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Human Plasmodium knowlesi infection in Ranong province, southwestern border of Thailand.

Sermwittayawong N, Singh B, Nishibuchi M, Sawangjaroen N, Vuddhakul V - Malar. J. (2012)

Bottom Line: Nucleotide sequences of the csp gene derived from these two patients were identical and phylogenetically indistinguishable from other P. knowlesi sequences derived from monkeys and humans.Both patients worked in Koh Song, located in the Kawthoung district of Myanmar, which borders Thailand.This study indicates that transmission of P. knowlesi is occurring in the Ranong province of Thailand or the Kawthoung district of Myanmar.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand. natthawan.k@psu.ac.th

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Nested PCR screening of blood samples collected for Plasmodium knowlesi Lanes M = Molecular weight marker (100 bp ladder -New England Biolabs); lane 1 = negaitve control, lane 2 = positive control; lane 3-6 and 8 = negative blood samples; lane 7 and 9 = positive blood samples (M2/20 and M2/51 respectively).
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Figure 2: Nested PCR screening of blood samples collected for Plasmodium knowlesi Lanes M = Molecular weight marker (100 bp ladder -New England Biolabs); lane 1 = negaitve control, lane 2 = positive control; lane 3-6 and 8 = negative blood samples; lane 7 and 9 = positive blood samples (M2/20 and M2/51 respectively).

Mentions: A total of 419 blood samples were investigated and a 153-bp SSU rRNA fragment specific for P. knowlesi was detected in only two samples (designated as M2/20 and M2/51) (Figure 2). One sample had been obtained from a 45 year-old Thai male and the other was from a 30 year-old Myanmese male. Both samples were collected in the same period of time (June 2008) and were diagnosed by microscopy as P. vivax infections. These samples were examined for co-infection with other Plasmodium spp. by nested PCR using species-specific primers for P. falciparum, P. malariae, P. ovale and P. vivax [12]. Both samples were negative, confirming that they were each a single infection of P. knowlesi.


Human Plasmodium knowlesi infection in Ranong province, southwestern border of Thailand.

Sermwittayawong N, Singh B, Nishibuchi M, Sawangjaroen N, Vuddhakul V - Malar. J. (2012)

Nested PCR screening of blood samples collected for Plasmodium knowlesi Lanes M = Molecular weight marker (100 bp ladder -New England Biolabs); lane 1 = negaitve control, lane 2 = positive control; lane 3-6 and 8 = negative blood samples; lane 7 and 9 = positive blood samples (M2/20 and M2/51 respectively).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3293766&req=5

Figure 2: Nested PCR screening of blood samples collected for Plasmodium knowlesi Lanes M = Molecular weight marker (100 bp ladder -New England Biolabs); lane 1 = negaitve control, lane 2 = positive control; lane 3-6 and 8 = negative blood samples; lane 7 and 9 = positive blood samples (M2/20 and M2/51 respectively).
Mentions: A total of 419 blood samples were investigated and a 153-bp SSU rRNA fragment specific for P. knowlesi was detected in only two samples (designated as M2/20 and M2/51) (Figure 2). One sample had been obtained from a 45 year-old Thai male and the other was from a 30 year-old Myanmese male. Both samples were collected in the same period of time (June 2008) and were diagnosed by microscopy as P. vivax infections. These samples were examined for co-infection with other Plasmodium spp. by nested PCR using species-specific primers for P. falciparum, P. malariae, P. ovale and P. vivax [12]. Both samples were negative, confirming that they were each a single infection of P. knowlesi.

Bottom Line: Nucleotide sequences of the csp gene derived from these two patients were identical and phylogenetically indistinguishable from other P. knowlesi sequences derived from monkeys and humans.Both patients worked in Koh Song, located in the Kawthoung district of Myanmar, which borders Thailand.This study indicates that transmission of P. knowlesi is occurring in the Ranong province of Thailand or the Kawthoung district of Myanmar.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand. natthawan.k@psu.ac.th

Show MeSH
Related in: MedlinePlus