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Bovine GDF10 gene polymorphism analysis and its association with body measurement traits in Chinese indigenous cattle.

Adoligbe C, Zan L, Farougou S, Wang H, Ujjan JA - Mol. Biol. Rep. (2011)

Bottom Line: They showed 2 genotypes namely respectively (GG, GA) and (PP and PB).A11471G SNP is a missense mutation leading to the change of Alanine to Threonine amino acid.It showed three genotypes namely AA, BB and AB.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, People's Republic of China. adolcam83@yahoo.fr

ABSTRACT
The objective of this research was to detect bovine GDF10 gene polymorphism and analyze its association with body measurement traits (BMT) of animals sampled from 6 different Chinese indigenous cattle populations. The populations included Xuelong (Xl), Luxi (Lx), Qinchuan (Qc), Jiaxian red (Jx), Xianang (Xn) and Nanyang (Ny). Blood samples were taken from a total of 417 female animals stratified into age categories of 12-36 months. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was employed to find out GDF10 single polymorphism nucleotide (SNPs) and explore their possible association with BMT. Sequence analysis of GDF10 gene revealed 3 SNPs in total: 1 in exon1 (G142A) and 2 in exon3 (A11471G, and T12495C). G142A and T12495C SNPs are both synonymous mutation. They showed 2 genotypes namely respectively (GG, GA) and (PP and PB). A11471G SNP is a missense mutation leading to the change of Alanine to Threonine amino acid. It showed three genotypes namely AA, BB and AB. Analysis of association of polymorphism with body measurement traits at the three locus showed that there were significant effects on BMT in Qc, Jx and Ny cattle population. These results suggest that the GDF10 gene might have potential effects on body measurement traits in the above mentioned cattle populations and could be used for marker-assisted selection.

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a SSCP electrophoresis pattern of GDF10 gene exon3 (12495 bp locus). b The sequencing map of the novel SNP of bovine GDF10 exon3 (12495 bp locus)
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Fig3: a SSCP electrophoresis pattern of GDF10 gene exon3 (12495 bp locus). b The sequencing map of the novel SNP of bovine GDF10 exon3 (12495 bp locus)

Mentions: 10 pairs of PCR primers have been designed to amplify a DNA sequence from GDF10 gene exon1, 2 and 3. Sequence analysis of GDF10 gene revealed 3 SNPs in total, 1 in exon1 (G142A) and 2 in exon3 (A11471G and T12495C). A11471G SNP showed 3 genotypes namely AA, AB and BB (Fig. 2a), whereas both G142A SNP and T12495C SNP showed two genotypes namely GG and GA (Fig. 1a) and PB and PP (Fig. 3a) respectively.Fig. 1


Bovine GDF10 gene polymorphism analysis and its association with body measurement traits in Chinese indigenous cattle.

Adoligbe C, Zan L, Farougou S, Wang H, Ujjan JA - Mol. Biol. Rep. (2011)

a SSCP electrophoresis pattern of GDF10 gene exon3 (12495 bp locus). b The sequencing map of the novel SNP of bovine GDF10 exon3 (12495 bp locus)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3294207&req=5

Fig3: a SSCP electrophoresis pattern of GDF10 gene exon3 (12495 bp locus). b The sequencing map of the novel SNP of bovine GDF10 exon3 (12495 bp locus)
Mentions: 10 pairs of PCR primers have been designed to amplify a DNA sequence from GDF10 gene exon1, 2 and 3. Sequence analysis of GDF10 gene revealed 3 SNPs in total, 1 in exon1 (G142A) and 2 in exon3 (A11471G and T12495C). A11471G SNP showed 3 genotypes namely AA, AB and BB (Fig. 2a), whereas both G142A SNP and T12495C SNP showed two genotypes namely GG and GA (Fig. 1a) and PB and PP (Fig. 3a) respectively.Fig. 1

Bottom Line: They showed 2 genotypes namely respectively (GG, GA) and (PP and PB).A11471G SNP is a missense mutation leading to the change of Alanine to Threonine amino acid.It showed three genotypes namely AA, BB and AB.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, People's Republic of China. adolcam83@yahoo.fr

ABSTRACT
The objective of this research was to detect bovine GDF10 gene polymorphism and analyze its association with body measurement traits (BMT) of animals sampled from 6 different Chinese indigenous cattle populations. The populations included Xuelong (Xl), Luxi (Lx), Qinchuan (Qc), Jiaxian red (Jx), Xianang (Xn) and Nanyang (Ny). Blood samples were taken from a total of 417 female animals stratified into age categories of 12-36 months. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was employed to find out GDF10 single polymorphism nucleotide (SNPs) and explore their possible association with BMT. Sequence analysis of GDF10 gene revealed 3 SNPs in total: 1 in exon1 (G142A) and 2 in exon3 (A11471G, and T12495C). G142A and T12495C SNPs are both synonymous mutation. They showed 2 genotypes namely respectively (GG, GA) and (PP and PB). A11471G SNP is a missense mutation leading to the change of Alanine to Threonine amino acid. It showed three genotypes namely AA, BB and AB. Analysis of association of polymorphism with body measurement traits at the three locus showed that there were significant effects on BMT in Qc, Jx and Ny cattle population. These results suggest that the GDF10 gene might have potential effects on body measurement traits in the above mentioned cattle populations and could be used for marker-assisted selection.

Show MeSH