Limits...
Aloe vera induced biomimetic assemblage of nucleobase into nanosized particles.

Chauhan A, Zubair S, Sherwani A, Owais M - PLoS ONE (2012)

Bottom Line: Transmission electron microscopy and atomic force microscopic techniques confirmed nano-size of the synthesized particles.Importantly, the nano-assembled 5-FU retained its anticancer action against various cancerous cell lines.In the present study, we have explored the potential of biomimetic synthesis of nanoparticles employing organic molecules with the hope that such developments will be helpful to introduce novel nano-particle formulations that will not only be more effective but would also be devoid of nano-particle associated putative toxicity constraints.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.

ABSTRACT

Aim: Biomimetic nano-assembly formation offers a convenient and bio friendly approach to fabricate complex structures from simple components with sub-nanometer precision. Recently, biomimetic (employing microorganism/plants) synthesis of metal and inorganic materials nano-particles has emerged as a simple and viable strategy. In the present study, we have extended biological synthesis of nano-particles to organic molecules, namely the anticancer agent 5-fluorouracil (5-FU), using Aloe vera leaf extract.

Methodology: The 5-FU nano- particles synthesized by using Aloe vera leaf extract were characterized by UV, FT-IR and fluorescence spectroscopic techniques. The size and shape of the synthesized nanoparticles were determined by TEM, while crystalline nature of 5-FU particles was established by X-ray diffraction study. The cytotoxic effects of 5-FU nanoparticles were assessed against HT-29 and Caco-2 (human adenocarcinoma colorectal) cell lines.

Results: Transmission electron microscopy and atomic force microscopic techniques confirmed nano-size of the synthesized particles. Importantly, the nano-assembled 5-FU retained its anticancer action against various cancerous cell lines.

Conclusion: In the present study, we have explored the potential of biomimetic synthesis of nanoparticles employing organic molecules with the hope that such developments will be helpful to introduce novel nano-particle formulations that will not only be more effective but would also be devoid of nano-particle associated putative toxicity constraints.

Show MeSH

Related in: MedlinePlus

Release profile of 5-FU nano-particles under various conditions.To examine the release kinetics of 5-FU nano-particles, multiple samples of the formulation were dispensed into various micro vials. To each vial, 1.0 ml of release medium (20 mM sterile PBS, serum or histidine buffer) was dispensed. After stipulated time period, the suspension was centrifuged and an aliquot was analyzed for 5-FU content spectrophotometrically as described in Methodology section.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3293877&req=5

pone-0032049-g005: Release profile of 5-FU nano-particles under various conditions.To examine the release kinetics of 5-FU nano-particles, multiple samples of the formulation were dispensed into various micro vials. To each vial, 1.0 ml of release medium (20 mM sterile PBS, serum or histidine buffer) was dispensed. After stipulated time period, the suspension was centrifuged and an aliquot was analyzed for 5-FU content spectrophotometrically as described in Methodology section.

Mentions: To show that the nano-assemblies can act as a reservoir of 5-FU and facilitate the sustained release of the drug for extended time period, release profile of nano-particles was examined. For this multiple samples of 5-FU nano-particles were dispensed in various micro vials. The release profile showed slow and sustained release of drug over an extended time period. The release kinetic study indicates stability of nano-particles under different pH and temperature conditions. The nano-particles were found to withstand plasma components for a period of more than 120 hr resulting in release of less than 40% of the total drug (Figure 5).


Aloe vera induced biomimetic assemblage of nucleobase into nanosized particles.

Chauhan A, Zubair S, Sherwani A, Owais M - PLoS ONE (2012)

Release profile of 5-FU nano-particles under various conditions.To examine the release kinetics of 5-FU nano-particles, multiple samples of the formulation were dispensed into various micro vials. To each vial, 1.0 ml of release medium (20 mM sterile PBS, serum or histidine buffer) was dispensed. After stipulated time period, the suspension was centrifuged and an aliquot was analyzed for 5-FU content spectrophotometrically as described in Methodology section.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3293877&req=5

pone-0032049-g005: Release profile of 5-FU nano-particles under various conditions.To examine the release kinetics of 5-FU nano-particles, multiple samples of the formulation were dispensed into various micro vials. To each vial, 1.0 ml of release medium (20 mM sterile PBS, serum or histidine buffer) was dispensed. After stipulated time period, the suspension was centrifuged and an aliquot was analyzed for 5-FU content spectrophotometrically as described in Methodology section.
Mentions: To show that the nano-assemblies can act as a reservoir of 5-FU and facilitate the sustained release of the drug for extended time period, release profile of nano-particles was examined. For this multiple samples of 5-FU nano-particles were dispensed in various micro vials. The release profile showed slow and sustained release of drug over an extended time period. The release kinetic study indicates stability of nano-particles under different pH and temperature conditions. The nano-particles were found to withstand plasma components for a period of more than 120 hr resulting in release of less than 40% of the total drug (Figure 5).

Bottom Line: Transmission electron microscopy and atomic force microscopic techniques confirmed nano-size of the synthesized particles.Importantly, the nano-assembled 5-FU retained its anticancer action against various cancerous cell lines.In the present study, we have explored the potential of biomimetic synthesis of nanoparticles employing organic molecules with the hope that such developments will be helpful to introduce novel nano-particle formulations that will not only be more effective but would also be devoid of nano-particle associated putative toxicity constraints.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.

ABSTRACT

Aim: Biomimetic nano-assembly formation offers a convenient and bio friendly approach to fabricate complex structures from simple components with sub-nanometer precision. Recently, biomimetic (employing microorganism/plants) synthesis of metal and inorganic materials nano-particles has emerged as a simple and viable strategy. In the present study, we have extended biological synthesis of nano-particles to organic molecules, namely the anticancer agent 5-fluorouracil (5-FU), using Aloe vera leaf extract.

Methodology: The 5-FU nano- particles synthesized by using Aloe vera leaf extract were characterized by UV, FT-IR and fluorescence spectroscopic techniques. The size and shape of the synthesized nanoparticles were determined by TEM, while crystalline nature of 5-FU particles was established by X-ray diffraction study. The cytotoxic effects of 5-FU nanoparticles were assessed against HT-29 and Caco-2 (human adenocarcinoma colorectal) cell lines.

Results: Transmission electron microscopy and atomic force microscopic techniques confirmed nano-size of the synthesized particles. Importantly, the nano-assembled 5-FU retained its anticancer action against various cancerous cell lines.

Conclusion: In the present study, we have explored the potential of biomimetic synthesis of nanoparticles employing organic molecules with the hope that such developments will be helpful to introduce novel nano-particle formulations that will not only be more effective but would also be devoid of nano-particle associated putative toxicity constraints.

Show MeSH
Related in: MedlinePlus