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A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast.

Cao S, Xu W, Zhang N, Wang Y, Luo Y, He X, Huang K - PLoS ONE (2012)

Bottom Line: Proteins related to redox, cellular structure, membrane, energy and DNA repair were significantly increased.The relationship between the proteins and metabolites was analyzed by orthogonal projections to latent structures (OPLS).We found that the changes of the metabolites and the protein changes had relevant consistency.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of food safety and molecular biology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, People's Republic of China.

ABSTRACT
Grapefruit seed extract (GSE), which has powerful anti-fungal activity, can induce apoptosis in S. cerevisiae. The yeast cells underwent apoptosis as determined by testing for apoptotic markers of DNA cleavage and typical chromatin condensation by Terminal Deoxynucleotidyl Transferase-mediated dUTP Nick End Labeling (TUNEL) and 4,6'-diaminidino-2-phenylindole (DAPI) staining and electron microscopy. The changes of ΔΨmt (mitochondrial transmembrane potential) and ROS (reactive oxygen species) indicated that the mitochondria took part in the apoptotic process. Changes in this process detected by metabonomics and proteomics revealed that the yeast cells tenaciously resisted adversity. Proteins related to redox, cellular structure, membrane, energy and DNA repair were significantly increased. In this study, the relative changes in the levels of proteins and metabolites showed the tenacious resistance of yeast cells. However, GSE induced apoptosis in the yeast cells by destruction of the mitochondrial 60 S ribosomal protein, L14-A, and prevented the conversion of pantothenic acid to coenzyme A (CoA). The relationship between the proteins and metabolites was analyzed by orthogonal projections to latent structures (OPLS). We found that the changes of the metabolites and the protein changes had relevant consistency.

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Related in: MedlinePlus

1H NMR spectra of a typical cell extract.
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pone-0032943-g006: 1H NMR spectra of a typical cell extract.

Mentions: Analysis of the PCA and PLS-DA (data not shown) highlighted the regions of the single-pulse 1H NMR spectra that were influential discriminators in the separation and classification of the GSE-treated and control samples (Figure 6). The GSE-treated cells exhibited lower concentrations of glutamate and γ-Aminobutyrate and higher concentrations of proline, creatine, glucitol and pantothenate (Table 2).


A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast.

Cao S, Xu W, Zhang N, Wang Y, Luo Y, He X, Huang K - PLoS ONE (2012)

1H NMR spectra of a typical cell extract.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3293924&req=5

pone-0032943-g006: 1H NMR spectra of a typical cell extract.
Mentions: Analysis of the PCA and PLS-DA (data not shown) highlighted the regions of the single-pulse 1H NMR spectra that were influential discriminators in the separation and classification of the GSE-treated and control samples (Figure 6). The GSE-treated cells exhibited lower concentrations of glutamate and γ-Aminobutyrate and higher concentrations of proline, creatine, glucitol and pantothenate (Table 2).

Bottom Line: Proteins related to redox, cellular structure, membrane, energy and DNA repair were significantly increased.The relationship between the proteins and metabolites was analyzed by orthogonal projections to latent structures (OPLS).We found that the changes of the metabolites and the protein changes had relevant consistency.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of food safety and molecular biology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, People's Republic of China.

ABSTRACT
Grapefruit seed extract (GSE), which has powerful anti-fungal activity, can induce apoptosis in S. cerevisiae. The yeast cells underwent apoptosis as determined by testing for apoptotic markers of DNA cleavage and typical chromatin condensation by Terminal Deoxynucleotidyl Transferase-mediated dUTP Nick End Labeling (TUNEL) and 4,6'-diaminidino-2-phenylindole (DAPI) staining and electron microscopy. The changes of ΔΨmt (mitochondrial transmembrane potential) and ROS (reactive oxygen species) indicated that the mitochondria took part in the apoptotic process. Changes in this process detected by metabonomics and proteomics revealed that the yeast cells tenaciously resisted adversity. Proteins related to redox, cellular structure, membrane, energy and DNA repair were significantly increased. In this study, the relative changes in the levels of proteins and metabolites showed the tenacious resistance of yeast cells. However, GSE induced apoptosis in the yeast cells by destruction of the mitochondrial 60 S ribosomal protein, L14-A, and prevented the conversion of pantothenic acid to coenzyme A (CoA). The relationship between the proteins and metabolites was analyzed by orthogonal projections to latent structures (OPLS). We found that the changes of the metabolites and the protein changes had relevant consistency.

Show MeSH
Related in: MedlinePlus