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ArgR of Streptomyces coelicolor is a versatile regulator.

Pérez-Redondo R, Rodríguez-García A, Botas A, Santamarta I, Martín JF, Liras P - PLoS ONE (2012)

Bottom Line: Arginine and pyrimidine biosynthesis genes were derepressed by the lack of ArgR, while no strong effect on expression resulted on arginine supplementation.The transcriptomic data were validated by either reverse transcription-PCR, expression of the gene-promoter coupled to the luciferase gene, proteomic or by electrophoresis mobility shift assay (EMSA) using pure Strep-tagged ArgR.Other genes, including genes encoding regulatory proteins, possess a DNA sequence formed by a single ARG-box which responds to ArgR, as validated by EMSA.

View Article: PubMed Central - PubMed

Affiliation: Área de Microbiología, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, León, Spain.

ABSTRACT
ArgR is the regulator of arginine biosynthesis genes in Streptomyces species. Transcriptomic comparison by microarrays has been made between Streptomyces coelicolor M145 and its mutant S. coelicolor ΔargR under control, unsupplemented conditions, and in the presence of arginine. Expression of 459 genes was different in transcriptomic assays, but only 27 genes were affected by arginine supplementation. Arginine and pyrimidine biosynthesis genes were derepressed by the lack of ArgR, while no strong effect on expression resulted on arginine supplementation. Several nitrogen metabolism genes expression as glnK, glnA and glnII, were downregulated in S. coelicolor ΔargR. In addition, downregulation of genes for the yellow type I polyketide CPK antibiotic and for the antibiotic regulatory genes afsS and scbR was observed. The transcriptomic data were validated by either reverse transcription-PCR, expression of the gene-promoter coupled to the luciferase gene, proteomic or by electrophoresis mobility shift assay (EMSA) using pure Strep-tagged ArgR. Two ARG-boxes in the arginine operon genes suggest that these genes are more tightly controlled. Other genes, including genes encoding regulatory proteins, possess a DNA sequence formed by a single ARG-box which responds to ArgR, as validated by EMSA.

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Transcription profiles.Genes differentially expressed in MG cultures of S. coelicolor M145 (A); S. coelicolor M145 supplemented with arginine (B); S. coelicolor ΔargR (C) and S. coelicolor ΔargR supplemented with arginine (D). The genes have been grouped in types and subtypes according with their expression profile. Each grey line corresponds to the change in transcription of a given gene. The thicker line is the transcription mean profile for all the genes in the proposed group.
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pone-0032697-g003: Transcription profiles.Genes differentially expressed in MG cultures of S. coelicolor M145 (A); S. coelicolor M145 supplemented with arginine (B); S. coelicolor ΔargR (C) and S. coelicolor ΔargR supplemented with arginine (D). The genes have been grouped in types and subtypes according with their expression profile. Each grey line corresponds to the change in transcription of a given gene. The thicker line is the transcription mean profile for all the genes in the proposed group.

Mentions: The transcriptional profiles analysis permitted to establish a classification of the genes into five types. The patterns of genes differentially transcribed in the two strains in unsupplemented or supplemented conditions fell mainly into types I and II (Fig. 3) with different modulations of the expression (subtypes 1–5). Forty-three genes showed the pattern of expression indicated in types III to V (Fig. 3). Here we will analyze general genes included in the five different types, while genes with specific functions will be analyzed in subsequent sections.


ArgR of Streptomyces coelicolor is a versatile regulator.

Pérez-Redondo R, Rodríguez-García A, Botas A, Santamarta I, Martín JF, Liras P - PLoS ONE (2012)

Transcription profiles.Genes differentially expressed in MG cultures of S. coelicolor M145 (A); S. coelicolor M145 supplemented with arginine (B); S. coelicolor ΔargR (C) and S. coelicolor ΔargR supplemented with arginine (D). The genes have been grouped in types and subtypes according with their expression profile. Each grey line corresponds to the change in transcription of a given gene. The thicker line is the transcription mean profile for all the genes in the proposed group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3293853&req=5

pone-0032697-g003: Transcription profiles.Genes differentially expressed in MG cultures of S. coelicolor M145 (A); S. coelicolor M145 supplemented with arginine (B); S. coelicolor ΔargR (C) and S. coelicolor ΔargR supplemented with arginine (D). The genes have been grouped in types and subtypes according with their expression profile. Each grey line corresponds to the change in transcription of a given gene. The thicker line is the transcription mean profile for all the genes in the proposed group.
Mentions: The transcriptional profiles analysis permitted to establish a classification of the genes into five types. The patterns of genes differentially transcribed in the two strains in unsupplemented or supplemented conditions fell mainly into types I and II (Fig. 3) with different modulations of the expression (subtypes 1–5). Forty-three genes showed the pattern of expression indicated in types III to V (Fig. 3). Here we will analyze general genes included in the five different types, while genes with specific functions will be analyzed in subsequent sections.

Bottom Line: Arginine and pyrimidine biosynthesis genes were derepressed by the lack of ArgR, while no strong effect on expression resulted on arginine supplementation.The transcriptomic data were validated by either reverse transcription-PCR, expression of the gene-promoter coupled to the luciferase gene, proteomic or by electrophoresis mobility shift assay (EMSA) using pure Strep-tagged ArgR.Other genes, including genes encoding regulatory proteins, possess a DNA sequence formed by a single ARG-box which responds to ArgR, as validated by EMSA.

View Article: PubMed Central - PubMed

Affiliation: Área de Microbiología, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, León, Spain.

ABSTRACT
ArgR is the regulator of arginine biosynthesis genes in Streptomyces species. Transcriptomic comparison by microarrays has been made between Streptomyces coelicolor M145 and its mutant S. coelicolor ΔargR under control, unsupplemented conditions, and in the presence of arginine. Expression of 459 genes was different in transcriptomic assays, but only 27 genes were affected by arginine supplementation. Arginine and pyrimidine biosynthesis genes were derepressed by the lack of ArgR, while no strong effect on expression resulted on arginine supplementation. Several nitrogen metabolism genes expression as glnK, glnA and glnII, were downregulated in S. coelicolor ΔargR. In addition, downregulation of genes for the yellow type I polyketide CPK antibiotic and for the antibiotic regulatory genes afsS and scbR was observed. The transcriptomic data were validated by either reverse transcription-PCR, expression of the gene-promoter coupled to the luciferase gene, proteomic or by electrophoresis mobility shift assay (EMSA) using pure Strep-tagged ArgR. Two ARG-boxes in the arginine operon genes suggest that these genes are more tightly controlled. Other genes, including genes encoding regulatory proteins, possess a DNA sequence formed by a single ARG-box which responds to ArgR, as validated by EMSA.

Show MeSH