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Suppressive effects of liquid crystal compounds on the growth of U937 human leukemic monocyte lymphoma cells.

Ishikawa J, Takahashi Y, Hazawa M, Fukushi Y, Yoshizawa A, Kashiwakura I - Cancer Cell Int. (2012)

Bottom Line: Some LCs showed cytostatic effects, suppressing cell growth via S-phase arrest and without apoptosis in U937 cells.The rate of LC-induced S-phase arrest was congruent with the decreased expression of MCM2, cyclin A, cyclin B, CDK2, phospho-CDK1 and Cdc25C.Our results revealed that some LCs showed cytotoxic properties against non-solid type tumor human leukemic cells via LC-induced S-phase arrest and decreasing expression of several cell cycle related proteins.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, 036-8203, Aomori Hirosaki, Japan. ikashi@cc.hirosaki-u.ac.jp.

ABSTRACT

Background: The aim of this study was to evaluate the biological and pharmaceutical activities of 14 amphiphilic liquid-crystalline compounds (LCs), i.e, phenylpyrimidine derivatives possessing D-glucamine and cyanobiphenyl derivatives with a terminal hydroxyl unit.

Results: The cytotoxic properties of the LCs on the cell growth, cell cycle distribution, and cell signaling pathway of U937 human leukemic monocyte lymphoma cells were assessed by flow cytometry and western blot analysis. Some LCs showed cytostatic effects, suppressing cell growth via S-phase arrest and without apoptosis in U937 cells. To investigate the mechanisms of the LC-induced S-phase arrest, proteins relevant to cell cycle regulation were investigated by western blot analysis. The rate of LC-induced S-phase arrest was congruent with the decreased expression of MCM2, cyclin A, cyclin B, CDK2, phospho-CDK1 and Cdc25C. Observed changes in cell cycle distribution by LC treated might be caused by insufficient preparation for G2/M transition. Considering the structure of the LCs, the rod-like molecules displaying cytotoxicity against U937 cells possessed flexible spacers with no bulky polar group attached via the flexible spacer.

Conclusions: Our results revealed that some LCs showed cytotoxic properties against non-solid type tumor human leukemic cells via LC-induced S-phase arrest and decreasing expression of several cell cycle related proteins.

No MeSH data available.


Related in: MedlinePlus

Effect of liquid crystalline compounds on the cell cycle distribution in U937 human leukemic monocyte lymphoma cells. [A] Flow cytometry analysis of U937 cells treated with each compounds for indicated times. Representative cytograms are shown. [B] The rate of S-phase in U937 cells was shown. Values are the mean ± standard deviation (S.D.) value of at least 3 separate experiments. *p < 0.05 by Student's t-test.
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Figure 4: Effect of liquid crystalline compounds on the cell cycle distribution in U937 human leukemic monocyte lymphoma cells. [A] Flow cytometry analysis of U937 cells treated with each compounds for indicated times. Representative cytograms are shown. [B] The rate of S-phase in U937 cells was shown. Values are the mean ± standard deviation (S.D.) value of at least 3 separate experiments. *p < 0.05 by Student's t-test.

Mentions: Since anti-tumor drugs usually affect the cell cycle distribution [11], the cell cycle distribution in U937 cells treated with LCs was analyzed by flow cytometry. As shown in Figure 4A, no modification of the cell cycle distribution was observed in LCs-treated cells compared to the controls at 3 h. However, LCs significantly induced the S-phase population at 6 and 12 h (Figure 4B). These data indicated that LCs induced S-phase arrest in U937 cells.


Suppressive effects of liquid crystal compounds on the growth of U937 human leukemic monocyte lymphoma cells.

Ishikawa J, Takahashi Y, Hazawa M, Fukushi Y, Yoshizawa A, Kashiwakura I - Cancer Cell Int. (2012)

Effect of liquid crystalline compounds on the cell cycle distribution in U937 human leukemic monocyte lymphoma cells. [A] Flow cytometry analysis of U937 cells treated with each compounds for indicated times. Representative cytograms are shown. [B] The rate of S-phase in U937 cells was shown. Values are the mean ± standard deviation (S.D.) value of at least 3 separate experiments. *p < 0.05 by Student's t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3293784&req=5

Figure 4: Effect of liquid crystalline compounds on the cell cycle distribution in U937 human leukemic monocyte lymphoma cells. [A] Flow cytometry analysis of U937 cells treated with each compounds for indicated times. Representative cytograms are shown. [B] The rate of S-phase in U937 cells was shown. Values are the mean ± standard deviation (S.D.) value of at least 3 separate experiments. *p < 0.05 by Student's t-test.
Mentions: Since anti-tumor drugs usually affect the cell cycle distribution [11], the cell cycle distribution in U937 cells treated with LCs was analyzed by flow cytometry. As shown in Figure 4A, no modification of the cell cycle distribution was observed in LCs-treated cells compared to the controls at 3 h. However, LCs significantly induced the S-phase population at 6 and 12 h (Figure 4B). These data indicated that LCs induced S-phase arrest in U937 cells.

Bottom Line: Some LCs showed cytostatic effects, suppressing cell growth via S-phase arrest and without apoptosis in U937 cells.The rate of LC-induced S-phase arrest was congruent with the decreased expression of MCM2, cyclin A, cyclin B, CDK2, phospho-CDK1 and Cdc25C.Our results revealed that some LCs showed cytotoxic properties against non-solid type tumor human leukemic cells via LC-induced S-phase arrest and decreasing expression of several cell cycle related proteins.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, 036-8203, Aomori Hirosaki, Japan. ikashi@cc.hirosaki-u.ac.jp.

ABSTRACT

Background: The aim of this study was to evaluate the biological and pharmaceutical activities of 14 amphiphilic liquid-crystalline compounds (LCs), i.e, phenylpyrimidine derivatives possessing D-glucamine and cyanobiphenyl derivatives with a terminal hydroxyl unit.

Results: The cytotoxic properties of the LCs on the cell growth, cell cycle distribution, and cell signaling pathway of U937 human leukemic monocyte lymphoma cells were assessed by flow cytometry and western blot analysis. Some LCs showed cytostatic effects, suppressing cell growth via S-phase arrest and without apoptosis in U937 cells. To investigate the mechanisms of the LC-induced S-phase arrest, proteins relevant to cell cycle regulation were investigated by western blot analysis. The rate of LC-induced S-phase arrest was congruent with the decreased expression of MCM2, cyclin A, cyclin B, CDK2, phospho-CDK1 and Cdc25C. Observed changes in cell cycle distribution by LC treated might be caused by insufficient preparation for G2/M transition. Considering the structure of the LCs, the rod-like molecules displaying cytotoxicity against U937 cells possessed flexible spacers with no bulky polar group attached via the flexible spacer.

Conclusions: Our results revealed that some LCs showed cytotoxic properties against non-solid type tumor human leukemic cells via LC-induced S-phase arrest and decreasing expression of several cell cycle related proteins.

No MeSH data available.


Related in: MedlinePlus