shared the highest identity (98.3%) with avian HEV from Europe and belonged to avian HEV genotype 3.

View Article: PubMed Central - PubMed

Affiliation: Shandong Agricultural University, Shandong, People's Republic of China.

ABSTRACT
Avian hepatitis E virus (HEV) has been identified in chickens; however, only 4 complete or near-complete genomic sequences have been reported. We found that the near-complete genomic sequence of avian HEV in chickens from China shared the highest identity (98.3%) with avian HEV from Europe and belonged to avian HEV genotype 3.

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Amino acid sequence comparison of motif VII in the open reading frame (ORF) 1 RNA-dependent RNA polymerase (RdRp) region of avian, human, and swine hepatitis E viruses (HEVs) (A), antigenic domain II (B), and antigenic domain IV (C) in the ORF2 region of avian HEV. Residues that are conserved among avian HEV (aHEV) isolates are shown as the consensus above the sequences; residues that are conserved in the HEV strains are not shown. GenBank accession numbers of human and swine HEV (sHEV) strains are M73218 (Burma), AF076239 (Hyderabad), D11092 (China Xingjiang), M74506 (Mexico), AF082843 (prototype sHEV), FJ527832 (China Shanghai), AB291955 (Japan Shinagawa), AJ272108 (China T1), AB480825 (Japan HE-JF5), and FJ763142 (Korea). GenBank accession numbers of avian HEV strains are EF206691 (“avirulent aHEV” from the United States), AM535004 (prototype aHEV from the United States), AM943647 (aHEV from Australia [AaHEV]), AM943646 (aHEV from Europe [EaHEV]), and GU954430 (aHEV from China [CaHEV]). Boxes indicate mutations of CaHEV compared with different HEV strains.
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Figure 1: Amino acid sequence comparison of motif VII in the open reading frame (ORF) 1 RNA-dependent RNA polymerase (RdRp) region of avian, human, and swine hepatitis E viruses (HEVs) (A), antigenic domain II (B), and antigenic domain IV (C) in the ORF2 region of avian HEV. Residues that are conserved among avian HEV (aHEV) isolates are shown as the consensus above the sequences; residues that are conserved in the HEV strains are not shown. GenBank accession numbers of human and swine HEV (sHEV) strains are M73218 (Burma), AF076239 (Hyderabad), D11092 (China Xingjiang), M74506 (Mexico), AF082843 (prototype sHEV), FJ527832 (China Shanghai), AB291955 (Japan Shinagawa), AJ272108 (China T1), AB480825 (Japan HE-JF5), and FJ763142 (Korea). GenBank accession numbers of avian HEV strains are EF206691 (“avirulent aHEV” from the United States), AM535004 (prototype aHEV from the United States), AM943647 (aHEV from Australia [AaHEV]), AM943646 (aHEV from Europe [EaHEV]), and GU954430 (aHEV from China [CaHEV]). Boxes indicate mutations of CaHEV compared with different HEV strains.

Mentions: ORF1 of CaHEV contained most mutations compared with prototype avian HEV (prototype aHEV); 5, 16, and 29 nonsilent mutations occurred in the methyltransferase, helicase, and RNA-dependent RNA polymerase (RdRp) functional domains, respectively (data not shown). However, only 2 mutations occurred in motif VII of RdRp domain (Figure 1, panel A), which contains 8 motifs responsible for virus replication (13). The 2 mutations in motif VII of the CaHEV RdRp domain are L(1432)M and I(1434)V. Australian avian HEV isolate (AaHEV) also has the mutation in the latter position and was a transition from I(1433) to T (Figure 1, panel A). This position is well conserved among mammalian HEV isolates by the presence of V, which is the same as CaHEV (Figure 1, panel A).


Analysis of avian hepatitis E virus from chickens, China.

Zhao Q, Zhou EM, Dong SW, Qiu HK, Zhang L, Hu SB, Zhao FF, Jiang SJ, Sun YN - Emerging Infect. Dis. (2010)

Amino acid sequence comparison of motif VII in the open reading frame (ORF) 1 RNA-dependent RNA polymerase (RdRp) region of avian, human, and swine hepatitis E viruses (HEVs) (A), antigenic domain II (B), and antigenic domain IV (C) in the ORF2 region of avian HEV. Residues that are conserved among avian HEV (aHEV) isolates are shown as the consensus above the sequences; residues that are conserved in the HEV strains are not shown. GenBank accession numbers of human and swine HEV (sHEV) strains are M73218 (Burma), AF076239 (Hyderabad), D11092 (China Xingjiang), M74506 (Mexico), AF082843 (prototype sHEV), FJ527832 (China Shanghai), AB291955 (Japan Shinagawa), AJ272108 (China T1), AB480825 (Japan HE-JF5), and FJ763142 (Korea). GenBank accession numbers of avian HEV strains are EF206691 (“avirulent aHEV” from the United States), AM535004 (prototype aHEV from the United States), AM943647 (aHEV from Australia [AaHEV]), AM943646 (aHEV from Europe [EaHEV]), and GU954430 (aHEV from China [CaHEV]). Boxes indicate mutations of CaHEV compared with different HEV strains.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3294992&req=5

Figure 1: Amino acid sequence comparison of motif VII in the open reading frame (ORF) 1 RNA-dependent RNA polymerase (RdRp) region of avian, human, and swine hepatitis E viruses (HEVs) (A), antigenic domain II (B), and antigenic domain IV (C) in the ORF2 region of avian HEV. Residues that are conserved among avian HEV (aHEV) isolates are shown as the consensus above the sequences; residues that are conserved in the HEV strains are not shown. GenBank accession numbers of human and swine HEV (sHEV) strains are M73218 (Burma), AF076239 (Hyderabad), D11092 (China Xingjiang), M74506 (Mexico), AF082843 (prototype sHEV), FJ527832 (China Shanghai), AB291955 (Japan Shinagawa), AJ272108 (China T1), AB480825 (Japan HE-JF5), and FJ763142 (Korea). GenBank accession numbers of avian HEV strains are EF206691 (“avirulent aHEV” from the United States), AM535004 (prototype aHEV from the United States), AM943647 (aHEV from Australia [AaHEV]), AM943646 (aHEV from Europe [EaHEV]), and GU954430 (aHEV from China [CaHEV]). Boxes indicate mutations of CaHEV compared with different HEV strains.
Mentions: ORF1 of CaHEV contained most mutations compared with prototype avian HEV (prototype aHEV); 5, 16, and 29 nonsilent mutations occurred in the methyltransferase, helicase, and RNA-dependent RNA polymerase (RdRp) functional domains, respectively (data not shown). However, only 2 mutations occurred in motif VII of RdRp domain (Figure 1, panel A), which contains 8 motifs responsible for virus replication (13). The 2 mutations in motif VII of the CaHEV RdRp domain are L(1432)M and I(1434)V. Australian avian HEV isolate (AaHEV) also has the mutation in the latter position and was a transition from I(1433) to T (Figure 1, panel A). This position is well conserved among mammalian HEV isolates by the presence of V, which is the same as CaHEV (Figure 1, panel A).

Bottom Line: Avian hepatitis E virus (HEV) has been identified in chickens; however, only 4 complete or near-complete genomic sequences have been reported.We found that the near-complete genomic sequence of avian HEV in chickens from China shared the highest identity (98.3%) with avian HEV from Europe and belonged to avian HEV genotype 3.

View Article: PubMed Central - PubMed

Affiliation: Shandong Agricultural University, Shandong, People's Republic of China.

ABSTRACT
Avian hepatitis E virus (HEV) has been identified in chickens; however, only 4 complete or near-complete genomic sequences have been reported. We found that the near-complete genomic sequence of avian HEV in chickens from China shared the highest identity (98.3%) with avian HEV from Europe and belonged to avian HEV genotype 3.

Show MeSH
Related in: MedlinePlus