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Family outbreak of Shiga toxin-producing Escherichia coli O123:H-, France, 2009.

King LA, Filliol-Toutain I, Mariani-Kurkidjian P, Vaillant V, Vernozy-Rozand C, Ganet S, Pihier N, Niaudet P, de Valk H - Emerging Infect. Dis. (2010)

View Article: PubMed Central - PubMed

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A food history indicated that the 2 patients had shared an undercooked ground beef burger 4–5 days before symptom onset... The patients’ parents also ate burgers from the same package (box); they did not report any gastrointestinal symptoms... A nonmotile strain of STEC stx2 eae ehxA, which was not serotypeable by the panel of 22 serum samples, was identified in fecal samples from patients and in the remaining ground beef... A clinical strain and a ground beef STEC strain were sent to the World Health Organization Collaborating Centre for Reference and Research on Escherichia and Klebsiella in Copenhagen, Denmark, in December 2009 for analysis... The clinical strain was confirmed as STEC O123:H–, and the meat strain was confirmed as a nonmotile STEC rough type by serum agglutination... Both strains had virulence genes stx2a, eae, and ehxA (F... Scheutz, pers. comm.)... We identified a family outbreak of STEC O123:H– stx2a, eae ehxA infections associated with ingestion of undercooked ground beef... We report foodborne transmission of STEC O123:H– that resulted in a cluster of clinical cases of infection... Eating ground beef is a well-established mode of STEC transmission, particularly for serotype O157:H7... STEC serotype O123:H– has been isolated from feces of healthy lambs and sheep in Spain and in southwestern Australia and is considered to be among the predominant ovine STEC serotypes in these countries.

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Representative XbaI pulsed-field gel electrophoresis patterns of Shiga toxin–producing Escherichia coli (STEC) O123:H– strains isolated from patient fecal samples and strains isolated from ground beef obtained from patients’ home, France, 2009. Lanes M, XbaI-digested genomic DNA from Salmonella enterica serovar Braenderup H9812 used as molecular mass markers; lane 1, Shiga toxin–producing STEC O123:H– isolated from patient with hemolytic uremic syndrome; lane 2, STEC O123:H– isolated from patient with diarrhea; lanes 3–6, STEC O123:H– isolated from ground beef from patients’ home; lanes 7 and 8, STEC O123 reference strains; lane 9, STEC 0111 isolate not related to the strains of the patients; lane 10, STEC 0157:H7 isolate not related to the strains of the patients.
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Figure 1: Representative XbaI pulsed-field gel electrophoresis patterns of Shiga toxin–producing Escherichia coli (STEC) O123:H– strains isolated from patient fecal samples and strains isolated from ground beef obtained from patients’ home, France, 2009. Lanes M, XbaI-digested genomic DNA from Salmonella enterica serovar Braenderup H9812 used as molecular mass markers; lane 1, Shiga toxin–producing STEC O123:H– isolated from patient with hemolytic uremic syndrome; lane 2, STEC O123:H– isolated from patient with diarrhea; lanes 3–6, STEC O123:H– isolated from ground beef from patients’ home; lanes 7 and 8, STEC O123 reference strains; lane 9, STEC 0111 isolate not related to the strains of the patients; lane 10, STEC 0157:H7 isolate not related to the strains of the patients.

Mentions: A nonmotile strain of STEC stx2 eae ehxA, which was not serotypeable by the panel of 22 serum samples, was identified in fecal samples from patients and in the remaining ground beef. Molecular serotyping of clinical isolates and an isolate from the beef identified a strain of STEC O123:H2. Analysis by pulsed-field gel electrophoresis indicated that the clinical and meat isolates were genetically related (Figure). The level of STEC contamination in the meat was 30–40 CFU/g. All stored meat production samples tested were negative for STEC.


Family outbreak of Shiga toxin-producing Escherichia coli O123:H-, France, 2009.

King LA, Filliol-Toutain I, Mariani-Kurkidjian P, Vaillant V, Vernozy-Rozand C, Ganet S, Pihier N, Niaudet P, de Valk H - Emerging Infect. Dis. (2010)

Representative XbaI pulsed-field gel electrophoresis patterns of Shiga toxin–producing Escherichia coli (STEC) O123:H– strains isolated from patient fecal samples and strains isolated from ground beef obtained from patients’ home, France, 2009. Lanes M, XbaI-digested genomic DNA from Salmonella enterica serovar Braenderup H9812 used as molecular mass markers; lane 1, Shiga toxin–producing STEC O123:H– isolated from patient with hemolytic uremic syndrome; lane 2, STEC O123:H– isolated from patient with diarrhea; lanes 3–6, STEC O123:H– isolated from ground beef from patients’ home; lanes 7 and 8, STEC O123 reference strains; lane 9, STEC 0111 isolate not related to the strains of the patients; lane 10, STEC 0157:H7 isolate not related to the strains of the patients.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3294988&req=5

Figure 1: Representative XbaI pulsed-field gel electrophoresis patterns of Shiga toxin–producing Escherichia coli (STEC) O123:H– strains isolated from patient fecal samples and strains isolated from ground beef obtained from patients’ home, France, 2009. Lanes M, XbaI-digested genomic DNA from Salmonella enterica serovar Braenderup H9812 used as molecular mass markers; lane 1, Shiga toxin–producing STEC O123:H– isolated from patient with hemolytic uremic syndrome; lane 2, STEC O123:H– isolated from patient with diarrhea; lanes 3–6, STEC O123:H– isolated from ground beef from patients’ home; lanes 7 and 8, STEC O123 reference strains; lane 9, STEC 0111 isolate not related to the strains of the patients; lane 10, STEC 0157:H7 isolate not related to the strains of the patients.
Mentions: A nonmotile strain of STEC stx2 eae ehxA, which was not serotypeable by the panel of 22 serum samples, was identified in fecal samples from patients and in the remaining ground beef. Molecular serotyping of clinical isolates and an isolate from the beef identified a strain of STEC O123:H2. Analysis by pulsed-field gel electrophoresis indicated that the clinical and meat isolates were genetically related (Figure). The level of STEC contamination in the meat was 30–40 CFU/g. All stored meat production samples tested were negative for STEC.

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

A food history indicated that the 2 patients had shared an undercooked ground beef burger 4–5 days before symptom onset... The patients’ parents also ate burgers from the same package (box); they did not report any gastrointestinal symptoms... A nonmotile strain of STEC stx2 eae ehxA, which was not serotypeable by the panel of 22 serum samples, was identified in fecal samples from patients and in the remaining ground beef... A clinical strain and a ground beef STEC strain were sent to the World Health Organization Collaborating Centre for Reference and Research on Escherichia and Klebsiella in Copenhagen, Denmark, in December 2009 for analysis... The clinical strain was confirmed as STEC O123:H–, and the meat strain was confirmed as a nonmotile STEC rough type by serum agglutination... Both strains had virulence genes stx2a, eae, and ehxA (F... Scheutz, pers. comm.)... We identified a family outbreak of STEC O123:H– stx2a, eae ehxA infections associated with ingestion of undercooked ground beef... We report foodborne transmission of STEC O123:H– that resulted in a cluster of clinical cases of infection... Eating ground beef is a well-established mode of STEC transmission, particularly for serotype O157:H7... STEC serotype O123:H– has been isolated from feces of healthy lambs and sheep in Spain and in southwestern Australia and is considered to be among the predominant ovine STEC serotypes in these countries.

Show MeSH
Related in: MedlinePlus