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The highly virulent 2006 Norwegian EHEC O103:H25 outbreak strain is related to the 2011 German O104:H4 outbreak strain.

L'Abée-Lund TM, Jørgensen HJ, O'Sullivan K, Bohlin J, Ligård G, Granum PE, Lindbäck T - PLoS ONE (2012)

Bottom Line: All the other outbreak associated isolates, including all food isolates, were stx-negative, and carried a different phage replacing the Stx2 phage.The sequence of the early region of this phage was not retrieved from the bacterial host genome, and the origin of the phage is unknown.The contaminated food most likely contained a mixture of E. coli O103:H25 cells with either one of the phages.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, Oslo, Norway.

ABSTRACT
In 2006, a severe foodborne EHEC outbreak occured in Norway. Seventeen cases were recorded and the HUS frequency was 60%. The causative strain, Esherichia coli O103:H25, is considered to be particularly virulent. Sequencing of the outbreak strain revealed resemblance to the 2011 German outbreak strain E. coli O104:H4, both in genome and Shiga toxin 2-encoding (Stx2) phage sequence. The nucleotide identity between the Stx2 phages from the Norwegian and German outbreak strains was 90%. During the 2006 outbreak, stx(2)-positive O103:H25 E. coli was isolated from two patients. All the other outbreak associated isolates, including all food isolates, were stx-negative, and carried a different phage replacing the Stx2 phage. This phage was of similar size to the Stx2 phage, but had a distinctive early phage region and no stx gene. The sequence of the early region of this phage was not retrieved from the bacterial host genome, and the origin of the phage is unknown. The contaminated food most likely contained a mixture of E. coli O103:H25 cells with either one of the phages.

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Comparison of Stx2 phage and stx-negative phage.Comparison of Stx2 phage and stx-negative phage from EHEC O103:H25 NOS (A) and E. coli O103:H25 NVH-848 (B), respectively. The Stx2 phage is sequenced, while the illustration of the stx-negative phage is based on sequence (23 kb) and RFLP pattern (illustrated by arrow).
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pone-0031413-g003: Comparison of Stx2 phage and stx-negative phage.Comparison of Stx2 phage and stx-negative phage from EHEC O103:H25 NOS (A) and E. coli O103:H25 NVH-848 (B), respectively. The Stx2 phage is sequenced, while the illustration of the stx-negative phage is based on sequence (23 kb) and RFLP pattern (illustrated by arrow).

Mentions: To retrieve the genome sequence of the Stx2 phage, contigs from the EHEC O103:H25 NOS genome sequence containing Stx2 phage DNA were assembled, followed by gap closure PCRs and sequencing. The Stx2 phage genome is 60595 bp in length with a G+C content of 50%. A schematic view of the Stx2 phage is shown in Figure 3. The entire phage sequence was blasted against the sequence of the Stx2 phages of EAEC O104:H4 str C227-11, EHEC O103:H2 str 12009 and EHEC O157:H7 EDL933, and an ACT comparison is shown in Figure 4. The nucleotide identity to the Stx2 phages of EAEC O104:H4 str C227-11 [2] and EHEC O103:H2 str 12009 [7] is 90 and 88%, respectively, and covers the entire sequence, while the similarity to the reference EHEC O157:H7 EDL933 phage is limited to the 35 kb late region (Figure 4).


The highly virulent 2006 Norwegian EHEC O103:H25 outbreak strain is related to the 2011 German O104:H4 outbreak strain.

L'Abée-Lund TM, Jørgensen HJ, O'Sullivan K, Bohlin J, Ligård G, Granum PE, Lindbäck T - PLoS ONE (2012)

Comparison of Stx2 phage and stx-negative phage.Comparison of Stx2 phage and stx-negative phage from EHEC O103:H25 NOS (A) and E. coli O103:H25 NVH-848 (B), respectively. The Stx2 phage is sequenced, while the illustration of the stx-negative phage is based on sequence (23 kb) and RFLP pattern (illustrated by arrow).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3293862&req=5

pone-0031413-g003: Comparison of Stx2 phage and stx-negative phage.Comparison of Stx2 phage and stx-negative phage from EHEC O103:H25 NOS (A) and E. coli O103:H25 NVH-848 (B), respectively. The Stx2 phage is sequenced, while the illustration of the stx-negative phage is based on sequence (23 kb) and RFLP pattern (illustrated by arrow).
Mentions: To retrieve the genome sequence of the Stx2 phage, contigs from the EHEC O103:H25 NOS genome sequence containing Stx2 phage DNA were assembled, followed by gap closure PCRs and sequencing. The Stx2 phage genome is 60595 bp in length with a G+C content of 50%. A schematic view of the Stx2 phage is shown in Figure 3. The entire phage sequence was blasted against the sequence of the Stx2 phages of EAEC O104:H4 str C227-11, EHEC O103:H2 str 12009 and EHEC O157:H7 EDL933, and an ACT comparison is shown in Figure 4. The nucleotide identity to the Stx2 phages of EAEC O104:H4 str C227-11 [2] and EHEC O103:H2 str 12009 [7] is 90 and 88%, respectively, and covers the entire sequence, while the similarity to the reference EHEC O157:H7 EDL933 phage is limited to the 35 kb late region (Figure 4).

Bottom Line: All the other outbreak associated isolates, including all food isolates, were stx-negative, and carried a different phage replacing the Stx2 phage.The sequence of the early region of this phage was not retrieved from the bacterial host genome, and the origin of the phage is unknown.The contaminated food most likely contained a mixture of E. coli O103:H25 cells with either one of the phages.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, Oslo, Norway.

ABSTRACT
In 2006, a severe foodborne EHEC outbreak occured in Norway. Seventeen cases were recorded and the HUS frequency was 60%. The causative strain, Esherichia coli O103:H25, is considered to be particularly virulent. Sequencing of the outbreak strain revealed resemblance to the 2011 German outbreak strain E. coli O104:H4, both in genome and Shiga toxin 2-encoding (Stx2) phage sequence. The nucleotide identity between the Stx2 phages from the Norwegian and German outbreak strains was 90%. During the 2006 outbreak, stx(2)-positive O103:H25 E. coli was isolated from two patients. All the other outbreak associated isolates, including all food isolates, were stx-negative, and carried a different phage replacing the Stx2 phage. This phage was of similar size to the Stx2 phage, but had a distinctive early phage region and no stx gene. The sequence of the early region of this phage was not retrieved from the bacterial host genome, and the origin of the phage is unknown. The contaminated food most likely contained a mixture of E. coli O103:H25 cells with either one of the phages.

Show MeSH
Related in: MedlinePlus