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A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast.

Cao S, Xu W, Zhang N, Wang Y, Luo Y, He X, Huang K - PLoS ONE (2012)

Bottom Line: Proteins related to redox, cellular structure, membrane, energy and DNA repair were significantly increased.The relationship between the proteins and metabolites was analyzed by orthogonal projections to latent structures (OPLS).We found that the changes of the metabolites and the protein changes had relevant consistency.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of food safety and molecular biology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, People's Republic of China.

ABSTRACT
Grapefruit seed extract (GSE), which has powerful anti-fungal activity, can induce apoptosis in S. cerevisiae. The yeast cells underwent apoptosis as determined by testing for apoptotic markers of DNA cleavage and typical chromatin condensation by Terminal Deoxynucleotidyl Transferase-mediated dUTP Nick End Labeling (TUNEL) and 4,6'-diaminidino-2-phenylindole (DAPI) staining and electron microscopy. The changes of ΔΨmt (mitochondrial transmembrane potential) and ROS (reactive oxygen species) indicated that the mitochondria took part in the apoptotic process. Changes in this process detected by metabonomics and proteomics revealed that the yeast cells tenaciously resisted adversity. Proteins related to redox, cellular structure, membrane, energy and DNA repair were significantly increased. In this study, the relative changes in the levels of proteins and metabolites showed the tenacious resistance of yeast cells. However, GSE induced apoptosis in the yeast cells by destruction of the mitochondrial 60 S ribosomal protein, L14-A, and prevented the conversion of pantothenic acid to coenzyme A (CoA). The relationship between the proteins and metabolites was analyzed by orthogonal projections to latent structures (OPLS). We found that the changes of the metabolites and the protein changes had relevant consistency.

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Related in: MedlinePlus

S. cerevisiae intracellular ROS.A–D, S. cerevisiae treated with 0.13 mg/mL of GSE for 0 h, 1 h, 2 h, and 3 h.
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pone-0032943-g005: S. cerevisiae intracellular ROS.A–D, S. cerevisiae treated with 0.13 mg/mL of GSE for 0 h, 1 h, 2 h, and 3 h.

Mentions: The cells were treated with or without 0.13 mg/mL GSE for 0–3 h, mixed with the ROS indicator DCFH-DA and subjected to FACS analysis (Figure 5). The cells that were not treated with GSE showed low intracellular ROS levels, but GSE-treated cells showed a significant increase in the amount of ROS after treatment with GSE for 2 h. The ROS levels declined slightly after 3 h.


A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast.

Cao S, Xu W, Zhang N, Wang Y, Luo Y, He X, Huang K - PLoS ONE (2012)

S. cerevisiae intracellular ROS.A–D, S. cerevisiae treated with 0.13 mg/mL of GSE for 0 h, 1 h, 2 h, and 3 h.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3293924&req=5

pone-0032943-g005: S. cerevisiae intracellular ROS.A–D, S. cerevisiae treated with 0.13 mg/mL of GSE for 0 h, 1 h, 2 h, and 3 h.
Mentions: The cells were treated with or without 0.13 mg/mL GSE for 0–3 h, mixed with the ROS indicator DCFH-DA and subjected to FACS analysis (Figure 5). The cells that were not treated with GSE showed low intracellular ROS levels, but GSE-treated cells showed a significant increase in the amount of ROS after treatment with GSE for 2 h. The ROS levels declined slightly after 3 h.

Bottom Line: Proteins related to redox, cellular structure, membrane, energy and DNA repair were significantly increased.The relationship between the proteins and metabolites was analyzed by orthogonal projections to latent structures (OPLS).We found that the changes of the metabolites and the protein changes had relevant consistency.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of food safety and molecular biology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, People's Republic of China.

ABSTRACT
Grapefruit seed extract (GSE), which has powerful anti-fungal activity, can induce apoptosis in S. cerevisiae. The yeast cells underwent apoptosis as determined by testing for apoptotic markers of DNA cleavage and typical chromatin condensation by Terminal Deoxynucleotidyl Transferase-mediated dUTP Nick End Labeling (TUNEL) and 4,6'-diaminidino-2-phenylindole (DAPI) staining and electron microscopy. The changes of ΔΨmt (mitochondrial transmembrane potential) and ROS (reactive oxygen species) indicated that the mitochondria took part in the apoptotic process. Changes in this process detected by metabonomics and proteomics revealed that the yeast cells tenaciously resisted adversity. Proteins related to redox, cellular structure, membrane, energy and DNA repair were significantly increased. In this study, the relative changes in the levels of proteins and metabolites showed the tenacious resistance of yeast cells. However, GSE induced apoptosis in the yeast cells by destruction of the mitochondrial 60 S ribosomal protein, L14-A, and prevented the conversion of pantothenic acid to coenzyme A (CoA). The relationship between the proteins and metabolites was analyzed by orthogonal projections to latent structures (OPLS). We found that the changes of the metabolites and the protein changes had relevant consistency.

Show MeSH
Related in: MedlinePlus