Limits...
ArgR of Streptomyces coelicolor is a versatile regulator.

Pérez-Redondo R, Rodríguez-García A, Botas A, Santamarta I, Martín JF, Liras P - PLoS ONE (2012)

Bottom Line: Arginine and pyrimidine biosynthesis genes were derepressed by the lack of ArgR, while no strong effect on expression resulted on arginine supplementation.The transcriptomic data were validated by either reverse transcription-PCR, expression of the gene-promoter coupled to the luciferase gene, proteomic or by electrophoresis mobility shift assay (EMSA) using pure Strep-tagged ArgR.Other genes, including genes encoding regulatory proteins, possess a DNA sequence formed by a single ARG-box which responds to ArgR, as validated by EMSA.

View Article: PubMed Central - PubMed

Affiliation: Área de Microbiología, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, León, Spain.

ABSTRACT
ArgR is the regulator of arginine biosynthesis genes in Streptomyces species. Transcriptomic comparison by microarrays has been made between Streptomyces coelicolor M145 and its mutant S. coelicolor ΔargR under control, unsupplemented conditions, and in the presence of arginine. Expression of 459 genes was different in transcriptomic assays, but only 27 genes were affected by arginine supplementation. Arginine and pyrimidine biosynthesis genes were derepressed by the lack of ArgR, while no strong effect on expression resulted on arginine supplementation. Several nitrogen metabolism genes expression as glnK, glnA and glnII, were downregulated in S. coelicolor ΔargR. In addition, downregulation of genes for the yellow type I polyketide CPK antibiotic and for the antibiotic regulatory genes afsS and scbR was observed. The transcriptomic data were validated by either reverse transcription-PCR, expression of the gene-promoter coupled to the luciferase gene, proteomic or by electrophoresis mobility shift assay (EMSA) using pure Strep-tagged ArgR. Two ARG-boxes in the arginine operon genes suggest that these genes are more tightly controlled. Other genes, including genes encoding regulatory proteins, possess a DNA sequence formed by a single ARG-box which responds to ArgR, as validated by EMSA.

Show MeSH
Differential expression of genes related to morphology and secondary metabolism.(A) Transcriptomic of genes for rodlins and chaplins. The cluster chp–rdl is shown above with differentially expressed genes in black. (B) Transcriptomic of the genes for CPK biosynthesis. The cpk cluster is shown above. Genes with differential expression are indicated in black. Included are the genes for scbR, for a butyrolactone receptor protein, and scF, for a putative secreted FAD-binding protein. A, B, C and D correspond to the conditions indicated in Fig. 3.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3293853&req=5

pone-0032697-g006: Differential expression of genes related to morphology and secondary metabolism.(A) Transcriptomic of genes for rodlins and chaplins. The cluster chp–rdl is shown above with differentially expressed genes in black. (B) Transcriptomic of the genes for CPK biosynthesis. The cpk cluster is shown above. Genes with differential expression are indicated in black. Included are the genes for scbR, for a butyrolactone receptor protein, and scF, for a putative secreted FAD-binding protein. A, B, C and D correspond to the conditions indicated in Fig. 3.

Mentions: A very strong effect due to the lack of ArgR was observed on the biosynthesis of rodlins and chaplins, required to form aerial mycelium and spores [28], and on the genes encoding the yellow polyketide pigment CPK [29] (Fig. 6). Genes for chaplins are located in five different sites in the genome being chpA and chpD clustered with rdlA and rdlB for rodlins (SCO2716–2719). Although expression of chpF and chpA was not affected, the other genes showed profiles of the I.4 type (chpG and chpE, SCO2699 and SCO1800, respectively) and I.5 type (chpH, rdlA and chpC, SCO1675, SCO2718 and SCO1674, respectively).


ArgR of Streptomyces coelicolor is a versatile regulator.

Pérez-Redondo R, Rodríguez-García A, Botas A, Santamarta I, Martín JF, Liras P - PLoS ONE (2012)

Differential expression of genes related to morphology and secondary metabolism.(A) Transcriptomic of genes for rodlins and chaplins. The cluster chp–rdl is shown above with differentially expressed genes in black. (B) Transcriptomic of the genes for CPK biosynthesis. The cpk cluster is shown above. Genes with differential expression are indicated in black. Included are the genes for scbR, for a butyrolactone receptor protein, and scF, for a putative secreted FAD-binding protein. A, B, C and D correspond to the conditions indicated in Fig. 3.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3293853&req=5

pone-0032697-g006: Differential expression of genes related to morphology and secondary metabolism.(A) Transcriptomic of genes for rodlins and chaplins. The cluster chp–rdl is shown above with differentially expressed genes in black. (B) Transcriptomic of the genes for CPK biosynthesis. The cpk cluster is shown above. Genes with differential expression are indicated in black. Included are the genes for scbR, for a butyrolactone receptor protein, and scF, for a putative secreted FAD-binding protein. A, B, C and D correspond to the conditions indicated in Fig. 3.
Mentions: A very strong effect due to the lack of ArgR was observed on the biosynthesis of rodlins and chaplins, required to form aerial mycelium and spores [28], and on the genes encoding the yellow polyketide pigment CPK [29] (Fig. 6). Genes for chaplins are located in five different sites in the genome being chpA and chpD clustered with rdlA and rdlB for rodlins (SCO2716–2719). Although expression of chpF and chpA was not affected, the other genes showed profiles of the I.4 type (chpG and chpE, SCO2699 and SCO1800, respectively) and I.5 type (chpH, rdlA and chpC, SCO1675, SCO2718 and SCO1674, respectively).

Bottom Line: Arginine and pyrimidine biosynthesis genes were derepressed by the lack of ArgR, while no strong effect on expression resulted on arginine supplementation.The transcriptomic data were validated by either reverse transcription-PCR, expression of the gene-promoter coupled to the luciferase gene, proteomic or by electrophoresis mobility shift assay (EMSA) using pure Strep-tagged ArgR.Other genes, including genes encoding regulatory proteins, possess a DNA sequence formed by a single ARG-box which responds to ArgR, as validated by EMSA.

View Article: PubMed Central - PubMed

Affiliation: Área de Microbiología, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, León, Spain.

ABSTRACT
ArgR is the regulator of arginine biosynthesis genes in Streptomyces species. Transcriptomic comparison by microarrays has been made between Streptomyces coelicolor M145 and its mutant S. coelicolor ΔargR under control, unsupplemented conditions, and in the presence of arginine. Expression of 459 genes was different in transcriptomic assays, but only 27 genes were affected by arginine supplementation. Arginine and pyrimidine biosynthesis genes were derepressed by the lack of ArgR, while no strong effect on expression resulted on arginine supplementation. Several nitrogen metabolism genes expression as glnK, glnA and glnII, were downregulated in S. coelicolor ΔargR. In addition, downregulation of genes for the yellow type I polyketide CPK antibiotic and for the antibiotic regulatory genes afsS and scbR was observed. The transcriptomic data were validated by either reverse transcription-PCR, expression of the gene-promoter coupled to the luciferase gene, proteomic or by electrophoresis mobility shift assay (EMSA) using pure Strep-tagged ArgR. Two ARG-boxes in the arginine operon genes suggest that these genes are more tightly controlled. Other genes, including genes encoding regulatory proteins, possess a DNA sequence formed by a single ARG-box which responds to ArgR, as validated by EMSA.

Show MeSH